Zunyimycins B and C, New Chloroanthrabenzoxocinones Antibiotics against Methicillin-Resistant Staphylococcus aureus and Enterococci from Streptomyces sp. FJS31-2

Abstract

This study performed an optimization of the fermentation conditions to activate the expression of the zunyimycin family biosynthesis genes of the zunyimycin-producing streptomycetes strain Streptomyces sp. FJS31-2. Bioassay-guided isolation and purification by varied chromatographic methods yielded two new compounds of the zunyimycin derivatives, namely, 31-2-7 and 31-2-8, accompanied with three known anthrabenzoxocinones family members of zunyimycin A, BE24566B, and chloroanthrabenzoxocinone. Their structures were elucidated by NMR, HRESIMS, IR, UV, and CD. Results showed that these two compounds were structurally similar to the previously reported compound zunyimycin A but differed in positions and number of chlorine atom substitution. The two novel compounds were called zunyimycins B and C. Antibacterial activity assay indicated that zunyimycin C showed a good inhibitory effect on the methicillin-resistant Staphylococcus aureus and Enterococci.

Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)

“…FJS31-2 indicated that its genome contains a gene cluster that is responsible for production of anthrabenzoxocinones with potential antibacterial activities, previously known BE-24566B and newly identified zunyimycin A [29]. The same Streptomyces strain, when cultured under various laboratory conditions, produced other compounds from this group, named zunyimycins B and C, inhibiting growth of both MRSA and enterococci [30]. These results demonstrated a high potential of Actinobacteria to produce different antimicrobial compounds that may be modified under different environmental conditions.…”

Promising bioactive compounds from the marine environment and their potential effects on various diseases

“…Zunyimycins B and C isolated from the Streptomyces sp. FJS31-2 exhibited antimicrobial activity with MIC = 0.94 μg/ml and MICs between 3.75–8.14 μg/ml against MRSA isolates [ 22 ].…”

“… More significant cytotoxicity and antimicrobial activity due to the vinyl side chain 102439806 [ 21 ] 43 Zunyimycins Streptomyces sp. FJS31-2 Inhibits the proliferation of lung cancer cells by the activation of apoptosis by an AKT pathway [ 22 ] 44 Formicamycins A Streptomyces formicae Potent antibacterial activity against clinical MRSA and VRE isolates [ 23 ] 45 Allocyclinones Actinoallomurus Possess activity against various Gram-positive bacteria, including antibiotic-resistant strains, with increasing antibacterial potency with the number of chlorine substitutes [ 24 ] 46 Ageloline A Streptomyces sp. SBT345 Able to reduce oxidative stress and genomic damage induced by the oxidative stress inducer 4-nitroquinoline-1-oxide (NQO) 1884 [ 26 ] 47 Buanmycin Streptomyces sp Inhibition of sortase A, which is a promising target for antibiotic Discovery [ 89 ] 48 Citreamicin Streptomyces caelestis Citreamicins exhibit cytotoxic activity against HeLa and Hep62 cells in addition to their potent antibiotic activity 3083114 [ 28 ] 49 Kocurin Kocuria sp.…”

Secondary Metabolites from Streptomyces sp CSDX076 for the First Time

“…Previously reported chemical investigations of the genus Streptomyces include the antifungal hygrolidin (7), cytotoxic elmonin (8), antibacterial angumycinone C (9) and antitumour streptonoate (10). Additionally, it had been implied that the genus Streptomyces was a good source of antibiotic (11)(12)(13). In our previous research, we conducted the isolation, and activity screening of the strains from the soil at different altitudes in Chishuidanxia city and Fanjinshan mountain, Guizhou Province, People's Republic of China (PRC).…”