Winter disruption of the circadian clock in chestnut

Ramos, Alberto; Pérez-Solís, Estefanía; Ibáñez, Cristian; Casado, Rosa; Collada, Carmen; Gómez, Luis; Aragoncillo, Cipriano; Allona, Isabel

doi:10.1073/pnas.0408549102

Cited by 143 publications

(158 citation statements)

References 52 publications

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“…the basic clock components of circadian systems are likely conserved among flowering plants. Recently, clock-related homologs with essentially conserved expression profiles have been isolated from several plant species (Boxall et al, 2005;Ramos et al, 2005;Murakami et al, 2007). Our studies using Lemna strongly support the idea that those clock-related homologs have conserved functions in the various circadian oscillators.…”

Section: Discussionsupporting

confidence: 64%

“…On the basis of sequence similarities to the Arabidopsis clock-related genes, homologous genes were isolated from a number of plants (Boxall et al, 2005;Ramos et al, 2005;Murakami et al, 2007). Comprehensive analysis was carried out in rice (Oryza sativa) after the complete genomic sequence of this model monocotyledonous plant was determined (Murakami et al, 2007).…”

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confidence: 99%

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Functional Conservation of Clock-Related Genes in Flowering Plants: Overexpression and RNA Interference Analyses of the Circadian Rhythm in the Monocotyledon Lemna gibba

et al. 2008

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Circadian rhythms are found in organisms from cyanobacteria to plants and animals. In flowering plants, the circadian clock is involved in the regulation of various physiological phenomena, including growth, leaf movement, stomata opening, and floral transitions. Molecular mechanisms underlying the circadian clock have been identified using Arabidopsis (Arabidopsis thaliana); the functions and genetic networks of a number of clock-related genes, including CIRCADIAN CLOCK ASSOCI-ATED1, LATE ELONGATED HYPOCOTYL (LHY), TIMING OF CAB EXPRESSION1, GIGANTEA (GI), and EARLY FLOWER-ING3 (ELF3), have been analyzed. The degree to which clock systems are conserved among flowering plants, however, is still unclear. We previously isolated homologs for Arabidopsis clock-related genes from monocotyledon Lemna plants. Here, we report the physiological roles of these Lemna gibba genes (LgLHYH1, LgLHYH2, LgGIH1, and LgELF3H1) in the circadian system. We studied the effects of overexpression and RNA interference (RNAi) of these genes on the rhythmic expression of morning-and evening-specific reporters. Overexpression of each gene disrupted the rhythmicity of either or both reporters, suggesting that these four homologs can be involved in the circadian system. RNAi of each of the genes except LgLHYH2 affected the bioluminescence rhythms of both reporters. These results indicated that these homologs are involved in the circadian system of Lemna plants and that the structure of the circadian clock is likely to be conserved between monocotyledons and dicotyledons. Interestingly, RNAi of LgGIH1 almost completely abolished the circadian rhythm; because this effect appeared to be much stronger than the phenotype observed in an Arabidopsis gi loss-of-function mutant, the precise role of each clock gene may have diverged in the clock systems of Lemna and Arabidopsis.

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Section: Discussionsupporting

confidence: 64%

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confidence: 99%

Functional Conservation of Clock-Related Genes in Flowering Plants: Overexpression and RNA Interference Analyses of the Circadian Rhythm in the Monocotyledon Lemna gibba

et al. 2008

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“…Although this microarray analysis is consistent with a loss of clock function at 48C, a longer time course will be required to confirm this finding. However, this apparent loss of rhythmicity has also been observed in chestnut (Castanea sativa), in which the levels of Cs LHY and Cs TOC1 dampened to a high level upon transfer to 48C, with LHY failing to repress TOC1 levels (Ramos et al, 2005).…”

