Ypt11 Functions in Bud-Directed Transport of the Golgi by Linking Myo2 to the Coatomer Subunit Ret2

Arai, Seisuke; Noda, Yoichi; Kainuma, Satoko; Wada, Ikuo; Yoda, Koji

doi:10.1016/j.cub.2008.06.028

Cited by 50 publications

(44 citation statements)

References 15 publications

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“…It is reasonable to speculate that other Golgi-derived vesicles are transported along astral microtubules by motor dynein. In budding yeast, the actin cytoskeleton plays an essential role in Golgi transport during mitosis (Arai et al, 2008). We failed to observe co-localization of COPI vesicles with actin networks in male meiotic cells (D.K.…”

Section: Dependence Of Copi Vesicle Distribution On Astral Microtubulmentioning

confidence: 83%

COPI-mediated membrane trafficking is required for cytokinesis inDrosophilamale meiotic divisions

Kitazawa

Yamaguchi

Mori

et al. 2012

Journal of Cell Science

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SummaryThe coatomer protein complex, COPI, mediates retrograde vesicle transport from the Golgi apparatus to the ER. Here, we investigated the meiotic phenotype of Drosophila melanogaster spermatocytes expressing dsRNA of 52 genes encoding membrane-traffickingrelated factors. We identified COPI as an essential factor for male meiosis. In Drosophila male meiotic divisions, COPI is localized in the ER-Golgi intermediate compartment of tER-Golgi units scattered throughout the spermatocyte cytoplasm. Prior to chromosome segregation, the vesicles assemble at the spindle pole periphery through a poleward movement, mediated by minus-end motor dynein along astral microtubules. At the end of each meiotic division, COPI-containing vesicles are equally partitioned between two daughter cells. Our present data strongly suggest that spermatocytes possess a regulatory mechanism for equal inheritance of several types of membrane vesicles. Using testis-specific knockdown of COPI subunits or the small GTPase Arf or mutations of the cCOP gene, we examined the role of COPI in male meiosis. COPI depletion resulted in the failure of cytokinesis, through disrupted accumulation of essential proteins and lipid components at the cleavage furrow region. Furthermore, it caused a reduction in the number of overlapping central spindle microtubules, which are essential for cytokinesis. Drosophila spermatocytes construct ER-based intracellular structures associated with astral and spindle microtubules. COPI depletion resulted in severe disruption of these ER-based structures. Thus, we propose that COPI plays an important role in Drosophila male meiosis, not only through vesicle transport to the cleavage furrow region, but also through the formation of ER-based structures.

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Section: Dependence Of Copi Vesicle Distribution On Astral Microtubulmentioning

confidence: 83%

COPI-mediated membrane trafficking is required for cytokinesis inDrosophilamale meiotic divisions

Kitazawa

Yamaguchi

Mori

et al. 2012

Journal of Cell Science

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“…Late-Golgi elements are moved by Myo2p with Ypt11p, a Rab GTPase, and Ret2p, a subunit of the coatomer complex (Rossanese et al, 2001;Arai et al, 2008). Secretory vesicles are moved by Myo2p (Pruyne et al, 1998;Schott et al, 1999) and require the Rab GTPases Ypt31p and Ypt32p (Casavola et al, 2008;Lipatova et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

PTC1Is Required for Vacuole Inheritance and Promotes the Association of the Myosin-V Vacuole-specific Receptor Complex

Jin

Eves

Tang

et al. 2009

MBoC

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Organelle inheritance occurs during cell division. In Saccharomyces cerevisiae, inheritance of the vacuole, and the distribution of mitochondria and cortical endoplasmic reticulum are regulated by Ptc1p, a type 2C protein phosphatase. Here we show that PTC1/VAC10 controls the distribution of additional cargoes moved by a myosin-V motor. These include peroxisomes, secretory vesicles, cargoes of Myo2p, and ASH1 mRNA, a cargo of Myo4p. We find that Ptc1p is required for the proper distribution of both Myo2p and Myo4p. Surprisingly, PTC1 is also required to maintain the steady-state levels of organelle-specific receptors, including Vac17p, Inp2p, and Mmr1p, which attach Myo2p to the vacuole, peroxisomes, and mitochondria, respectively. Furthermore, Vac17p fused to the cargo-binding domain of Myo2p suppressed the vacuole inheritance defect in ptc1⌬ cells. These findings suggest that PTC1 promotes the association of myosin-V with its organelle-specific adaptor proteins. Moreover, these observations suggest that despite the existence of organelle-specific receptors, there is a higher order regulation that coordinates the movement of diverse cellular components. INTRODUCTIONDuring each cell cycle, cytoplasmic organelles are actively distributed between dividing cells to maintain organelle copy number and volume. The yeast Saccharomyces cerevisiae is an excellent model system for studying the spatial and temporal control of organelle inheritance. In yeast, several organelles are transmitted from mother to daughter cells. These include the vacuole, mitochondria, the endoplasmic reticulum (ER), late-Golgi elements and peroxisomes (reviewed in Weisman, 2006).Early in the cell cycle, a portion of each organelle is transported into the emerging bud. The polarized transport of most organelles from the mother to the bud is an active process that requires the actin cytoskeleton, myosin-V motors, and receptor proteins, which physically connect the motor to organelle cargoes (Beach et al., 2000;Yin et al., 2000;Boldogh et al., 2001;Barr, 2002;Bretscher, 2003;Du et al., 2004;Fagarasanu et al., 2006bFagarasanu et al., , 2007Weisman, 2006). Thus, formation of a complex between the motor and receptor protein is important for polarized organelle transport.Similar to yeast, in vertebrates, myosin-V motors move cargoes along the actin cytoskeleton. The best studied cargo of vertebrate myosin-V are melanosomes, which are moved in melanocytes by myosin-Va. Melanosomes attach to myosin-Va through Rab27a and melanophilin (Fukuda and Kuroda, 2002;Wu et al., 2002). Rab27a, through geranylgeranylation, attaches to the melanosome membrane, and melanophilin connects myosin-Va and Rab27a. Myosin-V-based intracellular movement has been analyzed in many other eukaryotes, including frogs, fish, mammals, and plants; plant myosin-XI is the functional homologue of yeast and vertebrate myosin-V ( Kinkema and Schiefelbein, 1994;Provance and Mercer, 1999;Tuma and Gelfand, 1999;Desnos et al., 2007;Li and Nebenfuhr, 2007;Sheets et al., 2007;Shimmen, 2007).Ge...

