β-estradiol 17-valerate affects embryonic development and sexual differentiation in Japanese medaka (Oryzias latipes)

Lei, Bingli; Kang, Jisoo; Yu, Yingxin; Zha, Jinmiao; Li, Wei; Wang, Zijian

doi:10.1016/j.aquatox.2013.03.011

Cited by 25 publications

(24 citation statements)

References 43 publications

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“…Studies have demonstrated that the VTG and estradiol levels in male or juvenile fish exposed to substituted phenols, including nonylphenol, nitrophenol, and 2,4-dichlorophenol, were significantly increased but were significantly decreased in females (Li et al, 2006b;Ma et al, 2012;Zha et al, 2007). These findings appear to contradict the estrogenic properties of substituted phenols; it seemed that substituted phenols are anti-estrogenic, not estrogenic, in females (Chikae et al, 2004;Lei et al, 2013). Although DCNP is a substituted phenol structurally similar to dichlorophenol and nitrophenol, the disruption of endocrine system by DCNP and its environmental risk to fish are not well documented.…”

Section: Introductioncontrasting

confidence: 49%

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

Chen

Cao

Yuan

et al. 2016

Environmental Pollution

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a b s t r a c t 2,4-Dichloro-6-nitrophenol (DCNP) is an environmental transformation product of 2,4-dichlorophenol that has been identified as widespread in effluent wastewater, but little is known about its toxicity because this compound is not regulated. Therefore, to investigate the endocrine disruption potency of DCNP in Chinese rare minnows (Gobiocypris rarus), adult and juvenile fish were exposed to various concentrations of DCNP (2, 20, and 200 mg/L) for 28 d. After 28 d exposure, the plasma vitellogenin (VTG) levels were reduced in females while increased in males and juvenile fish considerably, as compared with the control. These results suggested that DCNP affects the HPG-axis in a sex-dependent way. Testosterone (T) levels in the plasma were significantly lower in adult and juvenile fish and were accompanied by an increased estradiol (E2)/T ratio. Histopathological observation revealed hypertrophy of the hepatocytes and nuclear pyknosis in the liver, the inhibition of spermatogenesis in the testes, and the degeneration of oocytes in the ovaries after DCNP exposure. The expression pattern of selected genes indicated that the nuclear receptor, steroidogenesis and gonadotropin regulation pathways were perturbed after DCNP exposure. Above all, our results demonstrated that DCNP clearly had anti-androgenic activity in both adult and juvenile fish and can therefore be considered as an endocrine-disrupting chemical.

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Section: Introductioncontrasting

confidence: 49%

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

Chen

Cao

Yuan

et al. 2016

Environmental Pollution

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“…Numerous studies have reported that fish such as medaka (O. latipes), Chinese rare minnow (Gobiocypris rarus), zebra fish (Danio rerio) and guppies (Poecilia reticulata) exposed to estrogens like E2 or EE2 can cause female-biased phenotypic sexratios (Scholz and Gutzeit, 2000;Toft and Baatrup, 2003;Fenske et al, 2005;Zha et al, 2008). In our previous studies, we found that exposure to EV and E1 led to more female medaka fish in genotypic sex-ratios (Lei et al, 2013a;. However, in these two studies, statistical significant analysis for sex ratio between treatments and controls was not performed.…”

Section: Discussionmentioning

confidence: 99%

“…The capacity of E3 to induce feminization of medaka fish was weaker than that of E2, EE2 and EV. These three estrogens at environmental concentrations of 1-10 ng/L and a similar exposure period can result in sex reversal or feminization of male medaka (Caldwell et al, 2012;Lei et al, 2013a). However, this may have some limitations about sensitivities with respect to extrapolating to other medaka populations, because the same brood stock medaka fish in this study have been used for over 5 years.…”

