Why don’t biologists use SIMS?

Heeren, Ron M. A.; McDonnell, Liam A.; Amstalden, ER; Luxembourg, S.L.; Altelaar, Maarten; Piersma, S.R.

doi:10.1016/j.apsusc.2006.02.134

Cited by 101 publications

(81 citation statements)

References 54 publications

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“…It is becoming evident that diet influences membrane lipid composition associated with changes in the regulation of membranelinked cellular processes involved in energy metabolism, second messenger generation and gene expression. 71 ToF-SIMS membrane thus has a potential to help devise strategies for disease prevention.…”

Section: Research Questions In Biology and Medicine That Can Be Addrementioning

confidence: 99%

ToF‐SIMS imaging of lipids in cell membranes

Johansson¹

2006

Surface & Interface Analysis

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Section: Research Questions In Biology and Medicine That Can Be Addrementioning

confidence: 99%

ToF‐SIMS imaging of lipids in cell membranes

Johansson¹

2006

Surface & Interface Analysis

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“…21 No doubt this difference is the result of intense interest among SIMS researchers in better understanding the physical mechanisms underlying the technique. Likewise, SIMS is often believed to be inherently destructive and applicable only in the semiconductor field.…”

Section: Communicating the Utility Of Simsmentioning

confidence: 99%

Why Is SIMS Underused in Chemical and Biological Analysis? Challenges and Opportunities

Walker

2008

Anal. Chem.

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Improvements have led to many developments in SIMS, including better 2D MS imaging, the ability to perform molecular depth profiling, and the development of 3D MS imaging. (To listen to a podcast about this feature, please go to the Analytical Chemistry website at pubs.acs.org/ac.)Imaging MS has the unique ability to acquire the spatial distribution of a wide range of atoms and molecules, including polymers, pharmaceuticals, lipids, proteins, and semiconductors, without the use of labels such as fluorescent tags. Secondary ion MS (SIMS) imaging has advantages over MALDI imaging: it has submicrometer resolution and requires no sample preparation. Although MALDI imaging has been very successful, particularly in biological applications, 1 and has an increasing number of practitioners, SIMS imaging does not seem to be receiving the same amount of attention. In SIMS, ion beams are used to desorb molecules from surfaces; the mass of the molecules is then measured by MS. It was one of the first techniques developed for detecting organic molecules that were not amenable to electron impact ionization. 2,3It was largely overshadowed by other MS techniques, including ESI and MALDI, because in SIMS, it was difficult to desorb ions with m/z > 500, which makes the detection of large molecules very hard. In recent years, SIMS has undergone a renaissance with the advent of commercially available cluster ion sources: Au n + , Bi n x+ , SF 5 + , and C 60 x+ . [4][5][6][7][8][9][10][11] These are easy to use, reliable, and have long lifetimes (g500 µA/h). Cluster ion beams produce secondary ions with much higher efficiencies than atomic ion sources do, thereby increasing secondary ion yields and decreasing sample damage. These improvements have led to many developments in SIMS technology, including better 2D MS imaging, the ability to perform molecular depth profiling, and the development of 3D MS imaging. In this article, I will highlight some of the new applications enabled by cluster primary ion beams, particularly in biological imaging, and I will discuss the challenges of implementing SIMS more widely. (This article is not a comprehensive review of SIMS; for additional information, see Refs. 1-3 and 12-25.) IMAGE RESOLUTION AND CLUSTER ION SOURCESIn imaging MS, the image quality is determined by three different resolutions: mass, lateral, and depth. Mass resolution, m/∆m, is important because it determines the chemical specificity of the technique as well as its mass accuracy (through improved precision). For many years, commercially available TOF SIMS instruments have been able to produce m/∆m of several thousand in imaging mode.

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“…Very detailed images can be created, but to make sense of the meaning and relevance of novel peaks found in complex biological samples, the use of conventional tandem MS analysis of the sample will be required in order to complete the analysis. However, this will not necessarily produce the same results, due to sample processing or different ionization methods employed (Heeren et al 2006). …”

Section: Secondary Ion Mass Spectrometry (Sims)mentioning

confidence: 97%

“…Arguably one of the most intriguing and challenging scientific issues in recent times is the ability to investigate the distribution of biomolecules in specific organs and to spatiotemporally track their changes in response to abiotic and/or biotic stresses (Shrivas and Setou 2012) as well as production of attractants for pollinators and for communication with their surroundings (Bjarnholt et al 2014). This review concerns Mass Spectrometry Imaging (MSI), one of only a few techniques that could potentially replace and improve upon traditional fluorescence labelling or other staining techniques to improve the knowledge that biologists have of the spatial distribution of small molecules in plants (Heeren et al 2006). With the capabilities of MSI to track and map the spatial distribution of small molecules in plants, the data generated could be incorporated into metabolomic studies to analyse the geometrical changes in the plant metabolome in response to a certain stimulus from the environment.…”

Section: Introductionmentioning

confidence: 99%

The potential of mass spectrometry imaging in plant metabolomics: a review

Heyman

Dubery

2015

Phytochem Rev

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Imaging mass spectrometry (MSI) has contributed important information to several scientific fields and more recently to the biological sciences. The use of MSI as an investigative tool for plant metabolomics has recently been explored and initial results show a promising direction that plant metabolomics could be heading to with the use of these mass directed imaging techniques. Matrix assisted laser desorption ionisation (MALDI), secondary ion mass spectrometry and desorption electrospray ionisation mass spectrometry are the best-known MSI techniques used currently. MALDI-MSI is the most common used technique for molecular imaging. With new developments, spatial resolution capabilities have reached 5 lm which has resulted in a significant improvement in the data outputs for MSI analysis. Developments in the improvement of sample preparation techniques, instrumental improvements and more efficient and effective data analysis, have had a dramatic improvement in the obtained informational content. More recent developments on instrumentation and applications are seeing a change in direction towards the imaging of lower mass range metabolites. The unique feature that MSI brings to bio-analytical analysis is the possibility to analyse the distribution of hundreds of plant metabolites across a sample without any significant sample preparation. This has highlighted the potential of MSI techniques as ideal for metabolome analysis with the additional advantage of generating crucial spatial distribution information as well. The further development of MSI for the use in plant metabolomics is at an exciting time and the prospects for interesting new discoveries are very evident.

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Why don’t biologists use SIMS?

Cited by 101 publications

References 54 publications

ToF‐SIMS imaging of lipids in cell membranes

ToF‐SIMS imaging of lipids in cell membranes

Why Is SIMS Underused in Chemical and Biological Analysis? Challenges and Opportunities

The potential of mass spectrometry imaging in plant metabolomics: a review

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