Background: Organizations are dependent on their employee for growth . So that emotion management in the work environment has become a significant problem due to its negative effect can take a heavy outcomes on employees as well as organization which cause stress and aggression. Aim: the study aimed to assess the relationship between emotional labor, workplace aggression and organizational role stress among nursing staff at Main Mansoura University Hospital. Method: A descriptive correlational design was used and the study was conducted on 169 nursing staff working at Main Mansoura University hospitals using three tools as: Emotional labor scale, workplace aggression scale and organizational role stress Scale. Results: the mean score of emotional labor was high (72.43±11.42) while the mean score of verbal aggression was higher than physical aggression with mean (1.33±0.38 and 1.27±0.35 respectively) also level of organizational role stress among nursing staff was low. There was statistically significant negative correlation between emotional labor and workplace aggression (p< 0.006) and organizational role stress (p< 0.0001). There was significant positive correlation between organizational role stress as well workplace aggression (p< 0.0001). Recommendation: Develop more flexible remuneration and reward systems to promote nursing staff satisfaction. Provide open channels of communication between top management and nursing staff and providing them with feedback on progress.