γ-Pyrone compounds: flavonoids and maltol glucoside derivatives from Herniaria glabra L. collected in the Ternopil region of the Ukraine

Kozachok, Solomiia; Pecio, Łukasz; Kołodziejczyk-Czepas, Joanna; Marchyshyn, S. М.; Nowak, Paweł; Mołdoch, Jarosław; Oleszek, Wiesław

doi:10.1016/j.phytochem.2018.05.009

Cited by 18 publications

(14 citation statements)

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“…There is a large amount of evidence indicating that phenolic compounds are closely associated with, and responsible for antioxidant and antiinflammatory activities [13]. Furthermore, in our previous studies, in vitro single isolates from HG such as narcissin, licoagroside B, apiorutin as well as rutin, exhibited moderate antioxidant activity [14], and phenolic fractions from HIh and HP showed profound antioxidant and anti-inflammatory effects [15]. Thus, the detailed phytochemical characterization and quantification of phenolic metabolites in the purified extracts of HG, HP, and HIh were performed using UHPLC-DAD-QTOF-ESI-HRMS (ultra-high-performance liquid chromatography-diode array detector quadrupole time-of-flight electrospray ionisation high-resolution mass spectrometer) and UPLC-DAD-TQD-MS (TQD, tandem triplequadrupole) respectively.…”

Section: Introductionmentioning

confidence: 81%

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Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

et al. 2022

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The work is aimed at phytochemical characterization and In Vitro evaluation of antioxidant actions, anti-inflammatory effects, and cytotoxicity of purified extracts from three rupturewort (Herniaria L.) species, i.e., Herniaria glabra (HG), H. polygama (HP), and H. incana herb (HIh). The total phenolic content established in the purified extracts (PEs) of HIh, HP, and HG was 29.6, 24.0, and 13.0%, respectively. Thirty-eight non-saponin metabolites were identified using LC-HR-QTOF-ESI-MS; however, only 9 were common for the studied Herniaria species. The most abundant phenolic compound in HG-PE was narcissin (7.4%), HP-PE shared 3 major constituents, namely cis-2-hydroxy-4-methoxycinnamic acid 2-O-β-glucoside (cis-GMCA, 5.8%), narcissin (5.4%), and rutin (5.3%). Almost half of HIh phenolic content (14.7%) belonged to oxytroflavoside A (7-O-methylkaempferol-3-O-[3-hydroxy-3-methylglutaryl-(1→6)]-[α-rhamnopyranosyl-(1®2)]-β-galactopyranoside). Antioxidant properties of the Herniaria PEs were evaluated employing an experimental model of human blood plasma, exposed to the peroxynitrite-induced oxidative stress. The assays demonstrated significant reduction of oxidative damage to protein and lipid plasma components (estimated by measurements of 3-nitrotyrosine, protein thiol groups, thiobarbituric acid-reactive substances), and moderate protection of its non-enzymatic antioxidant capacity. Anti-inflammatory properties of the Herniaria PEs were evaluated In Vitro as inhibitory effects against cyclooxygenases (COX-1 and -2) and concanavalin A-induced inflammatory response of the peripheral blood mononuclear cells (PBMCs). None of the studied plants showed inhibitory effects on COXs but all purified extracts partly reduced the release of interleukin 2 (IL-2) and tumor necrosis factor-alpha (TNF-α) from PBMCs, which suggested their prospective ability to up-regulate inflammatory response of the cells. The purified extract from H. glabra turned out to be the most efficient suppressor of PBMCs’ inflammatory response. Additionally, cytotoxicity of purified Herniaria extracts on PBMCs was ruled out based on In Vitro studies.

