Whey Lactose as a Raw Material for Microbial Production of Biodegradable Polyesters

Koller, Martin; Salerno, Anna; Muhr, Alexander; Reiterer, Angelika; Chiellini, Emo; Casella, Sergio; Horvat, Predrag; Braunegg, Gerhart

doi:10.5772/48737

Cited by 30 publications

(38 citation statements)

References 104 publications

(92 reference statements)

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“…Only for the European Union, estimations quantify the annual whey production with more than 40 Mt per year, corresponding to more than 2 Mt of lactose; thereof, a considerable share is currently disposed into marine environments, provoking considerable ecological threats by its high biochemical oxygen demand [16][17][18][19][20][21][22]. Hence, new (bio) products from whey are aspired, especially such as those produced by the action of either Gram-positive or Gram-negative microbial production strains [16,23].…”

Section: Hydrolysis Of Mol Lactosementioning

confidence: 99%

“…As a way out, a great deal of research activities nowadays is focused on the application of carbonaceous industrial surplus streams as feedstocks. Just to mention a few, current literature makes us familiar with PHA biosynthesis starting from crude glycerol [7,8], from hydrolyzed polysaccharides [9,10], carbon dioxide from industrial effluent gases [11,12], other C1 compounds [13], waste lipids [14,15], or, as item of interest in the article at hand, lactose from dairy surplus whey [16].…”

Section: Introductionmentioning

confidence: 99%

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Comparing Chemical and Enzymatic Hydrolysis of Whey Lactose to Generate Feedstocks for Haloarchaeal Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) Biosynthesis

Koller¹,

Puppi²,

Braunegg³

2016

Int J Pharm Sci Res

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Whey lactose was hydrolyzed via different biocatalytic and chemical methods in order to establish the optimum procedure to generate a carbon-rich substrate for haloarchaeal production of the biopolyester poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV). Biocatalytic hydrolysis was carried out using the commercially available bacterial β-galactosidase enzyme formulation Maxilact LG 2000 TM and solid fungal β-galactosidase from Aspergillus niger. Different enzyme concentrations, incubation times, temperatures and pH-ranges were investigated to assess the optimum hydrolysis conditions. As major outcome of the undertaken investigation, an addition of 0.25% (v/v) Maxilact LG 2000 TM to whey permeate at pH-value 6.5 and 38°C leads to almost complete (more than 90% w/w) lactose hydrolysis already after only 5 h of stirring, and performs beneficial in terms of hydrolysis kinetics compared to the solid enzyme formulation. As an inexpensive alternative, kinetics of hydrolysis of whey lactose was investigated using different amounts of HCl or H 2 SO 4 , respectively, at 90°C. By adjusting the pH-value to 0.7 or lower and stirring at 90°C for 5 h, a degree of hydrolysis of about 90% (w/w) was achieved. The hydrolysis matter was used as carbon sources for PHBHV bioproduction by the haloarchaeal species Haloferax mediterranei. Independent of the applied hydrolysis method, PHBHV biopolyesters of similar monomeric composition, molar mass and dispersity index Di were accumulated by the strain. The final decision of the most adequate method in future will depend upon the microbial production strain and production scale.

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Section: Hydrolysis Of Mol Lactosementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparing Chemical and Enzymatic Hydrolysis of Whey Lactose to Generate Feedstocks for Haloarchaeal Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) Biosynthesis

Koller¹,

Puppi²,

Braunegg³

2016

Int J Pharm Sci Res

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“…Monômeros com maior presença de um isômero ótico permitem gerar um PLA com maior cristalinidade que um PLA obtido a partir de misturas racêmi-cas de monômeros, o que torna o uso de enzimas mais atrativo para aplicações comerciais devido a sua estereosseletividade. 51 …”