Section: Discussionmentioning

confidence: 99%

“…Nash et al (2004) reported that life-long exposure to 5 ng/L EE2 in the F1 generation of zebrafish (Danio rerio) caused a 56% reduction in fecundity and complete population failure with no fertilization. In our previous work, we also found that β-estradiol 17-valerate (EV) at 1 ng/L can induce feminization and affect normal development of medaka (Oryzias latipes) (Lei et al, 2013a). Several field studies have also shown that these adverse reproduction and development responses in wild fish may be due to chemicals present in aquatic environments (Van Aerle et al, 2001;Jobling et al, 2006;Lange et al, 2011;Yan et al, 2012) and are related to reproductive failure in fish (Nash et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Huang et al (2010) found dose-dependent up-regulation of the Vtg-I gene in the liver of male medaka fish exposed to concentrations of 100 ng/L E2 and above for 2 weeks. In our previous studies, we also found that the levels of ERα and Vtg-I mRNAs in the liver of medaka (O. latipes) exposed to EV and estrone (E1) can be altered (Lei et al, 2013a;. Therefore, Vtg and ER genes have been widely used as marker genes to evaluate the effects of EDCs on fish (Zhang et al, 2008a;Chen et al, 2010;Sellin et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Lei

Kang

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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The growth, development, and ERα and Vtg-I gene expressions of Japanese ricefish (Oryzias latipes; medaka) exposed to different concentrations of estriol (E3), including one environmentally relevant concentration, during embryo-adult life stages were evaluated. At the early life stage, fertilized eggs were exposed to 5, 50, 500, 5000 ng/L E3 for 15 days, and the hatched fry were exposed continuously to the same concentrations for an additional 15 days. Exposure to 500 and 5000 ng/L E3 resulted in adverse effects on hatchability and time to hatching. At 5000 ng/L, the gross abnormality rate was increased and the number of females that hatched was twice that of males. When the fish were exposed to 5-5000 ng/L E3 for further 60 days, the male hepatosomatic index (HSI) was increased at 5000 ng/L. The female gonadosomatic index (GSI) was decreased at 500 and 5000 ng/L E3, while the male GSI at 5000 ng/L E3 was increased and sex reversal was also found at this concentration. Quantitative RT-PCR showed that the hepatic vitellogenin-I (Vtg-I) genes were up-regulated in females at 500 and 5000 ng/L E3 and in males at all E3 concentrations, whereas E3 did not affect estrogen receptor α (ERα) mRNA transcription. These results showed that E3 at environmental concentration of 5 ng/L has no adverse effects on growth and development of the Japanese medaka. However, in this study, if we only focused on Vtg gene change in males, E3 had strong estrogenic effects on male medaka under the conditions of these experiments.

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Reconnaissance Survey of Organic Contaminants of Emerging Concern in the Kabul and Swat Rivers of Pakistan

Schilling Costello,

Asad,

Haris

et al. 2023

Enviro Toxic and Chemistry

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The Swat and Kabul Rivers of Northern Pakistan are within an important regional watershed that support river‐based livelihoods and are impacted by untreated effluent discharges and municipal solid waste. Anecdotal evidence indicates that fish populations are decreasing in these rivers. One potential cause of poor aquatic health is pollution; therefore, we investigated the presence of Contaminants of Emerging Concern (CECs) in the river systems. Water samples were collected in the Kabul River (n=9) and Swat River (n=10) during high (summer 2018) and low (winter 2019) river flow seasons. Agrochemicals, pharmaceuticals, plasticizers, chemicals in personal care products, and hormones were quantified via liquid chromatography high resolution mass spectrometry. In the Swat River, caffeine (18 ng/L to 8452 ng/L), DEET (16 ng/L to 56 ng/L), and plasticizers (13 ng/L to 7379 ng/L) were detected at all sites during both seasons, while butachlor (16 ng/L to 98 ng/L) was detected only during high flow. In the Kabul River, caffeine (12 ng/L to 2081 ng/L) and several plasticizers (91 ng/L to 722 ng/L) were detected at all sites during both seasons, while DEET (up to 97 ng/L) was detected only during high flow. Pharmaceuticals (analgesics and nonsteroidal anti‐inflammatory drugs) were quantified in both rivers (up to 823 ng/L) with detection frequencies from 70‐100% and 0‐78%, respectively during low flow. Intermittent‐use and natural seasonal processes (increased runoff and dilution from rainfall and snowmelt) yielded higher agrochemical concentrations and lower concentrations of continuous‐use compounds (e.g., caffeine) during high flow. This study provides the first insight into CEC concentrations in the Swat River, additional insight into the Kabul River stressors, and overall, contaminant risks to aquatic life.

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β-estradiol 17-valerate affects embryonic development and sexual differentiation in Japanese medaka (Oryzias latipes)

Cited by 25 publications

References 43 publications

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Reconnaissance Survey of Organic Contaminants of Emerging Concern in the Kabul and Swat Rivers of Pakistan

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