α- and β-cyclodextrins as selective agents for the separation of isomers by reversed-phase high-performance thin-layer and column liquid chromatography

Reversed-phase retention behavior of aromatic compounds involving .beta.-cyclodextrin inclusion complex formation in the mobile phase

“…In practice, numerous studies dealing with the use of an a-or /3-CD and/or its derivative as a mobile phase additive in liquid chromatography have demonstrated that the retention time of a solute that is less hindered for inclusion becomes usually shorter reflecting that the interaction between the solute and the stationary phase is weakened by complex formation (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). Since the change of the retention value caused by the formation of the inclusion complex is closely related to the stability of the inclusion complex, some attempts have been made to estimate the binding or dissociation constant of the complex from the relationship between the retention value and the concentration of CD in the mobile phase (4,9,15,18,21).…”

Solubilization of cyclodextrins for analytical applications

“…The three most commonly employed CDs contain six, seven, and eight such glucose residues and are referred to as -, ß-, and 7-cyclodextrin, respectively. Applications include their use as mobile phase additives in liquid chromatography (1)(2)(3)(4)(5)(6)(7)(8)(9), fluorescence and chemiluminescence enhancement agents (10)(11)(12)(13)(14)(15)(16)(17)(18)26), a medium for the observation of room temperature liquid phosphorescence (19)(20)(21), and reagents in isotachophoresis (22,23), spectrophotometric assays (24), and NMR optical purity measurements (25) among others. In many of these applications, the CD concentration is very important and dictates the maximum analytical response obtainable.…”