Whole-genome sequencing of degraded DNA for investigative genetic genealogy

Cady, Janet; Greytak, Ellen M.

doi:10.1016/j.fsigss.2022.09.008

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…As DNA sequencing becomes cheaper, whole genome sequencing (WGS) and massive parallel sequencing (MPS) have been developed for the generation of more accurate STR and SNP profile data for forensic use. In most cases using iFGG, SNP profile data have been generated using microarray genotyping [ 42 ]. However, this method requires more DNA than is usually present in crime scene samples.…”

Section: Dna In Criminal Investigationsmentioning

confidence: 99%

See 1 more Smart Citation

Law enforcement use of genetic genealogy databases in criminal investigations: Nomenclature, definition and scope

Tuazon,

Wickenheiser,

Ansell

et al. 2024

Forensic Science International: Synergy

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Section: Dna In Criminal Investigationsmentioning

confidence: 99%

“…Forensic samples are usually in the form of ‘touch DNA’, which contain low amounts of DNA [ 43 ]. In that regard, WGS has been reported to be a good alternative to produce SNP profile data for iFGG [ 42 ]. The DNA Doe Project makes use of WGS, from which SNP datasets are generated for upload to GEDmatch [ 40 ].…”

Section: Dna In Criminal Investigationsmentioning

confidence: 99%

Law enforcement use of genetic genealogy databases in criminal investigations: Nomenclature, definition and scope

Tuazon,

Wickenheiser,

Ansell

et al. 2024

Forensic Science International: Synergy

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“…Forensic genetic genealogy becomes more and more popular among law enforcement agencies in resolving cold cases and bringing back identity to unidentified human remains and has already proven its high success rate. For example, Oregon State Medical Examiner's Office together with Parabon NanoLabs managed to identify 30 out of 43 unidentified human remains using high-resolution SNP microarrays or whole genome sequencing and uploading the results to GEDmatch only (38).…”

Section: Practical Usementioning

confidence: 99%

The overview of forensic genetic genealogy

Rogalla-Ładniak

2023

AMSIK

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Forensic genetic genealogy (FGG) benefits largely from popularity of genealogical research within (mostly) American society and the advent of new sequencing techniques that allow typing of challenging forensic samples. It is considered a true breakthrough for both active and especially cold cases where all other resources and methods have failed during investigation. Despite media coverage generally highlighting its powers, the method itself is considered very laborious and the investigation may easily got suspended at every stage due to many factors including no hits in the database or breaks in traceable lineages within the family tree. This review summarizes the scope of FGG use, mentions most concerns and misconceptions associated with the technique and points to the plausible solutions already suggested. It also brings together current guidelines and regulations intended to be followed by law enforcement authorities wishing to utilize genetic genealogy research.

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Investigative genetic genealogy for human remains identification

Greytak,

Wyatt,

Cady

et al. 2024

Journal of Forensic Sciences

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Investigative genetic genealogy (IGG) has emerged as a highly effective tool for tying a forensic DNA sample to an identity. While much of the attention paid to IGG has focused on cases where the DNA is from an unknown suspect, IGG has also been used to help close hundreds of unidentified human remains (UHR) cases. Genome‐wide single‐nucleotide polymorphism (SNP) genotype data can be obtained from forensic samples using microarray genotyping or whole‐genome sequencing (WGS) with protocols optimized for degraded DNA. After bioinformatic processing, the SNP data can be uploaded to public GG databases that allow law enforcement usage, where it can be compared with other users' data to find distant relatives. A genetic genealogist can then build the family trees of the relatives to narrow down the identity of the source of the forensic DNA sample. To date, 36 UHR identifications using IGG have been publicly announced. The same IGG techniques developed and refined for UHR cases have significant potential for disaster victim identification, where DNA is often extremely compromised, and close family references may not be available. This paper reviews the laboratory, bioinformatic, and genealogical techniques used in IGG for UHR cases and presents three case studies that demonstrate how IGG is assisting with remains identification.

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Whole-genome sequencing of degraded DNA for investigative genetic genealogy

Cited by 3 publications

References 3 publications

Law enforcement use of genetic genealogy databases in criminal investigations: Nomenclature, definition and scope

Law enforcement use of genetic genealogy databases in criminal investigations: Nomenclature, definition and scope

The overview of forensic genetic genealogy

Investigative genetic genealogy for human remains identification

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