Zeta potential change of Neuro-2a tumor cells after exposure to alumina nanoparticles

Abstract

Abstract. In recent years, researches have paid much attention to the physical, chemical, biophysical and biochemical properties of a cell surface. It is known that most of the cells' surfaces are charged. This charge depends on the biochemical structure of the cell membranes. Therefore, measurement of a cell surface charge is a significant criterion that gives information about the cell surface. Evaluation of the cells zeta-potential is important to understand the interaction mechanisms of various drugs, antibiotics, as well as the interaction of nanoparticles with the cell surface. In this study, we use the dynamic light scattering method to detect the zeta-potential change of Neuro-2a tumor cells. It has been observed that zeta-potential shifted to negative values after exposure to metal oxide nanoparticles and inducing apoptosis.

Inhibition of Autophagy Increases Cell Death in HeLa Cells through Usnic Acid Isolated from Lichens

“…A shift in the zeta potential towards the more negative side may indicate the exposure of negatively charged phospholipids, especially phosphatidylserine, to the outer membrane, which occurs during apoptosis. Kazantsev et al [ 42 ] correlated a similar decrease in the zeta potential of neuro-2a tumor cells with cytotoxicity after their exposure to alumina nanoparticles.…”

Biomedical applications of aluminium oxide nanoparticles

“…Among the petal, plate and wedge-like nano-aluminium oxide, the nanopetal aluminium oxide showed the highest level of cell cytotoxicity, followed by the nanoplates and wedge-like NPs. Also, the highest change in the zeta potential of cell surface has been observed for AlNPs [20]. As an advanced strategy for cancer treatment, AlNPs have been applied as efficient adjuvant in cancer immunotherapy in vitro and in vivo.…”

“…Also, with the help of other disciplines such as nanoscale surface chemistry, AlNPs can be used as artificial enzymes for developing novel diagnostic assays. [14] and ibuprofen [15] biosensing bovine serum albumin [16], DNA in a competitive bioassay [17], hydroquinone [18] cancer therapy anti-cancer therapy [19], cytotoxic agents to induce cell death in human prostate cancer cells [9], changing the zeta potential of cell surface [20], boosting the efficacy of cancer vaccines [21] anti-microbial effects strong anti-microbial activities [2], potential antimicrobial properties against E. coli and S. epidermidis [7], photothermal killing effects [22] treatment of other diseases anti-asthmatic [23], as nano-thrombolytic system [24] bimolecular preservation as nanoplatform for correct refolding of mis-/un-folded protein molecules [25] immunotherapy as leishmania vaccine to induce autophagy in macrophages [26], as potent vaccination adjuvant [27]…”