Section: Temperature Regulation Of Clock-related Gene Expressionmentioning

confidence: 99%

The Molecular Basis of Temperature Compensation in the Arabidopsis Circadian Clock

et al. 2006

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Circadian clocks maintain robust and accurate timing over a broad range of physiological temperatures, a characteristic termed temperature compensation. In Arabidopsis thaliana, ambient temperature affects the rhythmic accumulation of transcripts encoding the clock components TIMING OF CAB EXPRESSION1 (TOC1), GIGANTEA (GI), and the partially redundant genes CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY). The amplitude and peak levels increase for TOC1 and GI RNA rhythms as the temperature increases (from 17 to 278C), whereas they decrease for LHY. However, as temperatures decrease (from 17 to 128C), CCA1 and LHY RNA rhythms increase in amplitude and peak expression level. At 278C, a dynamic balance between GI and LHY allows temperature compensation in wild-type plants, but circadian function is impaired in lhy and gi mutant plants. However, at 128C, CCA1 has more effect on the buffering mechanism than LHY, as the cca1 and gi mutations impair circadian rhythms more than lhy at the lower temperature. At 178C, GI is apparently dispensable for free-running circadian rhythms, although partial GI function can affect circadian period. Numerical simulations using the interlocking-loop model show that balancing LHY/CCA1 function against GI and other evening-expressed genes can largely account for temperature compensation in wild-type plants and the temperaturespecific phenotypes of gi mutants.

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“…Interestingly, GO analysis of the EBB1 putative target genes identified enrichment of a large number (n = 43) of biological processes (Dataset S4). Among the most represented/enriched were nitrogen metabolic processes (13), developmental process (11), response to stimulus (12), and regulation of transcription (6). Dormancy Induction and EBB1 Share Common Regulons.…”

Section: Ebb1 Transgenic Modifications Lead To Major Genome-wide Tranmentioning

confidence: 99%

“…For example, because light plays a major role in triggering growth cessation, photoreception via phytochromes has been viewed as an important control point in triggering the process (8,9). The integration of the light signal transduction into growth response is mediated via the FLOWERING TIME/ CONSTANS (FT/CO) module (10,11) and via regulatory proteins controlling circadian rhythms (12,13). In Arabidopsis, the ability of FT and other floral integrators to respond to inductive signals is controlled by a suite of MADS (MCM1, AGAMOUS, DEFICIENS, SRF) box genes like SHORT VEGETATIVE PHASE (SVP) and FLOWERING LOCUS C (FLC) (14).…”

mentioning

confidence: 99%

EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees

Yordanov

Strauss

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

126

116

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Trees from temperate latitudes transition between growth and dormancy to survive dehydration and freezing stress during winter months. We used activation tagging to isolate a dominant mutation affecting release from dormancy and identified the corresponding gene EARLY BUD-BREAK 1 (EBB1). We demonstrate through positioning of the tag, expression analysis, and retransformation experiments that EBB1 encodes a putative APETALA2/Ethylene responsive factor transcription factor. Transgenic up-regulation of the gene caused early bud-flush, whereas down-regulation delayed bud-break. Native EBB1 expression was highest in actively growing apices, undetectable during the dormancy period, but rapidly increased before bud-break. The EBB1 transcript was localized in the L1/L2 layers of the shoot meristem and leaf primordia. EBB1-overexpressing transgenic plants displayed enlarged shoot meristems, open and poorly differentiated buds, and a higher rate of cell division in the apex. Transcriptome analyses of the EBB1 transgenics identified 971 differentially expressed genes whose expression correlated with the EBB1 expression changes in the transgenic plants. Promoter analysis among the differentially expressed genes for the presence of a canonical EBB1-binding site identified 65 putative target genes, indicative of a broad regulatory context of EBB1 function. Our results suggest that EBB1 has a major and integrative role in reactivation of meristem activity after winter dormancy.adaptation | phenology | regeneration | climate change

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Winter disruption of the circadian clock in chestnut

Cited by 143 publications

References 52 publications

Functional Conservation of Clock-Related Genes in Flowering Plants: Overexpression and RNA Interference Analyses of the Circadian Rhythm in the Monocotyledon Lemna gibba

Functional Conservation of Clock-Related Genes in Flowering Plants: Overexpression and RNA Interference Analyses of the Circadian Rhythm in the Monocotyledon Lemna gibba

The Molecular Basis of Temperature Compensation in the Arabidopsis Circadian Clock

EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees

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