Zur kolorimetrischen Milchsäurebestimmung in Silagen mit p-Hydroxydiphenyl

Haacker, Karl; Block, H. J.; Weißbach, Friedrich

doi:10.1080/17450398309425704

Cited by 63 publications

(20 citation statements)

References 4 publications

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“…In vitro OMD was measured according to Friedel (1990). Fresh silage samples were analysed for pH, water-soluble carbohydrates by the method of Somogyi (1945), lactic acid (Haacker et al, 1983), volatile fatty acids (Huhtanen et al, 1998), ammonia N (McCullough, 1967, ethanol with an enzymatic kit (Cat No. 981680, KONE Instruments Corporation, Espoo, Finland) and soluble N by the Kjeldahl method (AOAC, 1990, No.…”

Section: Sampling and Analysismentioning

confidence: 99%

Effects of feeding frequency on the performance and welfare of mature Hereford cows and their progeny

et al. 2006

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Section: Sampling and Analysismentioning

confidence: 99%

Effects of feeding frequency on the performance and welfare of mature Hereford cows and their progeny

et al. 2006

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“…To obtain supernatant for the formic acid analysis, 10 g of pelleted feed were mixed with 290 g of H 2 O, stirred twice for 5 min with 30 min of cooling (at 218 8C) in between, and then centrifuged at 2000 g for 10 min. Lactic acid was analysed according to Haacker et al (1983), short chain fatty acids according to Huhtanen et al (1998), ammonia according to McCullough (1967), and ATP according to Jensen and Jørgensen (1994).…”

Section: Chemical Analysesmentioning

confidence: 99%

Effects of a dietary organic acid mixture and of dietary fibre levels on ileal and faecal nutrient apparent digestibility, bacterial nitrogen flow, microbial metabolite concentrations and rate of passage in the digestive tract of pigs

Partanen

Jalava

Valaja

2007

Animal

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Six 34-kg barrows were fitted with a post-valve T-caecum cannula and assigned to six dietary treatments according to a 6 £ 5 change-over design to study how a mixture of formic acid, sorbate, and benzoate (0 or 8.4 g/kg feed) influences apparent ileal and faecal digestibility coefficients, bacterial nitrogen (N) flow, microbial metabolite concentrations, and passage rate in pigs fed isoenergetic diets with medium, high, or very high fibre content (neutral-detergent fibre (NDF): 199, 224, and 248 g/kg dry matter, respectively). These barley and soya-bean meal based diets contained 0, 75, and 150 g/kg barley fibre (NDF: 577 g/kg) and 0, 8, and 16 g/kg rapeseed oil, respectively. The dietary organic acid mixture improved the apparent ileal digestibility of 14 of the 17 amino acids analysed (P , 0.05). Increasing levels of dietary fibre linearly decreased the apparent ileal digestibility of six of the 17 amino acids analysed (P , 0.05). Ileal flows of bacterial N and amino acids as assessed on the basis of purine flow were decreased by the dietary organic acid mixture (P , 0.05) but were not affected by dietary fibre level (P . 0.05). As assessed on the basis of diaminopimelic acid flow, bacterial N flow was increased by both the dietary organic acid mixture and increased dietary fibre levels (P , 0.05). The dietary organic acid mixture reduced the concentration of lactic acid and increased that of acetic acid in ileal digesta (P , 0.05), while dietary fibre levels had a quadratic effect on concentrations of acetic, propionic, and butyric acid (P , 0.05). The mean retention time of Co (solute marker) and Yb (particle marker) in the large intestine decreased in a linear manner by increasing dietary fibre levels (P , 0.05) but was not affected by the dietary organic acid mixture (P . 0.05). The results show that a dietary organic acid mixture has a positive effect on the apparent ileal digestibility of most amino acids irrespective of dietary fibre levels. This could be at least partly related to changes in bacterial N flow in the ileum. However, different bacterial markers showed opposite effects on bacterial N flow, which makes it questionable to use a constant bacterial marker / bacterial N ratio to estimate bacterial N flow. Increasing levels of dietary fibre had negative effects on the apparent ileal amino acid digestibilities and shortened the mean retention time of digesta in the large intestine.

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“…At the end of the 24-h incubation, the fermentation fluid was sampled to determine its pH and its microbial metabolite concentrations. The fermentation fluid was frozen as such for lactic acid analysis according to Haacker et al (1983). Volatile fatty acids were analysed according to Huhtanen et al (1998), and for the analysis 1 ml of fermentation fluid was first mixed with 0.1 ml of saturated HgCl 2 , then with 0.4 ml of 1 N NaOH, and then frozen.…”

Section: In Vitro Fermentationmentioning

confidence: 99%

Effects of some organic acids and salts on microbial fermentation in the digestive tract of piglets estimated using an in vitro gas production technique

Partanen¹,

Jalava²

2008

AFSci

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An in vitro gas production technique was used to screen different organic acids (formic, propionic, lactic, citric, and fumaric acid), organic salts (calcium formate, potassium sorbate, and sodium benzoate), and inorganic phosphoric acid for their ability to modulate microbial fermentation in the digestive tract of piglets. For the incubation, 40 ml of culture medium (53% buffer, 45% frozen ileal digesta, and 2% fresh faeces) was dispensed in vessels containing 5 ml of buffer, 0.5 g of feed, and 20 µl of liquid or 20 mg of solid acidifiers. Gas production was measured every 15 min during the 24 h incubation at 39°C, and a Gompertz bacterial growth model was applied to the gas production data. Formic acid was the only acid that reduced the maximum rate of gas production (µ m ) compared to that in the control treatment (P < 0.05). The µ m was slower in vessels with formic acid than in those with calcium formate, citric acid, and potassium sorbate (P < 0.05). Calcium formate increased the µm compared to the control treatment (P < 0.05). The maximum volume of gas produced and the lag time did not differ between different acidifiers (P > 0.05). When investigating formic-acid-based mixtures that contained 1-5% of potassium sorbate and/or sodium benzoate, the estimated parameters for the Gompertz growth model did not differ from those for treatments with plain formic acid (P > 0.05). However, concentrations of total volatile fatty acids, acetic acid, propionic acid, and n-butyric acid were reduced by all the mixtures (P < 0.05), but not by plain formic acid (P > 0.05). In conclusion, organic acids and salts were found to differ in their ability to modulate microbial fermentation in the digestive tract of piglets. Mixing formic acid with potassium sorbate or sodium benzoate changed fermentation patterns, and the possibility to use them to enhance the antimicrobial effect of formic acid should be investigated further in vivo.

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Zur kolorimetrischen Milchsäurebestimmung in Silagen mit p-Hydroxydiphenyl

Cited by 63 publications

References 4 publications

Effects of feeding frequency on the performance and welfare of mature Hereford cows and their progeny

Effects of feeding frequency on the performance and welfare of mature Hereford cows and their progeny

Effects of a dietary organic acid mixture and of dietary fibre levels on ileal and faecal nutrient apparent digestibility, bacterial nitrogen flow, microbial metabolite concentrations and rate of passage in the digestive tract of pigs

Effects of some organic acids and salts on microbial fermentation in the digestive tract of piglets estimated using an in vitro gas production technique

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