Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenol

Abstract

Gold spherical nanoparticles, gold-platinum nanoflowers, and gold-silver nanostars were obtained and compared as labels for immunochromatographic analysis. The nanoparticles were synthesized by chemical reduction from various precursors and then conjugated with staphylococcal protein A to be used in indirect immunochromatographic determination of nonylphenol. The results obtained were evaluated in terms of analytical characteristics and R2 value, as well as the color intensity of the test band. According to the comparison results, it was revealed that the R2 value varied from 0.82 for the gold-silver nanostars to 0.96 for the spherical gold nanoparticles. The working range of determined concentrations was from 2 to 100 μg/mL for unspherical and from 2 to 50 μg/mL – for spherical markers used; the analysis time was 20 min.

Comparison of Different Formats for Immunochromatographic Detection of Surfactant Nonylphenol

“…Recombinant staphylococcal protein A and soybean trypsin inhibitor (STI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). In the current study, a rabbit antiserum obtained earlier [18,19] was used. The ready-to-use peroxidase substrate solution based on 3,3 ,5,5 -tetramethylbenzidine (TMB) was provided by Immunotech (Moscow, Russia).…”