Whey Fermentation for Protease Production Using
&lt;i&gt;Bacillus thuringiensis&lt;/i&gt; Isolated from Mangrove
Rhizosphere Soil in Jazan, Saudi Arabia

El-Gayar, Khaled E.; Essa, Ashraf M. M.; Abada, Emad

doi:10.15244/pjoes/110583

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…In another study, whey was used as a substrate for the production of protease by a B. thuringiensis strain. The minimum medium supplemented with whey and skim milk presented 6000 mg L −1 dry weight, in which 2600 mg L −1 total proteins were recorded within 72 h of fermentation [19].…”

Section: Discussionmentioning

confidence: 99%

Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain

dos Santos,

de S. Varize,

Valença

et al. 2024

Beni-Suef Univ J Basic Appl Sci

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Background Bacillus thuringiensis (Bt) is a widely used biopesticide. The bioinsecticide based on Bt is obtained by fermentation, but the substrates currently used for its production constitute ingredients of high commercial value. In this context, the use of agro-industrial residues as substrates is an alternative to make the fermentation process viable on a large scale, in addition to minimizing environmental problems and contributing to the destination of these residues for biotechnological purposes. Results In the first part of this study, a previously isolated spore forming soil bacteria (Bv5) harboring and expressing a novel cry 8A gene was confirmed as B. thuringiensis based on its morphological characteristics, Gram staining, scanning electron microscopy (SEM) and genome sequencing. Bv5 was established as a Gram-positive spore forming bacteria with ellipsoidal spores and small round toxins. Bv5 genome comprised of the 5.30 Mb chromosome and two megaplasmids of 450 kb and 261 kb, respectively, with cry 8A gene located on the smallest megaplasmid. In the second part of the study, the physiological profile of the Bv5 strain during fermentation in different agro-industrial biowastes (cassava wastewater, orange pulp wash and whey) was analyzed. The fermentation experiment was divided into two stages. In the first stage, the agro-industrial waste with or without salts with the best results for biomass, spores and proteins production was selected. In the second stage, the effect of the selected medium in original and diluted form with the C:N balance was evaluated, in two different fermentation times (72 h and 96 h). Pulp wash enriched with salts was selected as the most suitable medium for the growth of Bv5 strain in the first stage. In the second stage pulp wash (without dilution) with the addition of salts, and with nitrogen supplementation, was considered the best for cell growth, spore and toxin production by Bv5. Conclusions To conclude, our study provide a new alternative for bio-waste from the orange juice industry, as well as potential culture medium for the Bt commercial scale production.

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Section: Discussionmentioning

confidence: 99%

Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain

dos Santos,

de S. Varize,

Valença

et al. 2024

Beni-Suef Univ J Basic Appl Sci

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“…Milk is comprised of nutritive compounds such as caseins and whey proteins. Caseins constitute 80% of the total protein present in milk [32]. Therefore, microorganisms are considered sources of cellular growth and protease production.…”

Section: Discussionmentioning

confidence: 99%

Alkaline Protease Production from Bacillus gibsonii 6BS15-4 Using Dairy Effluent and Its Characterization as a Laundry Detergent Additive

Mahakhan

PatapeeAphaiso

Srisunthorn

et al. 2022

J. Microbiol. Biotechnol.

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Protease is a widely used enzyme particularly in the detergent industry. In this research, we aimed to isolate alkaline protease-producing bacteria for characterization as a laundry detergent additive. The screening of alkaline protease production was investigated on basal medium agar plus 1% skim milk at pH 11, with incubation at 30°C. The highest alkaline protease-producing bacterium was 6BS15-4 strain, identified as Bacillus gibsonii by 16S rRNA gene sequencing. While the optimum pH was 12.0, the strain was stable at pH range 7.0-12.0 when incubated at 45°C for 60 min. The alkaline protease produced by B. gibsonii 6BS15-4 using dairy effluent was characterized. The optimum temperature was 60°C and the enzyme was stable at 55°C when incubated at pH 11.0 for 60 min. Metal ions K + , Mg 2+ , Cu 2+ , Na + , and Zn 2+ exhibited a slightly stimulatory effect on enzyme activity. The enzyme retained over 80% of its activity in the presence of Ca 2+ , Ba 2+ , and Mn 2+ . Thiol reagent and ethylenediaminetetraacetic acid did not inhibit the enzyme activity, whereas phenylmethylsulfonyl fluoride significantly inhibited the protease activity. The alkaline protease from B. gibsonii 6BS15-4 demonstrated efficiency in blood stain removal and could therefore be used as a detergent additive, with potential for various other industrial applications.

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Immobilization of alkaline protease produced by Streptomyces rochei strain NAM-19 in solid state fermentation based on medium optimization using central composite design

El-Shazly,

Wahba,

Abdelwahed

et al. 2024

3 Biotech

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Whey Fermentation for Protease Production Using <i>Bacillus thuringiensis</i> Isolated from Mangrove Rhizosphere Soil in Jazan, Saudi Arabia

Cited by 7 publications

References 23 publications

Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain

Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain

Alkaline Protease Production from Bacillus gibsonii 6BS15-4 Using Dairy Effluent and Its Characterization as a Laundry Detergent Additive

Immobilization of alkaline protease produced by Streptomyces rochei strain NAM-19 in solid state fermentation based on medium optimization using central composite design

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