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Section: Introductionmentioning

confidence: 81%

“…They are suitable for saponin detection, unlike semi-volatile compounds [17]. As a final result, HG-PE, HP-PE, and HIh-PE contained 82%, 62%, and 52% saponins, respectively [14,15].…”

Section: Phytochemical Profiles and The Content Of Individual Phenoli...mentioning

confidence: 95%

Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

et al. 2022

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“…Afterwards, the PE was fractionated on a Sephadex LH-20 (40–120 μm) glass column (95 cm × 3.2 cm) and connected to a Gilson prep-HPLC (high-performance liquid chromatography) system with ELS™ II detector. The separation was achieved by the flow of acidified 95% MeOH (0.1% formic acid) at a flow rate of 2.4 mL min −1 [49]. Six LH-20 fractions were collected according to the ELS chromatogram, evaporated and freeze-dried to obtain: F1 (0.18 g), F2 (0.17 g), F3 (0.01 g), F4 (0.03 g), F5 (0.06 g), F6 (0.03 g); (Figure S16).…”

Section: Methodsmentioning

confidence: 99%

Phytochemicals of Apple Pomace as Prospect Bio-Fungicide Agents against Mycotoxigenic Fungal Species—In Vitro Experiments

Oleszek

Pecio

Kozachok

et al. 2019

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The phytochemical constituents of apple waste were established as potential antifungal agents against four crops pathogens, specifically, Botrytis sp., Fusarium oxysporum, Petriella setifera, and Neosartorya fischeri. Crude, purified extracts and fractions of apple pomace were tested in vitro to evaluate their antifungal and antioxidant properties. The phytochemical constituents of the tested materials were mainly represented by phloridzin and quercetin derivatives, as well as previously undescribed in apples, monoterpene–pinnatifidanoside D. Its structure was confirmed by 1D- and 2D-nuclear magnetic resonance (NMR) spectroscopic analyses. The fraction containing quercetin pentosides possessed the highest antioxidant activity, while the strongest antifungal activity was exerted by a fraction containing phloridzin. Sugar moieties differentiated the antifungal activity of quercetin glycosides. Quercetin hexosides possessed stronger antifungal activity than quercetin pentosides.

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“…Peripheral blood from healthy volunteers was obtained. The peripheral blood mononuclear cells were separated by Ficoll-Histopaque-1077 density gradient method (Kozachok et al, 2018). The mononuclear cells were washed three times with PBS and then centrifuged at 1250 rpm for 5 min.…”

Section: Isolation Of Peripheral Blood Mononuclear Cells (Pbmcs)mentioning

confidence: 99%

CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.

et al. 2019

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Solanum marginatum L. f. (Solanaceae) is used in traditional Mexican medicine to treat infectious diseases and cancer, although lacks of the scientific validation. Consequently, we proposed a study of the enzymatic, antioxidant, antibacterial and toxic activities of an S. marginatum L. f. protein fraction previously characterized. For this, a protein extract from leaves of S. marginatum L. f. obtained and the protein fraction separated by FPLC. The protein content was quantified by the bicinchoninic acid (BCA) method and the enzymatic activity measured by the Kunitz method. Subsequently, protein subfractions were identified by SDS-PAGE. Additionally, the ability to capture radicals by the DPPH and ABTS methods was determined. The toxic effect was assessed with the A. salina test while cell viability in peripheral blood mononuclear cells (PBMC) tested by MTT method. Isolation of PF-FPLC was carried out at tr = 2.13 min, further, separated and identified seven subfractions with molecular weights ranging from 18-112 kDa, while the optimal conditions of enzymatic activity were 37°C, pH 7 and 120 min. The ABTS and DPPH methods showed a radical capture percentage of 20 and 62% respectively. The PF-FPLC proteins showed no toxicity in A. salina. However, the PF-FPLC showed a low antimicrobial effect (inhibition ≤ 35%), and decreases PBMC viability.

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γ-Pyrone compounds: flavonoids and maltol glucoside derivatives from Herniaria glabra L. collected in the Ternopil region of the Ukraine

Cited by 18 publications

References 59 publications

Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

Phytochemicals of Apple Pomace as Prospect Bio-Fungicide Agents against Mycotoxigenic Fungal Species—In Vitro Experiments

CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.

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