Yeast-assisted synthesis of polypyrrole: Quantification and influence on the mechanical properties of the cell wall

Andriukonis, Eivydas; Stirkė, Arūnas; Garbaras, Andrius; Mikoliūnaitė, Lina; Ramanavičienė, Almira; Remeikis, Vidmantas; Thornton, Barry; Ramanavičius, Arūnas

doi:10.1016/j.colsurfb.2018.01.034

Cited by 35 publications

(29 citation statements)

References 53 publications

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“…Notably, the cells coated with PPy exhibited comparable viability to normal cells. Furthermore, polymerization of PPy can also be achieved by eukaryotic cells, such as S. cerevisiae [101,102] mammalian cancer cells [103] with ferricyanide as an electron mediator, suggesting that conducting polymers could be coated on general living cells to facilitate EET. The construction of such hybrid films of conductive polymers and microbial cells is a promising approach for increasing MFC output.…”

Section: Conductive Polymers Capable Of Enhancing the Eetmentioning

confidence: 99%

Redox‐Active Polymers Connecting Living Microbial Cells to an Extracellular Electrical Circuit

Kaneko

Ishihara

Nakanishi

2020

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Microbial electrochemical systems in which metabolic electrons in living microbes have been extracted to or injected from an extracellular electrical circuit have attracted considerable attention as environmentally‐friendly energy conversion systems. Since general microbes cannot exchange electrons with extracellular solids, electron mediators are needed to connect living cells to an extracellular electrode. Although hydrophobic small molecules that can penetrate cell membranes are commonly used as electron mediators, they cannot be dissolved at high concentrations in aqueous media. The use of hydrophobic mediators in combination with small hydrophilic redox molecules can substantially increase the efficiency of the extracellular electron transfer process, but this method has side effects, in some cases, such as cytotoxicity and environmental pollution. In this Review, recently‐developed redox‐active polymers are highlighted as a new type of electron mediator that has less cytotoxicity than many conventional electron mediators. Owing to the design flexibility of polymer structures, important parameters that affect electron transport properties, such as redox potential, the balance of hydrophobicity and hydrophilicity, and electron conductivity, can be systematically regulated.

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Section: Conductive Polymers Capable Of Enhancing the Eetmentioning

confidence: 99%

Redox‐Active Polymers Connecting Living Microbial Cells to an Extracellular Electrical Circuit

Kaneko

Ishihara

Nakanishi

2020

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“…We demonstrated that these kinds of Ppy formation redox processes, which are involved in metabolism occurring in living cells, can be adapted even without any redox mediators [46]. Our studies based on the nonradioactive isotope method illustrated that Ppy formed during microbial polymerization is deposited mainly within the cell wall and in the space between the cell wall and cell membrane [47].…”

Section: Figurementioning

confidence: 80%

Conducting Polymers in the Design of Biosensors and Biofuel Cells

2020

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Fast and sensitive determination of biologically active compounds is very important in biomedical diagnostics, the food and beverage industry, and environmental analysis. In this review, the most promising directions in analytical application of conducting polymers (CPs) are outlined. Up to now polyaniline, polypyrrole, polythiophene, and poly(3,4-ethylenedioxythiophene) are the most frequently used CPs in the design of sensors and biosensors; therefore, in this review, main attention is paid to these conducting polymers. The most popular polymerization methods applied for the formation of conducting polymer layers are discussed. The applicability of polypyrrole-based functional layers in the design of electrochemical biosensors and biofuel cells is highlighted. Some signal transduction mechanisms in CP-based sensors and biosensors are discussed. Biocompatibility-related aspects of some conducting polymers are overviewed and some insights into the application of CP-based coatings for the design of implantable sensors and biofuel cells are addressed. New trends and perspectives in the development of sensors based on CPs and their composites with other materials are discussed.

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“…In situ formation of synthetic polymers catalysed by live cellular activity has been demonstrated for the self‐labelling of bacteria and enhancing functional and chemical properties in yeast and bacteria . In addition, Saccharomyces cerevisiae has been reported to be able to mediate polymerisation of pyrrole. The analogous mammalian‐cell‐instructed synthesis could have potential applications ranging from enhanced early‐stage cancer cell detection, through targeting of abnormal cells by means of in situ cytotoxic polymer production, to polymer “cell‐painting” in order to induce immune system attack.…”

Section: Figurementioning

confidence: 99%

Mammalian‐Cell‐Driven Polymerisation of Pyrrole

et al. 2019

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A model cancer cell line was used to initiate polymerisation of pyrrole to form the conducting material polypyrrole. The polymerisation was shown to occur through the action of cytosolic exudates rather than that of the membrane redox sites that normally control the oxidation state of iron as ferricyanide or ferrocyanide. The data demonstrate for the first time that mammalian cells can be used to initiate synthesis of conducting polymers and suggest a possible route to detection of cell damage and/or transcellular processes through in situ and amplifiable signal generation.

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Yeast-assisted synthesis of polypyrrole: Quantification and influence on the mechanical properties of the cell wall

Cited by 35 publications

References 53 publications

Redox‐Active Polymers Connecting Living Microbial Cells to an Extracellular Electrical Circuit

Redox‐Active Polymers Connecting Living Microbial Cells to an Extracellular Electrical Circuit

Conducting Polymers in the Design of Biosensors and Biofuel Cells

Mammalian‐Cell‐Driven Polymerisation of Pyrrole

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