Wheat and gluten in South African food products

Cawthorn, Donna‐Mareè; Steinman, Harris; Witthuhn, R. Corli

doi:10.1080/09540100903443725

Cited by 9 publications

(3 citation statements)

References 31 publications

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“…The use of Proteinase K less than 30μl did not feasible for production of high quantity and purity of DNA while in present study we just used 7-20μl of Proteinase K which produced high quantity and purified DNA [26]. Isolated DNA with range of 1.7-2.0 at absorbance A 260 /A 280 is considered pure [27]. Present study showed that the GeneJET Genomic DNA Purification Kit has short incubation time and economic, as compared to analysed six traditional methods.…”

Section: Resultsmentioning

confidence: 99%

Comparison of seven genomic DNA isolation techniques from internal organs, gills and muscle tissues of Notopterus notopterus (Notopteridae) using PCR amplification and Nanodrop

Ahmad

2023

ijbch

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The techniques and the principles used in DNA isolation play a vital role in the obtaining of a purified genetic material. In present study, we investigated the efficiency of seven genomic DNA isolation techniques in terms of isolated DNA concentration, yield and purity from internal organs, gills and muscle tissues of Notopterus notopterus. Isolated DNA quality was analysed through Nanodrod and PCR using mitochondrial COI genetic markers. Results showed that GeneJET Genomic DNA Purification Kit was found significantly higher in terms of isolated DNA concentration (1200-1288 ng.ul -1 ), yield (257.6 ng.ul -1 ) and purity (1.91-2.00), and also successful in PCR amplification as compared to other evaluated six traditional DNA isolation methods. The nucleotide minimum bands range was observed 200 base pairs in heart sample and maximum 600 base pair was observed in intestine. There is no data on description of parameters analysed in this work to date, neither the evaluation of isolated DNA using PCR amplification of mitochondrial COI gene for the species N. notopterus. Present study also revealed that the traditional DNA isolation methods are the secondary choice for isolation of DNA. The data of present study also indicated that the GeneJET Genomic DNA Purification Kit is useful for DNA isolation and can be used best in genetic applications for fishes.

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Section: Resultsmentioning

confidence: 99%

Comparison of seven genomic DNA isolation techniques from internal organs, gills and muscle tissues of Notopterus notopterus (Notopteridae) using PCR amplification and Nanodrop

Ahmad

2023

ijbch

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“…sea bass. Another example is the severely overexploited red stumpnose "Chrysoblephus gibbiceps" that was sold as the white stumpnose "Rhabdosargus globiceps" [18] . In addition, in Canada, the Japanese amberjack "Seriola quinqueradiata" was sold as the yellowfin tuna "Thunnus albacares", which is categorized as near-threatened species in the IUCN red list of species [19] .…”

Section: Discussionmentioning

confidence: 99%

Application of Dna Barcoding for Identification of Some Key Fish-Market Species in Sharjah City, Uae

Abdelbaset-Donya

Hamza

Mohammed-Geba

et al. 2020

Egyptian Journal of Zoology

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“…Some other studies have used R5 Skerritt (38)(39)(40) or G12 (41) . Western blot has generally been used as a complementary method to ELISA (42,43) , and in parallel, some of the studies have used a PCR method (43)(44)(45)(46)(47)(48) (Tables 1, 2 and 3).…”

Section: Methods Used To Estimate the Prevalence Of Gluten Contaminationmentioning

confidence: 99%

Gluten contamination in labelled gluten-free, naturally gluten-free and meals in food services in low-, middle- and high-income countries: a systematic review and meta-analysis

et al. 2021

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The gluten-free diet is based on the consumption of foods without gluten, which aims to manage celiac disease. The concern of celiac patients is that these foods should be safe. However, gluten contamination can affect these foods. The objectives of this review and meta-analysis were first, to identify articles that detected gluten contamination in gluten-free foods using validated methods. Second, to quantify the overall prevalence of gluten contamination of naturally gluten-free foods, labelled gluten-free products, and meals prepared in food services. Third, to highlight the influence of the country’s income and the period of study on this prevalence. The studies were identified in Scopus, Science Direct, Web of Science, PubMed, and Google Scholar. Forty articles were included according to PRISMA guidelines. The statistical meta-analysis was performed using MedCalc 19 software. The results show that in the gluten-free foods analysed, the overall prevalence of gluten contamination was estimated at 15.12% (95% CI: 9.56%–21.70%), with more than 20 mg/kg of gluten. Naturally gluten-free foods were significantly more contaminated than labelled gluten-free products and than meals in food services (28.32%; 9.52%; 4.66% respectively; p < 0.001). Moreover, it was noticed that oats were the most contaminated food. In addition, the prevalence of gluten contamination has significantly decreased over time. The majority of the studies were carried out in upper-middle-income and high-income countries, while only one study was conducted in lower-middle income countries. Therefore, it is necessary to implement preventive actions to reduce gluten contamination, ensuring safe gluten-free foods for celiac patients, including low-income countries.

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Wheat and gluten in South African food products

Cited by 9 publications

References 31 publications

Comparison of seven genomic DNA isolation techniques from internal organs, gills and muscle tissues of Notopterus notopterus (Notopteridae) using PCR amplification and Nanodrop

Comparison of seven genomic DNA isolation techniques from internal organs, gills and muscle tissues of Notopterus notopterus (Notopteridae) using PCR amplification and Nanodrop

Application of Dna Barcoding for Identification of Some Key Fish-Market Species in Sharjah City, Uae

Gluten contamination in labelled gluten-free, naturally gluten-free and meals in food services in low-, middle- and high-income countries: a systematic review and meta-analysis

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