Whole-cell bacterial biosensors for the detection of aromatic hydrocarbons and their chlorinated derivatives (Review)

Плотникова, Е. Г.; Шумкова, Е. С.; Шумков, М. С.

doi:10.1134/s0003683816040128

Cited by 9 publications

(6 citation statements)

References 98 publications

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“…After genetic modification, the microorganism can produce reporter proteins under a promoter’s transcriptional control in a dose-dependent fashion in response to the presence of target compounds. 3 Thereby, whole-cell biosensors can provide a desirable signal output with very high sensitivity and selectivity. Whole-cell biosensors are a good alternative to enzyme- or antibody-based biosensors, since microbial cells can be grown rapidly at low cost, require little maintenance, and exhibit high selectivity.…”

Section: Introductionmentioning

confidence: 99%

“…Among them, living cells and especially genetically engineered microorganisms have shown advantages as biosensors. After genetic modification, the microorganism can produce reporter proteins under a promoter’s transcriptional control in a dose-dependent fashion in response to the presence of target compounds . Thereby, whole-cell biosensors can provide a desirable signal output with very high sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of Autoinducer Sensing Reporter Bacteria in Reinforced Alginate-Based Microbeads

Müller

Chang

et al. 2017

ACS Appl. Mater. Interfaces

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Quorum sensing, in which bacteria communities use signaling molecules for inter- and intracellular communication, has been intensively studied in recent decades. In order to fabricate highly sensitive easy-to-handle point of care biosensors that detect quorum sensing molecules, we have developed, as is reported here, reporter bacteria loaded alginate–methacrylate (alginate-MA) hydrogel beads. The alginate-MA beads, which were obtained by electrostatic extrusion, were reinforced by photo-cross-linking to increase stability and thereby to reduce bacteria leaching. In these beads the genetically engineered fluorescent reporter bacterium Escherichia coli pTetR-LasR-pLuxR-GFP (E. coli pLuxR-GFP) was encapsulated, which responds to the autoinducer N-(3-oxododecanoyl)homoserine lactone secreted by Pseudomonas aeruginosa. After encapsulation in alginate-MA hydrogel beads with diameters in the range of 100–300 μm that were produced by an electrostatic extrusion method and rapid photo-cross-linking, the E. coli pLuxR-GFP were found to possess a high degree of viability and sensing activity. The encapsulated bacteria could proliferate inside the hydrogel beads, when exposed to bacteria culture medium. In media containing the autoinducer N-(3-oxododecanoyl)homoserine lactone, the encapsulated reporter bacteria responded with a strong fluorescence signal due to an increased green fluorescent protein (GFP) expression. A prototype dipstick type sensor developed here underlines the potential of encapsulation of viable and functional reporter bacteria inside reinforced alginate–methacrylate hydrogel beads for whole cell sensors for bacteria detection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Encapsulation of Autoinducer Sensing Reporter Bacteria in Reinforced Alginate-Based Microbeads

Müller

Chang

et al. 2017

ACS Appl. Mater. Interfaces

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“…The deployment phase of a whole-cell microbial biosensor is challenging, particularly for devices that have environmental applications. The use of microbial biosensors in the environment and the associated challenges with their deployment have been extensively reviewed ( D’Souza, 2001 ; van der Meer et al, 2004 ; Harms et al, 2006 ; de las Heras and de Lorenzo, 2010 ; Coleman et al, 2011a ; de las Heras and de Lorenzo., 2012 ; Plotnikova et al, 2016 ; Shemer and Belkin, 2019 ; Hicks et al, 2020 ; Jiang et al, 2020 ); here we will focus on a few key engineering considerations relevant to the specific case of hydrocarbon biosensors. The potential of these systems has been long acknowledged, and yet the same barriers seem to stand in the way, decades after they were first recognized.…”

Section: Bringing Hydrocarbon Biosensors To Marketmentioning

confidence: 99%

“…There has been much research into the bacterial metabolism and detection of aromatic hydrocarbons ( Chauhan et al, 2008 ; Plotnikova et al, 2016 ; Reineke et al, 2020 ). Less is known about metabolism and sensing of aliphatic hydrocarbons, in particular the shorter chain gaseous compounds.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review

Moratti

Scott

Coleman

2022

Front. Bioeng. Biotechnol.

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Monooxygenases are a class of enzymes that facilitate the bacterial degradation of alkanes and alkenes. The regulatory components associated with monooxygenases are nature’s own hydrocarbon sensors, and once functionally characterised, these components can be used to create rapid, inexpensive and sensitive biosensors for use in applications such as bioremediation and metabolic engineering. Many bacterial monooxygenases have been identified, yet the regulation of only a few of these have been investigated in detail. A wealth of genetic and functional diversity of regulatory enzymes and promoter elements still remains unexplored and unexploited, both in published genome sequences and in yet-to-be-cultured bacteria. In this review we examine in detail the current state of research on monooxygenase gene regulation, and on the development of transcription-factor-based microbial biosensors for detection of alkanes and alkenes. A new framework for the systematic characterisation of the underlying genetic components and for further development of biosensors is presented, and we identify focus areas that should be targeted to enable progression of more biosensor candidates to commercialisation and deployment in industry and in the environment.

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“…Genetically modified organisms have also been coupled to optical fibers for the development of label-free biosensors for the sensitive and selective detection of a wide range of compounds of interest in different fields such as food analysis, environmental monitoring, and clinical diagnostics, among others [49,50,51,52,53]. Sensor performance depends on the introduction of a reporter gene into the host cell, whose expression is modulated by the interaction of the analyte with the molecular recognition element, and promoter sequences [54].…”

Section: Fiber Optic and Evanescent Wave Biosensorsmentioning

confidence: 99%

Optical Biosensors for Label-Free Detection of Small Molecules

Peltomaa

Glahn-Martínez

Benito‐Peña

et al. 2018

Sensors

165

111

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Label-free optical biosensors are an intriguing option for the analyses of many analytes, as they offer several advantages such as high sensitivity, direct and real-time measurement in addition to multiplexing capabilities. However, development of label-free optical biosensors for small molecules can be challenging as most of them are not naturally chromogenic or fluorescent, and in some cases, the sensor response is related to the size of the analyte. To overcome some of the limitations associated with the analysis of biologically, pharmacologically, or environmentally relevant compounds of low molecular weight, recent advances in the field have improved the detection of these analytes using outstanding methodology, instrumentation, recognition elements, or immobilization strategies. In this review, we aim to introduce some of the latest developments in the field of label-free optical biosensors with the focus on applications with novel innovations to overcome the challenges related to small molecule detection. Optical label-free methods with different transduction schemes, including evanescent wave and optical fiber sensors, surface plasmon resonance, surface-enhanced Raman spectroscopy, and interferometry, using various biorecognition elements, such as antibodies, aptamers, enzymes, and bioinspired molecularly imprinted polymers, are reviewed.

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Whole-cell bacterial biosensors for the detection of aromatic hydrocarbons and their chlorinated derivatives (Review)

Cited by 9 publications

References 98 publications

Encapsulation of Autoinducer Sensing Reporter Bacteria in Reinforced Alginate-Based Microbeads

Encapsulation of Autoinducer Sensing Reporter Bacteria in Reinforced Alginate-Based Microbeads

Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review

Optical Biosensors for Label-Free Detection of Small Molecules

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