Women With and Without Metabolic Disorder Differ in Their Gut Microbiota Composition

Munukka, Eveliina; Wiklund, Petri; Pekkala, Satu; Völgyi, Eszter; Xu, Leiting; Cheng, Sihang; Lyytikäinen, Arja; Marjomäki, Varpu; Alén, Markku; Vaahtovuo, Jussi; Keinänen‐Kiukaanniemi, Sirkka; Cheng, Sulin

doi:10.1038/oby.2012.8

Cited by 88 publications

(63 citation statements)

References 29 publications

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“…As such, C leptum (cluster IV) has been associated with both obesity and weight loss (35,36) and was increased in OP and CVOP animals. Further, OTUs only present in OP and their GF recipients were assigned to Clostridium cluster XIVa, which is directly correlated with fat pad mass and BMI (35,37,38) and contains bacterial species known to break down polysaccharides, promoting monosaccharide absorption, enhanced lipogenesis, and lipid storage (4,20). In agreement with this, OP microbiota CV resulted in increase in adipogenic and lipogenic enzyme expression in the liver and adipose tissue as well as increased FA transporters, providing a more efficient transfer and storage of fermented byproducts.…”

Section: T H I S a R T I C L E H A S B E E N E T R A C T E D Rmentioning

confidence: 99%

Replication of Obesity and Associated Signaling Pathways Through Transfer of Microbiota From Obese-Prone Rats

et al. 2014

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Aberrations in gut microbiota are associated with metabolic disorders, including obesity. However, whether shifts in the microbiota profile during obesity are a characteristic of the phenotype or a consequence of obesogenic feeding remains elusive. Therefore, we aimed to determine differences in the gut microbiota of obese-prone (OP) and obeseresistant (OR) rats and examined the contribution of this microbiota to the behavioral and metabolic characteristics during obesity. We found that OP rats display a gut microbiota distinct from OR rats fed the same high-fat diet, with a higher Firmicutes-toBacteroidetes ratio and significant genera differences. Transfer of OP but not OR microbiota to germ-free (GF) mice replicated the characteristics of the OP phenotype, including reduced intestinal and hypothalamic satiation signaling, hyperphagia, increased weight gain and adiposity, and enhanced lipogenesis and adipogenesis. Furthermore, increased gut permeability through conventionalization resulted in inflammation by proinflammatory nuclear factor (NF)-kB/inhibitor of NF-kB kinase subunit signaling in adipose tissue, liver, and hypothalamus. OP donor and GF recipient animals harbored specific species from Oscillibacter and Clostridium clusters XIVa and IV that were completely absent from OR animals. In conclusion, susceptibility to obesity is characterized by an unfavorable microbiome predisposing the host to peripheral and central inflammation and promoting weight gain and adiposity during obesogenic feeding.

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Section: T H I S a R T I C L E H A S B E E N E T R A C T E D Rmentioning

confidence: 99%

Replication of Obesity and Associated Signaling Pathways Through Transfer of Microbiota From Obese-Prone Rats

et al. 2014

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“…Human studies have reported higher fecal concentrations of SCFA in overweight and obese humans compared with their normal-weight counterparts [7e9]. However, the changes in gut microbial composition at phylum and genus/species level that could explain differences between overweight and obese people remain matter of debate [2,7,10].…”

Section: Introductionmentioning

confidence: 98%

Inulin-type fructans modulate intestinal Bifidobacterium species populations and decrease fecal short-chain fatty acids in obese women

et al. 2015

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Section: Gomez-arango Et Al Blood Pressure and Gut Microbiome Butyratmentioning

confidence: 99%

“…A negative correlation between Bacteroides and blood pressure, body weight, and fat mass has been reported. 49,50 Therefore, supplementation with bacteria belonging to this genus may improve multiple clinical parameters.The gut microbiota can regulate ≈10% of the host's transcriptome, particularly genes involved in immune response, proliferation, and metabolism. 51 Preeclampsia incidence can be reduced by consumption of probiotics in dairy products.…”

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confidence: 99%

Increased Systolic and Diastolic Blood Pressure Is Associated With Altered Gut Microbiota Composition and Butyrate Production in Early Pregnancy

et al. 2016

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Abstract-The risk of developing pregnancy-induced hypertension and preeclampsia is higher in obese pregnant women.In obesity, the composition of the gut microbiota is altered. Obesity is also associated with low-grade inflammation.Metabolites from the gut microbiota may contribute to both hypertension and inflammation. The aim of this study is to investigate whether the composition of the gut microbiota in overweight and obese pregnant women is associated with blood pressure and levels of plasminogen activator inhibitor-1. The composition of the gut microbiota was determined with 16S ribosomal RNA sequencing in 205 women at 16 weeks gestation from the SPRING study (the Study of Probiotics in Gestational Diabetes). Expression of butyrate-producing genes in the gut microbiota was assessed by realtime polymerase chain reaction. Plasminogen activator inhibitor-1 levels were measured in fasting serum of a subset of 70 women. Blood pressure was slightly but significantly higher in obese compared with overweight women. The abundance of the butyrate-producing genus Odoribacter was inversely correlated with systolic blood pressure. Butyrate production capacity was decreased, but plasminogen activator inhibitor-1 concentrations increased in obese pregnant women. Plasminogen activator inhibitor-1 levels were inversely correlated with expression of butyrate kinase and Odoribacter abundance. This study shows that in overweight and obese pregnant women at 16 weeks gestation, the abundance of butyrate-producing bacteria and butyrate production in the gut microbiota is significantly negatively associated with blood pressure and with plasminogen activator inhibitor-1 levels. Increasing butyrate-producing capacity may contribute to maintenance of normal blood pressure in obese pregnant women. Gomez-Arango et al Blood Pressure and Gut Microbiome Butyrate Production 975The SCFA butyrate is produced from dietary fiber by bacteria in the gastrointestinal lumen. There are 2 main enzymes catalyzing butyrate production: butyrate kinase (Buk) and butyryl-CoA:acetate CoA-transferase (But), which are often used as biomarkers for the detection of butyrate-producing bacteria.11 Buk catalyzes the formation of butyrate from butyryl-CoA, releasing ATP in the process. 12,13 But catalyzes the reaction of butyryl-CoA with exogenous acetate to form butyrate and acetyl-CoA.13,14 Some butyrate-producing bacteria only express But or Buk, whereas others express both. 12The main butyrate producers in the human gut belong to the Firmicutes phylum (Coprococci, Eubacterium, Roseburia, and Faecalibacterium genera), 14 but members of other phyla, especially Bacteroidetes (Odoribacter and Alistipes genera), often contribute to the overall butyrogenic pool. SCFAproducing bacteria may affect blood pressure by direct effects of SCFA on vasodilation or through plasminogen activator inhibitor-1 (PAI-1). n-Butyrate increases PAI-1 mRNA in cultured hepatocytes, 15 and SCFA enemas stimulate rectal microcirculation and PAI-1 after aortic graft surgery. 16 It is uncl...

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Women With and Without Metabolic Disorder Differ in Their Gut Microbiota Composition

Cited by 88 publications

References 29 publications

Replication of Obesity and Associated Signaling Pathways Through Transfer of Microbiota From Obese-Prone Rats

Replication of Obesity and Associated Signaling Pathways Through Transfer of Microbiota From Obese-Prone Rats

Inulin-type fructans modulate intestinal Bifidobacterium species populations and decrease fecal short-chain fatty acids in obese women

Increased Systolic and Diastolic Blood Pressure Is Associated With Altered Gut Microbiota Composition and Butyrate Production in Early Pregnancy

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