Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro

Abstract

The effect of dietary administered young barley containing a mixture of phytochemicals to female rats for the prevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis was evaluated. After carcinogen administration (14 wk), mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation of possible mechanisms in MCF-7 breast cancer cell line was performed. Barley (0.3%) demonstrated mild antitumor effect in mammary carcinogenesis, yet 3% barley did not further improve this effect. Immunohistochemical analysis of rat tumor cells in treated groups showed significant increase in caspase-3 expression and significant reduction in Ki67 expression. In addition, 3% barley significantly decreased dityrosine levels versus control. Barley in higher dose significantly decreased serum low-density lipoprotein-cholesterol in rats. In vitro studies showed that barley significantly decreased survival of MCF-7 cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and significantly decreased 5-bromo-20-deoxyuridine incorporation versus control. Barley prevented cell cycle progression and extended incubation with barley showed significant increase in the percentage of annexin V/propidium iodide-positive MCF-7 cells. Our results propose an antitumor effect for the mixture of phytochemicals present in young barley in a breast cancer model.

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

“…The expression of Ki67 is rigorously associated with the cell cycle progression . In this study, the decrease in Ki67 expression in rat mammary carcinomas is in accordance with our earlier results testing different plant functional foods . Our in vitro study (MTS and BrdU assays and analysis of cell cycle) confirmed antiproliferative effects of CLOs in breast adenocarcinoma cells.…”

“…Free radicals that damage important cell macromolecules play a substantial role in the etiopathogenesis of cancer . Recently, we have found that dietary administered young barley, rich in antioxidants (flavonoids), apparently lowered the levels of dityrosines in mammary cancer cells in rats . MDA is often used as a marker of the oxidative damage of lipids by free radicals .…”

Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549

“…The inhibition of cancer cell cycle progression is very often described as one of the mechanisms of anticancer action. Such an activity of young barley was described by Kubatka in MCF-7 breast cancer cells [27]. In the presented study, no significant changes were observed in the HT-29 and A549 cells cell cycle progression after exposure to the HWE extract (Tab.…”

“…Recently, the antiproliferative potential of young barley extracts has been revealed in breast cancer cells [27]. In the study by Kubatka et al, the tested barley extract at the concentration 1 mg/ml decreased MCF-7 breast cancer cells proliferation to 37.93% after 72 h treatment.…”

Fluctuations of Histone Chemical Modifications in Breast, Prostate, and Colorectal Cancer: An Implication of Phytochemicals as Defenders of Chromatin Equilibrium

“…Plant-derived bioactive molecules (phytochemicals) are of particular interest within oncological research. Numerous phytochemicals or natural mixtures of plant compounds present in whole foods show significant antitumor properties via multiple cell signaling pathways and mechanisms, and thus, represent perspective and potentially effective tools for chemoprevention and targeted therapy of cancer disease [14,30,[169][170][171][172]. Moreover, an administration of dietary phytochemicals is the way of a cost-effective and readily applicable clinical approach in the management of cancer, including the most commonly occurring BC, PC, and CRC.…”