α1,3‐Fucoslytransferase IX (Fuc‐TIX) is very highly conserved between human and mouse; molecular cloning, characterization and tissue distribution of human Fuc‐TIX

Abstract

The amino acid sequence of Fuc-TIX is very highly conserved between mouse and human. The number of nonsynonymous nucleotide substitutions of the Fuc-TIX gene between human and mouse was strikingly low, and almost equivalent to that of the K K-actin gene. This indicates that Fuc-TIX is under a strong selective pressure of preservation during evolution. The human Fuc-TIX (hFuc-TIX) showed a unique characteristics, i.e. hFuc-TIX was not activated by Mn 2+ and Co 2+ , whereas hFuc-TIV and hFuc-TVI were activated by the cations. The hFuc-TIX transcripts were abundantly expressed in brain and stomach, and interestingly were detected in spleen and peripheral blood leukocytes.z 1999 Federation of European Biochemical Societies.

Pax6 Controls the Expression of Lewis x Epitope in the Embryonic Forebrain by Regulating α1,3-Fucosyltransferase IX Expression

“…Based on the sequences of mouse and human FucT-IX genes (44,49), oligonucleotides used to amplify the cDNA were as follows: 5Ј-ATGACATCAACATCCAAAGGCATT-3Ј and 5Ј-ACCAA-CAGACTTATATTCTTGATGCC-3Ј. The PCR product was subcloned into the pBluescript SKϪ, and the subcloned fragments were sequenced.…”

“…A series of mammalian ␣3FucT has been cloned to date. In human, six members of the ␣3FucT gene family, FucT-III (35), -IV (36 -38), -V (39), -VI (40,41), -VII (42,43), and -IX (44), have been identified and a subfamily, consisting of FucT-III, -V, and -VI, forms a gene cluster (45). In mouse, a homologue of FucT-III/V/VI has been reported to be a pseudogene (46), while FucT-IV, FucT-VII, and FucT-IX homologues are functional genes (46 -49).…”

“…Each member of the ␣3FucT family differs in its ability to synthesize Lewis x and/or sialyl Lewis x epitopes. While FucT-III, -V, and -VI synthesize both epitopes (39,40), FucT-VII synthesizes only sialyl Lewis x (42,43,48), and FucT-IV and -IX synthesize Lewis x but little or no sialyl Lewis x (37,44,49).…”

Different expression levels of α3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines

“…FUT7 can only synthesize sLe x (9). FUT9 synthesizes Le x (10). FUT10 and FUT11 have been identified from the human genome since they have a conserved ·3 FT domain (11), however their activity has not been validated (12).…”

Sulfated Polysaccharides in Diets for Nile tilapia (Oreochromis niloticus) in the Initial Growth Phase

“…The expression of these genes is responsible for many functions in the organism, such as synthesis of the H blood group antigen [18,19]; synthesis of Lewisx and sialyl-Lewisx antigens [20,21]; addition of fucose to GlcNAc asparagine [22]; and the addition of fucose directly in to polypeptide chains [23]. Thus, this functional property, within the composition of the oligosaccharides, confer to fucose an important role in the health of the immune system.…”