Whole transcriptome analysis reveals differential gene expression profile reflecting macrophage polarization in response to influenza A H5N1 virus infection

Zhang, Na; Bao, Youmei; Tong, Amy Hin-Yan; Zuyderduyn, Scott; Bader, Gary D.; Peiris, J. S. Malik; Lok, Si; Lee, Smy

doi:10.1186/s12920-018-0335-0

Cited by 25 publications

(17 citation statements)

References 40 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA viruses, like influenza virus, can induce potent IFN antiviral responses which are strain and host cell specific(58). A highly relevant transcriptomic profiling of influenza infection in PBMC macrophages correlated an acute type I IFN response with an influenza virus strain with higher pathogenicity(59). Recent reports suggest that acute MNV infection is capable of IFN-response evasion(49), and particularly in the persistent murine norovirus strain MNV-S99(60).…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomic Response of Primary and Immortalized Macrophages to Murine Norovirus Infection

Levenson

Martens

Kanakabandi

et al. 2018

The Journal of Immunology

View full text Add to dashboard Cite

Murine norovirus (NoV) is genetically similar to human NoV and offers both an efficient in vitro cell culture system and an animal model by which to investigate the molecular basis of replication. In this study, we present a detailed global view of host alterations to cellular pathways that occur during the progression of a NoV infection. This was accomplished for both BALB/c-derived RAW264.7 (RAW) cells, an immortalized cell line widely used in in vitro replication studies, and primary bone marrow-derived macrophages (BMDM), representing a permissive in vivo target cell in the host. Murine NoV replicated in both cell types, although detected genome copies were approximately one log lower in BMDM compared with RAW cells. RAW and BMDM cells shared an IRF3/7-based IFN response that occurred early in infection. In RAW cells, transcriptional upregulation and INF-β expression were not coupled in that a significant delay in the detection of secreted INF-β was observed. In contrast, primary BMDM showed an early upregulation of transcripts and immediate release of INF-β that might account for lower virus yield. Differences in the transcriptional pathway responses included a marked decrease in expression of key genes in the cell cycle and lipid pathways in RAW cells compared with that of BMDM. Our comparative analysis indicates the existence of varying host responses to virus infection in populations of permissive cells. Awareness of these differences at the gene level will be important in the application of a given permissive culture system to the study of NoV immunity, pathogenesis, and drug development.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomic Response of Primary and Immortalized Macrophages to Murine Norovirus Infection

Levenson

Martens

Kanakabandi

et al. 2018

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Interestingly, H3N2 influenza virus subtype-infection induces comparable TNF-α, IL-12, IP-10, and IL-6 levels in both M1-like and M2-like monocytes and macrophages, and both macrophage types could be readily infected with this virus; an observation in marked contrast with the clearly distinct cytokine profiles of M1-like and M2-like macrophages exposed to bacterial products [ 63 ]. When analyzing the whole transcriptome of macrophages infected with highly pathogenic H5N1 influenza virus, another study showed that the activation state of the macrophage population is variable across time, suggesting that macrophage subtype switching is a highly plastic process [ 64 ].…”

Section: Swinging Macrophage Polarization During Infectionsmentioning

confidence: 99%

Role of Human Macrophage Polarization in Inflammation during Infectious Diseases

Atri

Guerfali

Laouini

2018

IJMS

985

811

View full text Add to dashboard Cite

Experimental models have often been at the origin of immunological paradigms such as the M1/M2 dichotomy following macrophage polarization. However, this clear dichotomy in animal models is not as obvious in humans, and the separating line between M1-like and M2-like macrophages is rather represented by a continuum, where boundaries are still unclear. Indeed, human infectious diseases, are characterized by either a back and forth or often a mixed profile between the pro-inflammatory microenvironment (dominated by interleukin (IL)-1β, IL-6, IL-12, IL-23 and Tumor Necrosis Factor (TNF)-α cytokines) and tissue injury driven by classically activated macrophages (M1-like) and wound healing driven by alternatively activated macrophages (M2-like) in an anti-inflammatory environment (dominated by IL-10, Transforming growth factor (TGF)-β, chemokine ligand (CCL)1, CCL2, CCL17, CCL18, and CCL22). This review brews the complexity of the situation during infectious diseases by stressing on this continuum between M1-like and M2-like extremes. We first discuss the basic biology of macrophage polarization, function, and role in the inflammatory process and its resolution. Secondly, we discuss the relevance of the macrophage polarization continuum during infectious and neglected diseases, and the possibility to interfere with such activation states as a promising therapeutic strategy in the treatment of such diseases.

show abstract

“…The transcriptome can aggregate a standard of analysis for the M2 phenotype characterized mainly by the expression of M130, CD163, CD206, MRC1, CCR7, МСР-3, FceRII, CD23, GPR86, GPR105, TLR-2, P2Y8, P2Y11, P2Y12, dectin-1, DC-SIGN, CD209, DCIR (CLECSF6), CLACSF13, FIZZ1, ST2 (SR-A, М60), CD184, TRAIL, STAT6, IL-27Ra, Chil3, Retnla, Ppar-γ, ARG1, TGF-β, IL-4, IL-5, IL-10, and IL-13 (Table 1). 85,86 It is noteworthy that transcriptome analysis has enabled elucidation of mechanisms underlying the modulation of IL-4 intracellular signaling by the induction of zinc finger TFs, Egr2, and c-Myc expression. As a final outcome, ingenuity pathway analysis shows that expression of these markers is directly involved in cell cycle progression in tumorigenesis.…”

Section: Markers Of M1 M2 and M4 Macrophagesmentioning

confidence: 99%

<p>Functional aspects, phenotypic heterogeneity, and tissue immune response of macrophages in infectious diseases</p>

Sousa¹,

Vasconcelos²,

Quaresma³

2019

IDR

View full text Add to dashboard Cite

Macrophages are a functionally heterogeneous group of cells with specialized functions depending not only on their subgroup but also on the function of the organ or tissue in which the cells are located. The concept of macrophage phenotypic heterogeneity has been investigated since the 1980s, and more recent studies have identified a diverse spectrum of phenotypic subpopulations. Several types of macrophages play a central role in the response to infectious agents and, along with other components of the immune system, determine the clinical outcome of major infectious diseases. Here, we review the functions of various macrophage phenotypic subpopulations, the concept of macrophage polarization, and the influence of these cells on the evolution of infections. In addition, we emphasize their role in the immune response in vivo and in situ, as well as the molecular effectors and signaling mechanisms used by these cells. Furthermore, we highlight the mechanisms of immune evasion triggered by infectious agents to counter the actions of macrophages and their consequences. Our aim here is to provide an overview of the role of macrophages in the pathogenesis of critical transmissible diseases and discuss how elucidation of this relationship could enhance our understanding of the host–pathogen association in organ-specific immune responses.

show abstract

Whole transcriptome analysis reveals differential gene expression profile reflecting macrophage polarization in response to influenza A H5N1 virus infection

Abstract: Our study provides important mechanistic insights into the understanding of H5N1 viral pathogenesis and the multi-faceted host immune responses. The dysregulated genes could be potential candidates as therapeutic targets for treating H5N1 disease.

Cited by 25 publications

References 40 publications

Comparative Transcriptomic Response of Primary and Immortalized Macrophages to Murine Norovirus Infection

Comparative Transcriptomic Response of Primary and Immortalized Macrophages to Murine Norovirus Infection

Role of Human Macrophage Polarization in Inflammation during Infectious Diseases

<p>Functional aspects, phenotypic heterogeneity, and tissue immune response of macrophages in infectious diseases</p>

Contact Info

Product

Resources

About