Whey fermented by Enterococcus faecalis M157 exhibits antiinflammatory and antibiofilm activities against oral pathogenic bacteria

Abstract

The aim of the present study was to investigate the antiinflammatory and antibiofilm effects of whey fermented by Enterococcus faecalis M157 (M157-W) against oral pathogenic bacteria. The M157-W significantly inhibited IL-1β, IL-6, and nitric oxide induced by the lipopolysaccharide of Porphyromonas gingivalis in RAW 264.7 cells. The M157-W also inhibited the production of IL-1β and IL-8 in human periodontal ligament cells. Treatment with M157-W suppressed the phosphorylation of mitogen-activated protein kinases as well as the activation of nuclear factor-κB in RAW 264.7 cells stimulated by P. gingivalis lipopolysaccharide. Furthermore, M157-W dose-dependently inhibited Streptococcus mutans biofilm, whereas unfermented whey did not inhibit the biofilm. Treatment with M157-W significantly suppressed gtfB, gtfC, and gtfD gene expression in S. mutans compared with the control (0 μg/mL), indicating that M157-W inhibits S. mutans biofilm formation by reducing the synthesis of extracellular polymeric substances. Collectively, these results suggest that M157-W has antiinflammatory and antibiofilm activities against oral pathogenic bacteria.

Deciphering the mechanism of anti‐quorum sensing post‐biotic mediators against Streptococcus mutans

“…Further, the CLSM analysis also revealed that co-treatment of S. mutans MTCC 890 bacterial cells with PMs PS-74 resulted in a dramatic transference of staining patterns with a strong increase of dead bacteria. These results align with a previous study by Dahyun Song et al (2022) which showed that CLSM confirmed S. mutans biofilm was less dense because of the presence of M157-W. More recently, L. plantarum showed an inhibitory effect on the biofilm formation and the growth of S. mutans (Ślebioda et al, 2016).…”

The Plethora of Microbes with Anti-Inflammatory Activities

“…The anti-inflammatory properties of whey fermented by Enterococcus faecalis M157 against oral cavity pathogens were estimated [ 104 ]. Bacterial pathogens cause chronic inflammation leading to periodontitis in the oral cavity, Porphyromonas gingivalis being the main causative.…”

“…The M157 strain successfully inhibited IL-1β, IL-6, and induced NO of Porphyromonas gingivalis in RAW 264.7 cells. IL-6 and IL-8 in human periodontal ligament cells were also inhibited [ 104 ].…”

In vitro and in silico characterization of N-formylated two-peptide bacteriocin from Enterococcus faecalis CAUM157 with anti-listeria activity

“…Ent. faecalis CAUM157 was recently implemented for postbiotic development through whey bioconversion to alleviate periodontitis in mouse models and investigate its anti-in ammatory and antibio lm effects against oral pathogens [30,31]. These studies demonstrated the protective properties and antimicrobial activities of M157 bacteriocins which provides evidence of their value and potential as a postbiotic product.…”