Modern threats of bioterrorism force the need to develop methods for rapid and accurate identification of dangerous biological agents. Currently, there are many types of methods used in this field of studies that are based on immunological or genetic techniques, or constitute a combination of both methods (immuno-genetic). There are also methods that have been developed on the basis of physical and chemical properties of the analytes. Each group of these analytical assays can be further divided into conventional methods (e.g. simple antigen-antibody reactions, classical PCR, real-time PCR), and modern technologies (e.g. microarray technology, aptamers, phosphors, etc.). Nanodiagnostics constitute another group of methods that utilize the objects at a nanoscale (below 100 nm). There are also integrated and automated diagnostic systems, which combine different methods and allow simultaneous sampling, extraction of genetic material and detection and identification of the analyte using genetic, as well as immunological techniques.

Recently, progress has been achieved in inhalation anthrax treatment. The most promising new possibilities include: new antibiotics, peptides and bacteriophages enzymes, monoclonal antibodies, antigen PA mutants, and inter alpha inhibitors applications. In the case of the possibility of bioterrorist attacks, the examination of inhalation anthrax treatment should be intensively continued.

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Praca obejmuje podsumowanie działalności Ośrodka Diagnostyki i Zwalczania Zagrożeń Biologicznych od jego powstania do chwili obecnej. W szczególny sposób uwzględniono unikalny wkład ośrodka dla Sił Zbrojnych RP wynikający z synergii aktywności: badawczo-rozwojowej, doradczo-eksperckiej oraz diagnostyki klinicznej i środowiskowej w obszarze czynników biologicznych broni masowego rażenia. W ciągu 20 lat w ODiZZB skumulowano wysokiej klasy specjalistów dysponujących wiedzą, umiejętnościami, kompetencjami i doświadczeniem oraz unikalną infrastrukturę badawczą, co pozwoliło nie tylko być istotnym zapleczem dla wojskowej służby zdrowia czy obrony przed bronią masowego rażenia, ale także realizować istotne prace naukowe lub doradcze na rzecz partnerów krajowych lub zagranicznych.

Male SAS/4 mice were injected i.v. with 6.6 kBq 239Pu-citrate. After 1 or 24 h a single i.p. injection of 15 or 30 mumol kg-1 or repeated (three or four) daily injections of 30 mumol kg-1 of tetra-THB-spermine were given, and at 4 or 7 days Pu retention was measured in liver, kidneys and femur. Besides tetra-THB-spermine, equimolar doses of tetra-DHB-spermine were injected for comparison, or equimolar doses of diethylene triamine-pentaacetic acid (DTPA) as a reference compound. Histological changes in kidneys and liver were examined after i.p. injections of 30 mumol kg-1 or at 2-13 times higher doses of tetra-THB-spermine. The results show that: (1) Introduction of an additional hydroxy group into the aromatic moieties of tetra-DHB-spermine results in increased hydrophilicity, lower toxicity and a lower renal retention of Pu. (2) Tetra-THB-spermine and tetra-DHB-spermine are similarly effective in removing plutonium from liver and bone. Their efficacies in removing Pu from bone are approximately similar to those of DTPA but for whole-body removal they are generally inferior. (3) Multiple (30 mumol kg-1) of tetra-THB-spermine were no more effective than a single injection at mobilizing Pu from the liver. (4) Four injections of tetra-THB-spermine induced cloudy swelling and fatty degeneration in epithelial cells of the proximal convoluted tubules. At levels of 400 mumol kg-1 tetra-THB-spermine produced severe degenerative glomerular lesions, foci of liver necrosis and thromboses of the portal vein branch.

Józef Feliks Kubica - higienist and ummunologist. Born 01.01.1928, dead 23.07.2018. During the 2th World War in unit of partisan. Graduated from Warsaw University Department of Biology – 1952. After graduated as as a manager of laboratory in 34 Military Center Statin of Sanitary and Hygiene till 1960. Next manager Department of Immunology in Military Institute of Hygiene and Epidemiology (till 1978). At the same time worked as a senior assistant in Medical University and next manager Laboratory Diagnostic of Syphilis. From 1960-1969 deputy commandant Military Institute of Hygiene and Epidemiology and next twice commanadant MIHE 1969-1978 and 1985-1991. In the mean time was Chief of Group for Research and Developing in Logistical Headquarters PA. Doctorate 1961, reader 1967, associate profesor 1971, profesor 1978. Author over 112 texts of research microbiology, immunology and ecology. Author and co-author a few monographs like a „Immunofluorescence” (PZWL 1967). Retired 03.09.1991 as colonel and still worked in MIHE as a professor. For two tenures Pressident of Science Council MIHE. For many tenures President Committe Ecology of Human PAN.