Whole animal and gill tissue oxygen uptake in the Eastern oyster, Crassostrea virginica: Effects of hypoxia, hypercapnia, air exposure, and infection with the protozoan parasite Perkinsus marinus

Assessment of the Cell Viability of Cultured Perkinsus marinus (Perkinsea), a Parasitic Protozoan of the Eastern Oyster, Crassostrea virginica, Using SYBRgreen–Propidium Iodide Double Staining and Flow Cytometry

“…Oysters are ectothermic with body temperatures close to the ambient environment. For those living in the intertidal zone, a rapidly fluctuating environment could experience temperatures as high as 55 1C during low-tide exposure (Willson and Burnett 2000).…”

Assessment of the Cell Viability of Cultured Perkinsus marinus (Perkinsea), a Parasitic Protozoan of the Eastern Oyster, Crassostrea virginica, Using SYBRgreen–Propidium Iodide Double Staining and Flow Cytometry

“…Oysters are ectothermic with body temperatures close to the ambient environment. For those living in the intertidal zone, a rapidly fluctuating environment could experience temperatures as high as 55 1C during low-tide exposure (Willson and Burnett 2000).…”

EAT BREATHE EXCRETE REPEAT: Physiological Responses of the Mussel Mytilus galloprovincialis to Diclofenac and Ocean Acidification

“…In this study, OA significantly reduced RR at T0. Similar findings were reported in other studies on various bivalve species, such as M. galloprovincialis [50], Crassostrea virginica [51], Crassostrea gigas [52], Ruditapes decussatus [53], and C. nobilis [28]. In bivalves, oxygen consumption often increases under exposure to environmental contaminants [34].…”

Impact of Ocean Acidification on Energy Metabolism of Oyster, Crassostrea gigas—Changes in Metabolic Pathways and Thermal Response

“…In the pilot experiments, we measured cellular respiration using buffer B adjusted for pH and P co 2 to mimic the hemolymph parameters of control and CO 2 -exposed oysters, respectively. The elevated p CO 2 of the buffer had no effect on cell respiration (data not shown) which agrees with the results of previous studies [68,69]; therefore, in the subsequent experiments, only pH was adjusted to simplify the experimental logistics.…”

Assessment of the Cell Viability of Cultured Perkinsus marinus (Perkinsea), a Parasitic Protozoan of the Eastern Oyster, Crassostrea virginica, Using SYBRgreen–Propidium Iodide Double Staining and Flow Cytometry

“…Oysters are ectothermic with body temperatures close to the ambient environment. For those living in the intertidal zone, a rapidly fluctuating environment could experience temperatures as high as 55 1C during low-tide exposure (Willson and Burnett 2000).…”

EAT BREATHE EXCRETE REPEAT: Physiological Responses of the Mussel Mytilus galloprovincialis to Diclofenac and Ocean Acidification

“…In this study, OA significantly reduced RR at T0. Similar findings were reported in other studies on various bivalve species, such as M. galloprovincialis [50], Crassostrea virginica [51], Crassostrea gigas [52], Ruditapes decussatus [53], and C. nobilis [28]. In bivalves, oxygen consumption often increases under exposure to environmental contaminants [34].…”

Impact of Ocean Acidification on Energy Metabolism of Oyster, Crassostrea gigas—Changes in Metabolic Pathways and Thermal Response

“…In the pilot experiments, we measured cellular respiration using buffer B adjusted for pH and P co 2 to mimic the hemolymph parameters of control and CO 2 -exposed oysters, respectively. The elevated p CO 2 of the buffer had no effect on cell respiration (data not shown) which agrees with the results of previous studies [68,69]; therefore, in the subsequent experiments, only pH was adjusted to simplify the experimental logistics.…”

Assessment of the Cell Viability of Cultured Perkinsus marinus (Perkinsea), a Parasitic Protozoan of the Eastern Oyster, Crassostrea virginica, Using SYBRgreen–Propidium Iodide Double Staining and Flow Cytometry

“…Oysters are ectothermic with body temperatures close to the ambient environment. For those living in the intertidal zone, a rapidly fluctuating environment could experience temperatures as high as 55 1C during low-tide exposure (Willson and Burnett 2000).…”

EAT BREATHE EXCRETE REPEAT: Physiological Responses of the Mussel Mytilus galloprovincialis to Diclofenac and Ocean Acidification

“…In this study, OA significantly reduced RR at T0. Similar findings were reported in other studies on various bivalve species, such as M. galloprovincialis [50], Crassostrea virginica [51], Crassostrea gigas [52], Ruditapes decussatus [53], and C. nobilis [28]. In bivalves, oxygen consumption often increases under exposure to environmental contaminants [34].…”

Impact of Ocean Acidification on Energy Metabolism of Oyster, Crassostrea gigas—Changes in Metabolic Pathways and Thermal Response

“…In the pilot experiments, we measured cellular respiration using buffer B adjusted for pH and P co 2 to mimic the hemolymph parameters of control and CO 2 -exposed oysters, respectively. The elevated p CO 2 of the buffer had no effect on cell respiration (data not shown) which agrees with the results of previous studies [68,69]; therefore, in the subsequent experiments, only pH was adjusted to simplify the experimental logistics.…”