Wound healing improvement in large animals using an indirect helium plasma treatment

Martines, E.; Brun, Paola; Cavazzana, R.; Cordaro, Luigi; Zuin, M.; Martinello, Tiziana; Gomiero, Chiara; Perazzi, Anna; Melotti, Luca; Maccatrozzo, Lisa; Patruno, Marco; Iacopetti, Ilaria

doi:10.1016/j.cpme.2020.100095

Cited by 17 publications

(9 citation statements)

References 54 publications

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“…7). Most importantly, an array of in vivo data in noninfected wounds of different origins (e.g., punch biopsies, burn, and laser induced), animal species (e.g., mice, rats, and sheep), and locations (e.g., back and ear) has shown plasma treatment to promote healing in the absence of infection [113]- [120]. This markedly substantiates the idea of redox control of wound healing via plasma-derived ROS/RNS.…”

Section: A Current Statementioning

confidence: 99%

Low-Temperature Plasma for Biology, Hygiene, and Medicine: Perspective and Roadmap

Laroussi

Bekeschus

Keidar

et al. 2022

IEEE Trans. Radiat. Plasma Med. Sci.

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Plasma, the fourth and most pervasive state of matter in the visible universe, is a fascinating medium that is connected to the beginning of our universe itself. Man-made plasmas are at the core of many technological advances that include the fabrication of semiconductor devices, which enabled the modern computer and communication revolutions. The introduction of low temperature, atmospheric pressure plasmas to the biomedical field has ushered a new revolution in the healthcare arena that promises to introduce plasma-based therapies to combat some thorny and long-standing medical challenges. This article presents an overview of where research is at today and discusses innovative concepts and approaches to overcome present challenges and take the field to the next level. It is written by a team of experts who took an in-depth look at the various applications of plasma in hygiene, decontamination, and medicine, made critical analysis, and proposed ideas and concepts that should help the research community focus their efforts on clear and practical steps necessary to keep the field advancing for decades to come.

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Section: A Current Statementioning

confidence: 99%

Low-Temperature Plasma for Biology, Hygiene, and Medicine: Perspective and Roadmap

Laroussi

Bekeschus

Keidar

et al. 2022

IEEE Trans. Radiat. Plasma Med. Sci.

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“…Recently, Martines et al (2020) evaluated the use of a low-temperature atmospheric pressure plasma (ionized gas) as a treatment for extensive wounds in a sheep model. The plasma stimulated cell proliferation, angiogenesis and the development of skin adnexa; concomitantly, it reduced bacterial infection and inflammation ( Martines et al, 2020 ).…”

Section: Sheep As Animal Modelsmentioning

confidence: 99%

Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do

Ribitsch

Baptista

Consiglio

et al. 2020

Front. Bioeng. Biotechnol.

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126

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Rapid developments in Regenerative Medicine and Tissue Engineering has witnessed an increasing drive toward clinical translation of breakthrough technologies. However, the progression of promising preclinical data to achieve successful clinical market authorisation remains a bottleneck. One hurdle for progress to the clinic is the transition from small animal research to advanced preclinical studies in large animals to test safety and efficacy of products. Notwithstanding this, to draw meaningful and reliable conclusions from animal experiments it is critical that the species and disease model of choice is relevant to answer the research question as well as the clinical problem. Selecting the most appropriate animal model requires in-depth knowledge of specific species and breeds to ascertain the adequacy of the model and outcome measures that closely mirror the clinical situation. Traditional reductionist approaches in animal experiments, which often do not sufficiently reflect the studied disease, are still the norm and can result in a disconnect in outcomes observed between animal studies and clinical trials. To address these concerns a reconsideration in approach will be required. This should include a stepwise approach using in vitro and ex vivo experiments as well as in silico modeling to minimize the need for in vivo studies for screening and early development studies, followed by large animal models which more closely resemble human disease. Naturally occurring, or spontaneous diseases in large animals remain a largely untapped resource, and given the similarities in pathophysiology to humans they not only allow for studying new treatment strategies but also disease etiology and prevention. Naturally occurring disease models, particularly for longer lived large animal species, allow for studying disorders at an age when the disease is most prevalent. As these diseases are usually also a concern in the chosen veterinary species they would be beneficiaries of newly developed therapies. Improved awareness of the progress

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“…Several studies have been conducted in larger animal models to investigate the effects of gas plasma exposure on wound healing ( Table 3 ). In two studies using Bergamasca sheep, a novel helium plasma torch designed explicitly for veterinary purposes was studied [ 110 ]. The advantage of the source is its large treatment surface area, reducing the long exposure times required for larger wounds.…”

Section: Gas Plasma Treatment Of Skin and Wounds In Animal And Veterinary Modelsmentioning

confidence: 99%

“…The advantage of the source is its large treatment surface area, reducing the long exposure times required for larger wounds. In the first study, improved healing of experimental wounds was observed [ 110 ] along with a lower microbial burden. Subsequent wound tissue analysis revealed higher cell proliferation and VEGF presence in gas plasma-treated wounds than controls during early wound healing phases.…”

Section: Gas Plasma Treatment Of Skin and Wounds In Animal And Veterinary Modelsmentioning

confidence: 99%

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

2021

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The loss of skin integrity is inevitable in life. Wound healing is a necessary sequence of events to reconstitute the body’s integrity against potentially harmful environmental agents and restore homeostasis. Attempts to improve cutaneous wound healing are therefore as old as humanity itself. Furthermore, nowadays, targeting defective wound healing is of utmost importance in an aging society with underlying diseases such as diabetes and vascular insufficiencies being on the rise. Because chronic wounds’ etiology and specific traits differ, there is widespread polypragmasia in targeting non-healing conditions. Reactive oxygen and nitrogen species (ROS/RNS) are an overarching theme accompanying wound healing and its biological stages. ROS are signaling agents generated by phagocytes to inactivate pathogens. Although ROS/RNS’s central role in the biology of wound healing has long been appreciated, it was only until the recent decade that these agents were explicitly used to target defective wound healing using gas plasma technology. Gas plasma is a physical state of matter and is a partially ionized gas operated at body temperature which generates a plethora of ROS/RNS simultaneously in a spatiotemporally controlled manner. Animal models of wound healing have been vital in driving the development of these wound healing-promoting technologies, and this review summarizes the current knowledge and identifies open ends derived from in vivo wound models under gas plasma therapy. While gas plasma-assisted wound healing in humans has become well established in Europe, veterinary medicine is an emerging field with great potential to improve the lives of suffering animals.

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Wound healing improvement in large animals using an indirect helium plasma treatment

Cited by 17 publications

References 54 publications

Low-Temperature Plasma for Biology, Hygiene, and Medicine: Perspective and Roadmap

Low-Temperature Plasma for Biology, Hygiene, and Medicine: Perspective and Roadmap

Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

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