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“…in figure). Ypt11p has been identified as an adaptor protein that connects late Golgi cisternae to Myo2p through its interaction with the coatomer subunit Ret2p (Arai et al, 2008), as an adaptor for Myo2p on mitochondria (Itoh et al, 2002), as required for the retention of newly inherited mitochondria at the bud tip (Boldogh et al, 2004) and as a docking protein for cER at the bud tip (Buvelot Frei et al, 2006). Ypt11p is a Rab GTPase that is regulated by phosphorylation and degradation (Lewandowska et al, 2013).…”

Section: Box 1 Open Questions In the Field Of Mitochondrial Inheritancementioning

confidence: 99%

“…Of note, although the cER and mitochondria are moved to the bud by two independent motors (Estrada et al, 2003), a defect in ER inheritance has also been shown to impair mitochondrial inheritance. Ypt11p is a Rablike protein that localizes to the cER (Buvelot Frei et al, 2006) and also interacts with Myo2p (Arai et al, 2008;Itoh et al, 2004). Deletion of YPT11 not only prevents the accumulation of cER at the bud tip (Buvelot Frei et al, 2006) but also inhibits the anchoring of mitochondria at the bud tip (Itoh et al, 2002;Swayne et al, 2011).…”

Section: Commentarymentioning

confidence: 99%

“…Both proteins bind Myo2p directly and have been implicated in mitochondrial inheritance, as small buds of mmr1D and ypt11D cells lack mitochondria (Frederick et al, 2008;Itoh et al, 2004). However, the localization of Ypt11p to the ER (Buvelot Frei et al, 2006) and late Golgi vesicles (Arai et al, 2008) raises the question as to whether Ypt11p attaches mitochondria directly to Myo2p or moves ER elements to which mitochondria adhere (see also Box 1). Adaptor proteins that attach Myo2p to secretory vesicles include the Rab GTPases Ypt31p, its paralog Ypt32p and Sec4p, as well as the exocyst component Sec15p, which mediates the polarized delivery of transport vesicles to the plasma membrane (Jin et al, 2011;Lipatova et al, 2008;Santiago-Tirado et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Sharing the cell's bounty – organelle inheritance in yeast

Knoblach

Rachubinski

2015

Journal of Cell Science

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Eukaryotic cells replicate and partition their organelles between the mother cell and the daughter cell at cytokinesis. Polarized cells, notably the budding yeast Saccharomyces cerevisiae, are well suited for the study of organelle inheritance, as they facilitate an experimental dissection of organelle transport and retention processes. Much progress has been made in defining the molecular players involved in organelle partitioning in yeast. Each organelle uses a distinct set of factors -motor, anchor and adaptor proteins -that ensures its inheritance by future generations of cells. We propose that all organelles, regardless of origin or copy number, are partitioned by the same fundamental mechanism involving division and segregation. Thus, the mother cell keeps, and the daughter cell receives, their fair and equitable share of organelles. This mechanism of partitioning moreover facilitates the segregation of organelle fragments that are not functionally equivalent. In this Commentary, we describe how this principle of organelle population control affects peroxisomes and other organelles, and outline its implications for yeast life span and rejuvenation.

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Ypt11 Functions in Bud-Directed Transport of the Golgi by Linking Myo2 to the Coatomer Subunit Ret2

Cited by 50 publications

References 15 publications

COPI-mediated membrane trafficking is required for cytokinesis inDrosophilamale meiotic divisions

COPI-mediated membrane trafficking is required for cytokinesis inDrosophilamale meiotic divisions

PTC1Is Required for Vacuole Inheritance and Promotes the Association of the Myosin-V Vacuole-specific Receptor Complex

Sharing the cell's bounty – organelle inheritance in yeast

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