Section: Policaprolactonas E Polilactatosunclassified

Applications of Enzymes in Synthesis and Modification of Polymers

Albuquerque¹,

Ribeiro²,

Rabelo³

et al. 2014

Química Nova

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Recebido em 07/06/2013; aceito em 10/12/2013; publicado na web em 21/03/2014 APPLICATIONS OF ENZYMES IN SYNTHESIS AND MODIFICATION OF POLYMERS. Enzymes are biological catalysts that offer great potential for use in the synthesis and modification of polymers, being more specific and greener than chemical catalysts. In this work, enzymes from the classes of hydrolases (lipase, cutinase and protease) and of oxidoreductases (horseradish peroxidase, manganese peroxidase and laccase) were identified as the main biocatalysts responsible for the synthesis of polymers. Biocatalysis can potentially be part of the life cycle of several polymers, including polyesters, polyurethanes, polycarbonates, polyamides, functionalized polysaccharides and polystyrene, allowing the synthesis of specialty macromolecules for fine applications and with higher added-value than commodity polymers.Keywords: enzyme polymerization; biocatalysis; lipase. INTRODUÇÃOProcessos de polimerização representam uma das principais transformações químicas industriais da atualidade, sendo, em sua maioria, conduzidos na presença de catalisadores químicos não renováveis e sob elevadas temperaturas, demandando, em alguns casos, a proteção e desproteção de grupos funcionais, de forma a evitar a ocorrência de reações indesejadas. 1 Há cerca de 20 anos, a catálise enzimática tem sido apontada como uma estratégia de grande potencial para a síntese de políme-ros, por ser ambientalmente amigável e em geral bastante seletiva, permitindo assim a obtenção de macromoléculas com propriedades diferenciadas e de maior valor agregado.2 As enzimas para a síntese e também modificação de polímeros estão nas classes das hidrolases (como lipases, proteases e esterases) e das oxidorredutases. 3A biocatálise pode ser empregada tanto para a síntese de polí-meros naturais como de polímeros sintéticos, e dentre suas maiores vantagens está a elevada seletividade (enantio-, quimio-, éstereo-e régio-seletividade), levando à obtenção de polímeros perfeitamente estruturados na maioria dos casos. Além disso, a catálise enzimática ocorre em geral de forma bastante eficiente sob condições mais brandas de temperatura e pressão, 4 demanda reduzida (ou nenhuma) quantidade de solventes orgânicos, 5 não proporciona a formação de produtos secundários e os produtos finais são de fácil separação e recuperação.6-8 Tais vantagens ficam claras especialmente quando são empregados biocatalisadores na forma imobilizada, a qual é uma técnica amplamente reportada pela literatura.9,10 A biocatálise foi reconhecida na década de 1990 como uma área nova na síntese de polímeros de precisão. 11Dessa forma, o objetivo desse artigo é apresentar um panorama das principais enzimas e condições de processo que podem ser utilizadas para a síntese e a modificação de polímeros de interesse industrial. O reconhecimento da importância do tema se traduz pela existência hoje de livros inteiramente dedicados à polimerização enzimática. 12-14 APLICAÇÕES DE ENZIMAS NA SÍNTESE DE POLÍMEROS PoliésteresOs poliésteres estão dentre ...

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“…At present, only a few PHAs, typically from the structurally less complex class of scl-PHAs, are industrially produced. Especially in the case of the more complex mcl-PHAs, the disadvantage of high production costs has prevented their wide use despite their promising properties 1 . Even though fermentation processes were developed employing different kinds of bacteria to improve mcl-PHAs productivity 2,3 , there is still considerable room for improvement, including the area of process control.…”

Section: Introductionmentioning

confidence: 99%

Strategies for Automated Control of the Bioproduction of Mcl-PHA Biopolymers

Hrnčiřík¹

2017

Chem.Biochem.Eng.Q.

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Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are polyesters synthesized by numerous bacteria as storage material. Despite being promising candidates for biodegradable materials of industrial interest and environmental value, their usage is still rather limited because of high production costs. One of the areas with considerable potential for further improvements is control of the production process. This paper deals with the experimental work related to the design of control strategies for mcl-PHA biopolymer production process (Pseudomonas putida KT2442 fed-batch cultivations). For this bioprocess, a set of five control strategies (two main and three auxiliary strategies) have been proposed, together with the proper sequence of their switching during the fedbatch part of the production process. The application of these strategies with octanoic acid as a sole carbon source resulted in intracellular PHA content (max. mass fraction 65 % of mcl-PHA in cell dry mass (g g -1) and PHA productivity (max. 0.89 g L -1 h -1 ) comparable to the best results reported in the literature for this type of strain and carbon substrate.

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Whey Lactose as a Raw Material for Microbial Production of Biodegradable Polyesters

Cited by 30 publications

References 104 publications

Comparing Chemical and Enzymatic Hydrolysis of Whey Lactose to Generate Feedstocks for Haloarchaeal Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) Biosynthesis

Comparing Chemical and Enzymatic Hydrolysis of Whey Lactose to Generate Feedstocks for Haloarchaeal Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) Biosynthesis

Applications of Enzymes in Synthesis and Modification of Polymers

Strategies for Automated Control of the Bioproduction of Mcl-PHA Biopolymers

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