Сomprehensive assessment of barley plants regenerated from resistant to cadmium callus lines

Широких, И. Г.; Shupletsova, O. N.; Tovstik, E.V.; Ogorodnikova, S. Yu.; Nazarova, Yа. I.; Berezin, G.I.

doi:10.30766/2072-9081.2018.65.4.19-29

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…3). The obtained data are consistent with the results of other researchers, according to which the number of anthocyanins in tissues under the influence of HM (for example, cadmium) increases in individual barley regenerants [19]. In most barley varieties studied, the maximum anthocyanin content in plant leaves was observed at an element concentration of 90 mg/l.…”

Section: Resultssupporting

confidence: 91%

Antioxidant status of barley seedlings under conditions of excess manganese

Simonova¹,

Tovstik²,

Simonov³

2021

E3S Web Conf.

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The system of protection against the effects of heavy metals (TM) can differ not only in different species of plants, but also in different varieties. Manganese (Mn), as a biogenic element, is necessary for normal plant growth and development. However, in the case of concentrations exceeding the optimal level, it negatively affects both linear plant growth and physiological processes. The paper presents data on the assessment of the effect of excess manganese (II) ions (30, 60 and 90 mg/l of active substance) on barley seedlings of different varieties. Resistance of plants to ionic toxicity of manganese (II) was judged by the value of antioxidant activity (AOA) of superoxide dismutase (SOD), the content of low-molecular antioxidants (chlorophyll a and b, carotenoids and anthocyanins) in barley leaves. For most of the varieties studied, an increase in AOA of SOD, the content of anthocyanins indicating the activation of plant protective mechanisms, was noted. The amount of green pigments, as well as carotenoids, on the contrary, decreased, compared with the control, which indicated a violation of the processes of photosynthesis in them.

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Section: Resultssupporting

confidence: 91%

Antioxidant status of barley seedlings under conditions of excess manganese

Simonova¹,

Tovstik²,

Simonov³

2021

E3S Web Conf.

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“…The research objects were barley plants of the line 999-93 and its regenerant forms which were induced by the original genotype in the callus culture in the process of cell selection in the media: 1) with 40 mg•dm -3 Al 3+ (line RAAl); 2) with 15% Polyethylene glycol -PEG as osmoticum (line RAPEG); 3) with 15 mg•dm -3 Cd 2+ (line RAСd) [14,15]. The plantregenerants got in vitro were planted in vegetation vessels with sod-podzolic soil.…”

Section: Methodsmentioning

confidence: 99%

The influence of barley genotype and growing conditions on zinc and cadmium accumulation

Tovstik

Shupletsova

2022

BIO Web Conf.

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Zinc and cadmium accumulation of in plant tissues of barley of the line 999-93 and of its regenerant forms resulting from cell selection was assessed. The scheme of the experiment: 1) control; 2) acidic; 3) cadmium. We used the method of inversion voltammetry to assess the share of zinc and cadmium in plant samples. To assess the share of active and total forms of zinc and cadmium in soil samples collected from root rhizosphere we used the method of atomic absorption spectroscopy. Barley plants accumulated zinc from 0.2 to 79.7 mg∙kg-1 of dry phytomass. The genotype of plants has little influence on zinc accumulation, and plant organs participate in equal measure in zinc accumulation. As for the soil background with cadmium excess, there is a tendency to lessening zinc absorption in all the plant organs, and it is not connected with the plant’s genotype. Unlike zinc, cadmium gets accumulated mostly in the root system. In the control background the share of cadmium in roots was 0.2–0.4 mg∙kg-1; in the acidic one – 0.2–3.6 mg∙kg-1; in the cadmium one – 5.5–9.5 mg∙kg-1. In barley grain grown on soil with excess of cadmium we did not find any IPC excess of cadmium. On backgrounds of the same acidity, the more cadmium concentration grew, the less zinc concentration in grain was, mostly it concerns the original genotype, to a smaller degree it concerns the regenerant line on the selective medium with cadmium and aluminum. Coupling accumulation of zinc and cadmium took place mostly on acidic background, it was characteristic of barley with the original genotype and the regenerant selected in vitro as cadmium-resistant; on control background coupling accumulation is characteristic of aluminum-resistant regenerant. These regenerant genotypes had a tendency to eliminating cadmium and absorbing zinc.

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“…OGORODNIKOVA • YA. I. NAZAROVA Для определения показателей, тестирующих симптомы окислительного стресса, использовали методику, представленную в наших предыдущих исследованиях (Shirokikh et al, 2018). У растений каждого варианта отбирали смешанные пробы листьев (второй подфлаговый лист) в фазе выхода в трубку.…”

Section: материалы и методыunclassified

Effects of nonspecific resistance in barley genotypes obtained by cell selection

Shupletsova

Огородникова

Nazarova

2021

Trudy po prikladnoy botanike, genetike i selektsii

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An effective way to increase the genetic diversity of plants and create sources of resistance to edaphic stressors is the selection of cells under selective in vitro conditions based on somaclonal variation. The use of somaclones (regenerant forms) of barley with resistance to increased acidity, ionic toxicity of aluminum, heavy metals and drought is promising in the northeast of the Non-Black-Soil Zone of Russia. Development of regenerants with integrated resistance to soil stressors due to specific and nonspecific mechanisms is a relevant trend.The target research material was a hybrid combination of spring barley (Luly × Conrad) × 2867-80 and its regenerant forms obtained as a result of cell selection with stressors of various nature. Plants were grown under ordinary soil conditions and on provocative backgrounds with aluminum and cadmium. The productive traits of plants, the symptoms of oxidative stress, and the environment-forming activity of the root system were evaluated.In regenerated plants obtained in callus culture on selective in vitro media with aluminum or water deficiency, an increased ability of the root system to alkalize the medium in the rhizosphere zone (by 0.2–0.5 pH units) when grown on alumina soil was revealed. Against stressful soil backgrounds, regenerative lines induced on in vitro media with cadmium or aluminum were observed to have a low manifestation of oxidative stress symptoms, tested by the intensity of lipid peroxidation and the content of photosynthetic pigments in the leaves, which provided these genotypes with higher seed productivity (1.5–3.6 times in the number of grains, and 1.5– 3.0 times in the grain weight per plant) and adaptive advantages compared to the original genotype and regenerants induced on an in vitro medium with water deficiency. It is concluded that complex resistance to soil aluminum and cadmium toxicity is formed in barley regenerants obtained in the process of cell selection on in vitro media with any of these metals.

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Сomprehensive assessment of barley plants regenerated from resistant to cadmium callus lines

Cited by 4 publications

References 3 publications

Antioxidant status of barley seedlings under conditions of excess manganese

Antioxidant status of barley seedlings under conditions of excess manganese

The influence of barley genotype and growing conditions on zinc and cadmium accumulation

Effects of nonspecific resistance in barley genotypes obtained by cell selection

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