Zebrafish as a Vertebrate Model to Assess Sublethal Effects and Health Risks of Emerging Pollutants

Raldúa, Demetrio; Barata, Carlos; Casado, Marta; Faria, Melissa; Navas, José María; Olivares, Alba; Oliveira, Eva; Pelayo, Sergi; Thienpont, Benedicte; Piña, Benjamı́n

doi:10.1007/698_2011_124

Cited by 1 publication

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“…The ZET is not considered an animal test as long as the zebrafish embryos in the test are not free-feeding, which is typically up to 120 h post fertilization (hpf) (EU, 2010;Sellick, 2011). Comparing to the other in vitro systems for developmental toxicity testing, such as the mEST which is based on a cell line, the ZET utilizes intact organisms in a simple and fast culture system and covers the whole vulnerable developmental stages of the zebrafish embryos (Raldúa et al, 2012;. This makes the ZET a promising model for evaluating the adverse effects of chemicals and substances.…”

Section: Zebrafish Embryotoxicity Test (Zet)mentioning

confidence: 99%

Use of new approach methodologies to study the developmental toxicity of polycyclic aromatic hydrocarbons

Fang¹

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Background and aim of the thesisPolycyclic aromatic hydrocarbons (PAHs) make up a large group of organic compounds with thousands of individual chemicals (EFSA, 2008). Their molecular structure contains at least three benzene rings (with only carbon and hydrogen atoms) in linear, angular or clustered arrangements . PAHs are derived from natural-biological or anthropogenic sources . The natural-biological PAHs are generated by plants, algae and microorganism, and are also derived from oil seeps, early diagenesis, forest fires, and volcanoes (Mojiri et al., 2019;. The anthropogenic sources of PAHs are either petrogenic or pyrogenic. Most pyrogenic PAHs are generated by pyrolysis or incomplete combustion of organic matters such as coal, petroleum and wood (Stout et al., 2015). Petrogenic PAHs derive mainly from crude oil and petroleum products such as gasoline, petroleum gas, kerosene, diesel, heavy fuel oil, gas oil, distillate aromatic extract, lubricating base oil, asphalt and paraffin wax . Both pyrogenic and petrogenic PAHs are highly complex materials and their chemical composition can vary substantially.Unsubstituted PAHs are abundant in pyrogenic substances while alkylated PAHs (e.g., monomethylated, dimethylated and ethylated PAHs) account for a relatively large proportion of the PAHs present in the petrogenic substances Sun et al., 2014). This implies that the PAHs present in crude oil and its refined products i.e., petroleum substances, are dominated by alkylated PAHs instead of their unsubstituted/parent PAHs. It is of interest to note that while numerous studies focus on the toxicity of unsubstituted PAHs, limited is known about the toxicity of the alkylated PAHs. However, it was reported that the methylated congeners of some PAHs could induce either higher or lower toxicity compared to their corresponding unsubstituted/parent PAHs . Thus, it can be hypothesized that the position of the methyl substituent on the aromatic ring of PAHs could either increase or decrease their toxicity such as for example their developmental toxicity.Regarding petroleum substances, several in vivo studies have reported that the observed developmental toxicity (e.g., increased resorptions and reduced fetal body weights in rats) of petroleum substances might be related to certain polycyclic aromatic hydrocarbons (PACs) known to be present in some petroleum substances i.e., distillate aromatic extract and clarified slurry oil (Feuston et al., 1997(Feuston et al., , 1989. PACs present in petroleum substances consists of naked/unsubstituted or alkylated PAHs and heterocyclic aromatics (Carrillo et al., 2019;. Whereas for petroleum substances that are devoid of confounding effects of other hazardous chemicals (IARC, 2010; Kennish, 2016). Thus, the definite developmental effects of PAHs, especially individual PAHs including substituted PAHs, on fetuses/embryos upon prenatal exposure remain to be fully elucidated.To date, developmental toxicity studies using experimental animals are mostly limited to studies on the unsubstituted PA...

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Section: Zebrafish Embryotoxicity Test (Zet)mentioning

confidence: 99%

Use of new approach methodologies to study the developmental toxicity of polycyclic aromatic hydrocarbons

Fang¹

View full text Add to dashboard Cite

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Zebrafish as a Vertebrate Model to Assess Sublethal Effects and Health Risks of Emerging Pollutants

Cited by 1 publication

References 73 publications

Use of new approach methodologies to study the developmental toxicity of polycyclic aromatic hydrocarbons

Use of new approach methodologies to study the developmental toxicity of polycyclic aromatic hydrocarbons

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