WITHDRAWN: OxyR-Like Improve Cell's Hydrogen Peroxide Tolerance via Participate in Monocyte Chemotaxis and Oxidative Phosphorylation Regulation in Magnetospirillum Gryphiswaldense MSR-1

Abstract

Magnetosome formation inside magnetotactic bacteria is a complex process strictly regulated by intracellular metabolic regulation system. A series of transcriptional regulators have been proved involved in magnetosome bio-synthesis, including OxyR-Like, which is indispensable during magnetosome maturation in Magnetospirillum gryphiswaldense MSR-1. In this study, a new function of OxyR-Like was identified that it helps cell to defense reactive oxygen species (ROS). Comparison of expression profile data between wild type MSR-1 and oxyR-Like defective mutant demonstrate that after the disruption of oxyR-Like, 7 genes encoding chemotaxis proteins were down-regulated. On the contrary, the expression level of numerous genes encoding proteins which are critical for cellular aerobic respiration are increased. The result suggesting a model that OxyR-Like enhance the cell’s ROS resistant ability via increasing its environmental perception and keeping its oxidative phosphorylation in a reasonable level to avoid excessive production of endogenous ROS. This study further enriched the regulatory network of OxyR-Like and established the relationship between antioxidant metabolism pathway and magnetosome biomineralization in MSR-1.