Wound-Induced Polyploidy Is Required for Tissue Repair

Losick, Vicki P.

doi:10.1089/wound.2014.0545

Cited by 27 publications

(27 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyploidy can confer cell-type and context specific benefits in various tissues. In Drosophila , polyploid intestinal enterocytes 76 , SPGs 19,20 and cells in the wounded epithelium 33,77 undergo endoreduplication and do not undergo cytokinesis to maintain the integrity of the blood brain barrier and the cell-cell junctions in the epithelium respectively. One possibility is that polyploidy in neurons or glia may allow cells to compensate for cell loss while maintaining established cell-cell contacts.…”

Section: What Is the Function Of Polyploidisation In The Brain?mentioning

confidence: 99%

Polyploidy in the adultDrosophilabrain

Nandakumar

Grushko

Buttitta

2019

Preprint

View full text Add to dashboard Cite

Long-lived cells such as terminally differentiated postmitotic neurons and glia must cope with the accumulation of damage over the course of an animal's lifespan. How long-lived cells deal with ageing-related damage is poorly understood. Here we show that polyploid cells accumulate in the ageing adult fly brain and that polyploidy protects against DNA damage-induced cell death. Multiple types of neurons and glia that are diploid at eclosion, become polyploid with age in the adult Drosophila brain. The optic lobes exhibit the highest levels of polyploidy, associated with an elevated DNA damage response in this brain region with age. Inducing oxidative stress or exogenous DNA damage leads to an earlier onset of polyploidy, and polyploid cells in the adult brain are more resistant to DNA damage-induced cell death than diploid cells. Our results suggest polyploidy may serve a protective role for neurons and glia in ageing Drosophila melanogaster brains. for providing fly stocks, particularly the labs of Cheng-

show abstract

Section: What Is the Function Of Polyploidisation In The Brain?mentioning

confidence: 99%

Polyploidy in the adultDrosophilabrain

Nandakumar

Grushko

Buttitta

2019

Preprint

View full text Add to dashboard Cite

show abstract

“…23,[48][49][50][51] In addition, a significant increase in DNA content has been reported for cells from different tissues under inflammatory or hypoxia conditions. 45,52,53 Inflammation is one of the main features of KCS and was observed in all KCS eyes of this study. Also, KCS leads to hypertonicity of tears with subsequent hypoxia of the ocular surface.…”

Section: F I G U R E 5 Surface Plot Graphsmentioning

confidence: 59%

“…20,30 In this work, larger nuclei with high DNA content (ie near-tetraploid nuclei) were observed in both study groups and are typical of cells synthesizing DNA. 20 DNA synthesis (rise in ploidy) is an event that precedes mitosis and the proliferation of cells involved, for example, in the homeostasis of self-renewing adult tissues, 20,45 such as the conjunctival epithelium. Compared with the control group, the KCS group showed a high proportion of epithelial cells synthesizing DNA (51.7%), which indicates changes in tissue metabolism.…”

Section: F I G U R E 5 Surface Plot Graphsmentioning

confidence: 99%

Nuclear parameters and chromatin remodeling in epithelial cells and lymphocytes from the palpebral conjunctiva of dogs with keratoconjunctivitis sicca

Santos

Aldrovani

Filezio

et al. 2018

Veterinary Ophthalmology

View full text Add to dashboard Cite

show abstract

“…Indeed, polyploid cells are present throughout human lifespan, with some experts espousing the concept that acquisition of polyploid DNA content is an adaptive process to avoid cell death as well as oncogenic transformation 46,47 . Regenerative activity and functional specialization of cells in mammals are associated with polyploid cell populations 48,49 . In addition, ploidy levels can be influenced as a consequence of aging, stress, acute injury, and environmental conditions 1,2,11,13 .…”

Section: Discussionmentioning

confidence: 99%

Cardiac interstitial tetraploid cells that escape replicative senescence are found in rodents but not large mammals

Broughton

Khieu

Nguyen

et al. 2019

Preprint

View full text Add to dashboard Cite

Ploidy for cardiomyocytes is well described but remains obscure in cardiac interstitial cells (CICs). Ploidy of c-kit+CICs (cCICs) were assessed using a combination of confocal, karyotypic, and flow cytometric assessments coupled with molecular and bioinformatic analyses. Fundamental differences were found between cultured rodent (rat, mouse) cCICs possessing mononuclear tetraploid (4n) content versus large mammal (human, swine) with mononuclear diploid (2n) content. In-situ analysis, confirmed with fresh isolates, revealed diploid content in cCICs from human and a mixture of diploid and tetraploid nuclei in mouse. Molecular assessment of the p53 signaling pathway provides a plausible explanation for escape from replicative senescence in rodent but not human cCICs. Single cell transcriptional profiling reveals distinctions between diploid versus tetraploid populations in mouse cCICs, alluding to functional divergences. Collectively, these data reveal fundamental species-specific biological differences in cCICs that could account for challenges in extrapolation of myocardial preclinical studies from rodent to large animal models.

show abstract

Wound-Induced Polyploidy Is Required for Tissue Repair

Cited by 27 publications

References 53 publications

Polyploidy in the adultDrosophilabrain

Polyploidy in the adultDrosophilabrain

Nuclear parameters and chromatin remodeling in epithelial cells and lymphocytes from the palpebral conjunctiva of dogs with keratoconjunctivitis sicca

Cardiac interstitial tetraploid cells that escape replicative senescence are found in rodents but not large mammals

Contact Info

Product

Resources

About