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Карлов, Г. И.; Danilova, Tatiana; Horlemann, C.; Weber, G.

doi:10.1023/a:1024646818324

Cited by 36 publications

(5 citation statements)

References 26 publications

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“…With regard to the chromosomal composition of hop, classical cytogenetics has established that the species has heteromorphic sex chromosomes and occasional meiotic quadrivalents of unknown chromosomal composition (Sinotô, 1929;Neve, 1958;Haunold, 1991;Shephard and Parker, 2000). More recently, somatic hop karyotypes have been developed for several varieties, including FISH mapping of the locations of the NOR, 5S rDNA and the abundant Humulus subtelomeric repeats, HSR1 (Karlov et al, 2003;Divashuk et al, 2011). Functional genomics in hop has been advanced by detailed linkage analysis (Henning et al, 2017) and whole-genome sequencing (Natsume et al, 2015), yet these data are not integrated into a single annotated reference genome nor connected to the chromosome numbers of the published karyotypes.…”

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confidence: 99%

Non‐Mendelian Single‐Nucleotide Polymorphism Inheritance and Atypical Meiotic Configurations are Prevalent in Hop

Zhao

Easterling²,

Pitra³

et al. 2017

The Plant Genome

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Hop (Humulus lupulus L.) breeding programs seek to exploit genetic resources for bitter flavor, aroma, and disease resistance. However, these efforts have been thwarted by segregation distortion including female-biased sex ratios. To better understand the transmission genetics of hop, we genotyped 4512 worldwide accessions of hop, including cultivars, landraces, and over 100 wild accessions using a genotyping-by-sequencing (GBS) approach. From the resulting ~1.2 million single-nucleotide polymorphisms (SNPs), prequalified GBS markers were validated by inferences in population structures and phylogeny. Analysis of pseudo-testcross (Pt) mapping data from F 1 families revealed mixed patterns of Mendelian and non-Mendelian segregation. Three-dimensional (3D) cytogenetic analysis of late meiotic prophase nuclei from two wild and two cultivated hop revealed conspicuous and prevalent occurrences of multiple, atypical, nondisomic chromosome complexes including autosomes. We used genome-wide association studies (GWAS) and fixation index (F st ) analysis to demonstrate selection mapping of genetic loci for key traits including sex, bitter acids, and drought tolerance. Among the possible mechanisms underlying the observed segregation distortion from the genomic data analysis, the cytogenetic analysis points to meiotic chromosome behavior as one of the contributing factors. The findings shed light on long-standing questions on the unusual transmission genetics and phenotypic variation in hop, with major implications for breeding, cultivation, and the natural history of Humulus.

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confidence: 99%

Non‐Mendelian Single‐Nucleotide Polymorphism Inheritance and Atypical Meiotic Configurations are Prevalent in Hop

Zhao

Easterling²,

Pitra³

et al. 2017

The Plant Genome

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“…The sex determination system is similar in Humulus, Cannabis and Rumex species, where the XX/XY system exists and the ratio between X chromosomes and autosomes effects sex expression 8,19 . In the family Cannabaceae, the three species – Cannabis sativa, Humulus lupulus and Humulus japonicus – are dioecious with heterogametic sex chromosomes.…”

Section: Discussionmentioning

confidence: 99%

“…lupulus ) is normally diploid (2n = 2x = 20) with nine autosomal bivalents and two heteromorphic sex chromosomes. Female plants have a pair of X chromosomes, while male plants have an XY pair with the Y chromosome being smaller 8,17–19 . In all other species of the genus Humulus , such as H. lupulus var.…”

Section: Introductionmentioning

confidence: 99%

New Male Specific Markers for Hop and Application in Breeding Program

Čerenak

Kolenc

Sehur

et al. 2019

Sci Rep

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Male specific DNA sequences were selected from a Diversity Arrays Technology (DArT) mapping study to evaluate their suitability for determination of the sex phenotype among young seedlings in a hop (Humulus lupulus L.) breeding program. Ten male specific DArT markers showed complete linkage with male sex phenotype in three crossing families. Following optimization, four were successfully converted into PCR markers and a multiplex PCR approach for their use was developed. Among 197 plants (97 from the world collection; 100 from three segregating families), 94–100% positive correlation with sex phenotypic data was achieved for the single PCR amplification, whereas the multiplex approach showed 100% correlation. To develop a fast and low-cost method, crude sample multiplex PCR was evaluated in 253 progenies from 14 segregating populations without losing accuracy. The study describes, for the first time, the routine application of molecular markers linked to male sex in an intensive Slovenian hop breeding program. The methods described could be employed for screening of sex at the seedling stage in other hop programs worldwide, thereby saving resources for desirable female plants.

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“…CS-1 (a clone preserved from the research by Divashuk et al, 2014), 5S rDNA, and 18S-28S rDNA were labeled by nick-translation with digoxigenin-11-dUTP, and CS-237 was labeled by PCR with biotin-16-dUTP according to the manufacturer’s instructions (Boehringer, Ingelheim am Rhein, Germany). The FISH experiments were performed as described by Karlov et al [ 70 ]. The stringency of the FISH was about 72% (washing conditions: 15 min in 0.1× SSC at 42 °C).…”

Section: Methodsmentioning

confidence: 99%

Study and Physical Mapping of the Species-Specific Tandem Repeat CS-237 Linked with 45S Ribosomal DNA Intergenic Spacer in Cannabis sativa L.

Alexandrov

Romanov

Divashuk

et al. 2022

Plants

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Hemp (Cannabis sativa L.) is a valuable crop and model plant for studying sex chromosomes. The scientific interest in the plant has led to its whole genome sequencing and the determination of its cytogenetic characteristics. A range of cytogenetic markers (subtelomeric repeat CS-1, 5S rDNA, and 45S rDNA) has been mapped onto hemp’s chromosomes by fluorescent in situ hybridization (FISH). In this study, another cytogenetic marker (the tandem repeat CS-237, with a 237 bp monomer) was found, studied, and localized on chromosomes by FISH. The signal distribution and karyotyping revealed that the CS-237 probe was localized in chromosome 6 with one hybridization site and in chromosome 8 with two hybridization sites, one of which colocalizes with the 45S rDNA probe (with which a nucleolus organizer region, NOR, was detected). A BLAST analysis of the genomic data and PCR experiments showed that the modified CS-237 monomers (delCS-237, 208 bp in size) were present in the intergenic spacers (IGSs) of hemp 45S rDNA monomers. Such a feature was firstly observed in Cannabaceae species. However, IGS-linked DNA repeats were found in several plant species of other families (Fabaceae, Solanaceae, and Asteraceae). This phenomenon is discussed in this article. The example of CS-237 may be useful for further studying the phenomenon as well as for the physical mapping of hemp chromosomes.

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Untitled

Cited by 36 publications

References 26 publications

Non‐Mendelian Single‐Nucleotide Polymorphism Inheritance and Atypical Meiotic Configurations are Prevalent in Hop

Non‐Mendelian Single‐Nucleotide Polymorphism Inheritance and Atypical Meiotic Configurations are Prevalent in Hop

New Male Specific Markers for Hop and Application in Breeding Program

Study and Physical Mapping of the Species-Specific Tandem Repeat CS-237 Linked with 45S Ribosomal DNA Intergenic Spacer in Cannabis sativa L.

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