WHIRLY1 Occupancy Affects Histone Lysine Modification and WRKY53 Transcription in Arabidopsis Developmental Manner

Huang, Dongmei; Lan, Wei; Li, Danjing; Deng, Ban; Lin, Wenfang; Ren, Yujun; Miao, Ying

doi:10.3389/fpls.2018.01503

Cited by 23 publications

(34 citation statements)

References 83 publications

(120 reference statements)

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“…This suggests that the accessibility of the chromatin at the WRKY53 promoter region can be driven back and forth, most likely to adjust WRKY53 expression and senescence induction to environmental conditions. Moreover, the single strand-binding protein WHIRLY1 binds to the opened promoter of WRKY53 and represses the enrichment of H3K4me3, but enhances the enrichment of H3K9ac at this gene locus [69]. On top of that, the WRKY53 protein can also directly interact with chromatin modifiers like HISTONE DEACETYLASE9 (HDA9) mediating the recruitment of the POWERDRESS/HDA9 complex to W-box containing promoter regions of key negative senescence regulators.…”

Section: Chromatin Remodeling: Suv2 Powerdress/hda9 Hac1 Jmj16mentioning

confidence: 99%

Arabidopsis WRKY53, a Node of Multi-Layer Regulation in the Network of Senescence

Zentgraf

Doll

2019

Plants

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Leaf senescence is an integral part of plant development aiming at the remobilization of nutrients and minerals out of the senescing tissue into developing parts of the plant. Sequential as well as monocarpic senescence maximize the usage of nitrogen, mineral, and carbon resources for plant growth and the sake of the next generation. However, stress-induced premature senescence functions as an exit strategy to guarantee offspring under long-lasting unfavorable conditions. In order to coordinate this complex developmental program with all kinds of environmental input signals, complex regulatory cues have to be in place. Major changes in the transcriptome imply important roles for transcription factors. Among all transcription factor families in plants, the NAC and WRKY factors appear to play central roles in senescence regulation. In this review, we summarize the current knowledge on the role of WRKY factors with a special focus on WRKY53. In contrast to a holistic multi-omics view we want to exemplify the complexity of the network structure by summarizing the multilayer regulation of WRKY53 of Arabidopsis.

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Section: Chromatin Remodeling: Suv2 Powerdress/hda9 Hac1 Jmj16mentioning

confidence: 99%

Arabidopsis WRKY53, a Node of Multi-Layer Regulation in the Network of Senescence

Zentgraf

Doll

2019

Plants

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“…Alterations in H3K4me3 and H3K9ac globally [54] and specifically at the WRKY53 locus [23,27,55] had been observed during plant senescence. The loss of nuclear isoforms of WHY1 upon exogenous H 2 O 2 treatment prompted us to check whether specific or global histone modification occurred under this condition.…”

Section: H 2 O 2 Induces the Enrichment Of H3k9ac And Rnap II At Wrkymentioning

confidence: 99%

“…The involvement of WHY1 protein in modulating telomere length by binding to the AT-rich region of telomeres has also been suggested [26]. We recently found that Arabidopsis WHY1 accumulation in the nucleus altered the enrichment of di/trimethylation of histone H3 at lysine 4 (H3K4me2/3) and H3K9ac and the recruitment of RNA polymerase II (RNAP II) at the promoter's AT-rich region of WRKY53, repressing WRKY53 transcription [27].…”

Section: Introductionmentioning

confidence: 99%

H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis

Lin

Huang

Shi

et al. 2019

Cells

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Leaf senescence, either as a natural stage of development or as an induced process under stress conditions, incorporates multiple intricate signaling pathways. At the cellular level, retrograde signals have been considered as important players during the initiation and progression of senescence in both animals and plants. The plant-specific single-strand DNA-binding protein WHIRLY1 (WHY1), a repressor of leaf natural senescence, is dually located in both nucleus and plastids. Despite many years of studies, the myth about its dual location and the underlying functional implications remain elusive. Here, we provide further evidence in Arabidopsis showing that alteration in WHY1 allocation between the nucleus and chloroplast causes perturbation in H2O2 homeostasis, resulting in adverse plant senescence phenotypes. The knockout of WHY1 increased H2O2 content at 37 days post-germination, coincident with an early leaf senescence phenotype, which can be rescued by ectopic expression of the nuclear isoform (nWHY1), but not by the plastid isoform (pWHY1). Instead, accumulated pWHY1 greatly provoked H2O2 in cells. On the other hand, exogenous H2O2 treatment induced a substantial plastid accumulation of WHY1 proteins and at the same time reduced the nuclear isoforms. This H2O2-induced loss of nucleus WHY1 isoform was accompanied by enhanced enrichments of histone H3 lysine 9 acetylation (H3K9ac) and recruitment of RNA polymerase II (RNAP II) globally, and specifically at the promoter of the senescence-related transcription factor WRKY53, which in turn activated WRKY53 transcription and led to a senescence phenotype. Thus, the distribution of WHY1 organelle isoforms and the feedback of H2O2 intervene in a circularly integrated regulatory network during plant senescence in Arabidopsis.

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“…These in silico studies were supported by in vivo evidence with WHIRLY1 being the first protein to be identified in the nucleus and plastids of the same plant cell (Grabowski et al ., 2008); however, its molecular function appears to be compartment‐specific. In the nucleus, WHIRLY1 is associated with WRKY53 promoter and acts as a suppressor of leaf senescence (Miao et al ., 2013; Huang et al ., 2018). In chloroplasts, WHIRLY1 is present in nucleoid fractions where it might be involved in the maintenance of plastid DNA stability (Maréchal et al ., 2009; Krupinska et al ., 2014).…”

Section: Introductionmentioning

confidence: 99%

CIA2 and CIA2‐LIKE are required for optimal photosynthesis and stress responses in Arabidopsis thaliana

et al. 2020

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SUMMARY Chloroplast‐to‐nucleus retrograde signaling is essential for cell function, acclimation to fluctuating environmental conditions, plant growth and development. The vast majority of chloroplast proteins are nuclear‐encoded, and must be imported into the organelle after synthesis in the cytoplasm. This import is essential for the development of fully functional chloroplasts. On the other hand, functional chloroplasts act as sensors of environmental changes and can trigger acclimatory responses that influence nuclear gene expression. Signaling via mobile transcription factors (TFs) has been recently recognized as a way of communication between organelles and the nucleus. In this study, we performed a targeted reverse genetic screen to identify dual‐localized TFs involved in chloroplast retrograde signaling during stress responses. We found that CHLOROPLAST IMPORT APPARATUS 2 (CIA2) has a functional plastid transit peptide, and can be located both in chloroplasts and the nucleus. Further, we found that CIA2, along with its homolog CIA2‐like (CIL) are involved in the regulation of Arabidopsis responses to UV‐AB, high light and heat shock. Finally, our results suggest that both CIA2 and CIL are crucial for chloroplast translation. Our results contribute to a deeper understanding of signaling events in the chloroplast‐nucleus cross‐talk.

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WHIRLY1 Occupancy Affects Histone Lysine Modification and WRKY53 Transcription in Arabidopsis Developmental Manner

Cited by 23 publications

References 83 publications

Arabidopsis WRKY53, a Node of Multi-Layer Regulation in the Network of Senescence

Arabidopsis WRKY53, a Node of Multi-Layer Regulation in the Network of Senescence

H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis

CIA2 and CIA2‐LIKE are required for optimal photosynthesis and stress responses in Arabidopsis thaliana

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