The present study was aimed to investigate sea lice infestation including morphometric, molecular identification, disease prevalence and pathological tissue alterations in wild Sea bass (Morone Labrax) naturally collected from Ezbet Elborg, Damietta province, Egypt from October 2018 to end of August 2019. Design: Descriptive study Fish: 120 wild seabass (Morone Labrax) Procedures: The collected fish from Ezbet Elborg area, Damietta province, Egypt were clinically and parasitological investigated for the prevalence of sea lice parasite with molecular identification of the collected parasite samples with study of the pathological alteration of the affected tissue.
Results:The study revealed that 48 fish (40%) of the examined fish were infested with Caligus clemensi, while the higher prevalence recorded during summer season (57%) followed spring (33%), autumn (10%) and no recording during winter. The molecular identification using 18-S rDNA was done with phylogenetic analysis compared with the previously recorded caligus species on database confirming the same results of parasitology. Histopathological examination of gills, buccal cavity revealed that the gill arch contained congested blood vessels beside edema, hemorrhage and inflammatory cells infiltration due to parasitic infestation.
Conclusions and clinical relevance:Caligus infestation in wild sea bass caused by Caligus clemensi lead to mortalities due to pathological tissue alterations in gills and buccal cavity.
For the time being, a growing number of exotic reptiles are kept as pets. Pet reptiles are often infected with parasites, some of which are potentially dangerous to humans. A total of 115 samples from reptile species (Wedge-Snouted skink, African Chameleon, Egg eating snake and Egyptian tortoise) were collected for examination. The results indicated that (48.7%) out of 115 infected with internal parasites .A wide range of internal parasites were detected including nematodes, cestodes and protozoa. Wedge-snouted skink showed the highest infection rate where it reached 82.3%, followed by African Chameleon (51.5%), Egg eating snake (23%) and Egyptian tortoise (22.7%). Various parasites were detected including Strongylid eggs, Spirurid eggs, Heterakid eggs, Oxyurid eggs, eggs of mites, Nyctotherus cysts, Cryptosporidium oocysts, Thelandros spp., Parapharyngodon spp., Raillietascarisvarani, Physaloptera tupinambae, Meteterakis saotomensis and Oochoristica spp.These results revealed that a routine parasitological examination should be done in such animals.
Background: This study was performed to determine the prevalence and to identify precisely Toxocara spp., which infects feral cats in Alexandria, Egypt based on morphological and molecular approaches.
Methods: This cross-sectional study was carried out on 100 feral cats trapped from different areas of Alexandria during 2018. Adult male and female worms were recovered from small intestinal contents after euthanasia and dissection of cats. Distinct morphological features were initially determined using available keys, and then after amplification and sequencing of the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene was carried out and phylogenetic trees were constructed.
Results: Forty out of 100 cats were infected with Toxocara spp. Intensity of infection ranged from 1 to 9 worms/cat, with a mean of 2.27±1.6. All isolates were confirmed as T. cati based on morphological features and the sequence of nad1 gene. Results of the current study clearly show that Egyptian T. cati isolate examined herein is genetically similar to those recorded in other countries.
Conclusion: The current work revealed high prevalence of T. cati in feral cats in the study area. This is the first genetic study that confirms T. cati from feral cats in Egypt. In addition, it demonstrated the suitability and need of genetic markers such as nad1 for identification of Toxocara spp. Furthermore highlights the public health importance of T. cati in Egypt.
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