Zebrafish fin regeneration after cryoinjury-induced tissue damage

Abstract

Although fin regeneration following an amputation procedure has been well characterized, little is known about the impact of prolonged tissue damage on the execution of the regenerative programme in the zebrafish appendages. To induce histolytic processes in the caudal fin, we developed a new cryolesion model that combines the detrimental effects of freezing/thawing and ischemia. In contrast to the common transection model, the damaged part of the fin was spontaneously shed within two days after cryoinjury. The remaining stump contained a distorted margin with a mixture of dead material and healthy cells that concomitantly induced two opposing processes of tissue debris degradation and cellular proliferation, respectively. Between two and seven days after cryoinjury, this reparative/proliferative phase was morphologically featured by displaced fragments of broken bones. A blastemal marker msxB was induced in the intact mesenchyme below the damaged stump margin. Live imaging of epithelial and osteoblastic transgenic reporter lines revealed that the tissue-specific regenerative programmes were initiated after the clearance of damaged material. Despite histolytic perturbation during the first week after cryoinjury, the fin regeneration resumed and was completed without further alteration in comparison to the simple amputation model. This model reveals the powerful ability of the zebrafish to restore the original appendage architecture after the extended histolysis of the stump.

Vegfc/d-dependent regulation of the lymphatic vasculature during cardiac regeneration is influenced by injury context

“…For these experiments we chose the fin, as both resection and cryoinjury also result in regeneration of this tissue. 41,42 Consistent with the response of the heart, cryoinjury of the caudal fin stimulated an organ-wide activation of the lymphatic reporter transgene whereas amputation of this appendage failed to elicit any lymphatic vascular outgrowth (Supplementary Fig. 5).…”

Zebrafish as an Emerging Model for Osteoporosis: A Primary Testing Platform for Screening New Osteo-Active Compounds

“…These juvenile osteoblasts then secrete matrix with intermediate properties between cartilage and bone and are later remodeled as mature bone by matured osteoblasts and recruited osteoclasts (Figure 4B) (33, 128). These fins can also be injured via cryo-injury by placing a −196°C knife perpendicularly to the caudal fin rays allowing to study the bone resorption response (130). These techniques offer great perspectives to compare bone formation and bone remodeling, in an adult context.…”

Antrodia cinnamomea Extraction Waste Supplementation Promotes Thermal Stress Tolerance and Tissue Regeneration Ability of Zebrafish

“…The zebrafish fin provides a valuable model to assess the organ regeneration ability of fish [ 33 , 34 ]. Regeneration is the process by which damaged or lost structures are perfectly or near-perfectly replaced.…”