This study aimed to sequence and annotate the complete mitochondrial DNA genome sequence of
Opsarius pulchellus.
Complete mitochondrial genome is a circular molecule of 16,552 bp in length, including 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a displacement loop region. Proportion of nucleotides in mitochondrial genome is 26.8% T, 26.9% C, 27.6% A, and 18.8% G, with an AT bias of 54.4%. Maximum-likelihood phylogenetic tree was reconstructed using the connected protein sequences of 13 PCGs of
O. pulchellus
and other 18 fishes. Result of phylogenetic analysis supports a close relationship between
O. pulchellus
and
Barilius bendelisis
(Hamilton, 1807). Fundamental genetic data of
O. pulchellus
would be beneficial for further studies on genetic diversity and phylogeny.
Danio roseus
, collected from Gadu River in Yingjiang area, Yunnan Province, China, had been sequenced on Illumina HiSeq platform with 16523 bp in length, which included 37 genes encoding 13 proteins-coding genes (PCGs), 22 tRNAs, 2 rRNAs and a displacement loop region. The proportion of nucleotides in mitochondrial genome was T (28.7%), C (23.2%), A (32.3%), G (15.8%) with an AT bias of 61%. Maximum-Likelihood phylogenetic tree was reconstructed using concatenated mitochondrial protein-coding genes of
D. roseus
and other 12 fishes. The result of phylogenetic analysis supported the consanguineous relationship among
D. roseus
,
D. rerio, D. nigrofasciatus
and
D. dangila
.
Rhinogobius wuyanlingensis
is endemic to the upper reaches of Feiyunjiang river basin. In this article, the complete mitochondrial genome (mtDNA) for this freshwater goby was first determined. Sequence analysis showed the genome consisted of 13 protein-coding genes, 22 tRNA genes, two ribosomal RNA genes, and two main non-coding regions (the control region and the origin of the light strand replication). This circular molecule was 16,491 bp in length with a slight AT bias of 53.5%. In the phylogenetic tree,
R. wuyanlingensis
was closer to
Rhinogobius brunneus
,
Rhinogobius yonezawai, Rhinogobius flumineus
, and
Rhinogobius cliffordpopei
. The mitochondrial genome of
R. wuyanlingensis
reported here would provide basal molecular data for evolution, taxonomy, and population genetics of
Rhinogobius
.
This study sequenced and annotated the complete mitochondrial genome sequence of
Rhinogobius filamentosus
, an endemic species in China. The complete mitochondrial genome was 16,510 base pairs long and contained 13 protein-coding genes (PCGs, 11,414 bp), 22 tRNA genes (1,555 bp), two rRNA genes (2,615 bp), two non-coding regions (D-loop: control region displacement loop, 478 bp; OL: origin of L-strand replication, 30 bp). The overall base composition of the genome was estimated to be T (25.4%), C (30.2%), A (27.6%) and G (16.7%), showing an AT bias (53%). Phylogenetic analyses revealed that the genus
Rhinogobius
included two clades. For one clade,
R. filamentosus
clustered with
R. duospilus
, and they formed a sister-group relationship with other ten
Rhinogobius
species. This work would be essential in revealing the evolutionary relationships in Gobionellinae.
Complete mitochondrial genome and phylogenetic analysis of
Devario kakhienensis
, endemic minnow from southwest China, for the first time was presented. It was determined to be a 16,777 bp long circular molecule and the genome organization was consistent with that of Danioninae species published previously. Based on PCGs, the maximum likelihood phylogenetic analysis supported the close genetic relationship between
D. kakhienensis
and
Devario interruptus
. These data would contribute toward the genetic resource enrichment, and provide a valuable framework for future research in completely resolving phylogenetic relationships with the family Danionidae.
Here, we sequenced the complete mitogenome of
Rhinogobius szechuanensis
using the Illumina HiSeq platform and submitted the genome to Genbank with accession number OM617727. Assembly circular mitogenome (16,492 bp) consisted of 54.4% AT content, 13 protein-coding genes, 22 tRNA genes, two rRNA genes, an origin of light-strand replication, and a control region. Phylogenetic analysis supported that
R. szechuanensis
was grouped with
R. rubromaculatus
and clustered with other
Rhinogobius
species. The basal molecular data will be essential for further genetics studies such as evolution, taxonomy, DNA barcoding, and population genetics of
Rhinogobius.
The complete mitochondrial DNA genome of
Devario
interruptus was sequenced on the Illumina HiSeq platform and found to be 16,735 bp and included 37 genes encoding 13 proteins, 22 tRNAs, two rRNAs, and two non-coding regions. The proportion of nucleotides in mitochondrial genome was T (27.9%), C (23.7%), A (33%), G (15.4%), and the deviation of AT was 60.9%. A Maximum-Likelihood phylogenetic tree was reconstructed using the concatenated mitochondrial protein-coding genes of
D. interruptus
and other 18 species of fishes. Phylogenetic analysis results supported that
D. interruptus
was closely related to
Devario shanensis
. Fundamental genetic data of
D. interruptus
will be essential for further genetic studies.
Cyprinid fish
Barilius barila
found in the Irrawaddy water system is a valuable fishery resource and has been listed as Least Concern by the IUCN. This study determined the complete mitochondrial genome of
B. barila
from Yunnan, China, for the first time. Circular molecule of
B. barila
mitogenome was sequenced to be 16,560 bp in length, with the typical gene structure of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and two noncoding areas (control region and the origin of L-strand replication). Overall nucleotides composition appeared to be 27.5% A, 24.8% T, 19.2% G, and 28.6% C, with a slight AT (52.3%) bias. The topology of the phylogenetic tree showed that
B. barila
was well grouped with
Opsarius caudiocellatus,
and clustered together with the genus
Opsarius
instead of
Barilius,
revealing that it was more reasonable for
Barilius barila
to belong to
Opsarius
rather than
Barilius
.
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