WFIKKN1 and WFIKKN2: “Companion” proteins regulating TGFB activity

Monestier, Olivier; Blanquet, Véronique

doi:10.1016/j.cytogfr.2016.06.003

Cited by 15 publications

(9 citation statements)

References 114 publications

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“…In our screen, one or more DCC subfamily members also bound to: (1) WFIKKN2, a secreted protein that binds transforming growth factor-beta subfamily members and modulates their presentation to cells ( Monestier and Blanquet, 2016 ), (2) lactotransferrin (LTF), an iron-binding protein with antimicrobial activity ( Hao et al., 2019 ), (3) interleukin-6 receptor subunit alpha (IL-6Rα), a cytokine receptor ( Schaper and Rose-John, 2015 ), and (4) ISLR2/LINX, which functions in nervous system development ( Mandai et al., 2014 ; Panza et al., 2015 ; Abudureyimu et al., 2018 ). We confirmed binding of all DCC subfamily members to these proteins by SPR and to other proteins observed in our screen ( Figures 3 C, 3D, and 3F).…”

Section: Resultsmentioning

confidence: 99%

A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions

Wojtowicz

Vielmetter

Fernandes

et al. 2020

Cell

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Section: Resultsmentioning

confidence: 99%

A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions

Wojtowicz

Vielmetter

Fernandes

et al. 2020

Cell

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“…1–4h) and that the 24h levels might be determined by the rate of removal for translation. In addition differences in TGFβ-mediated effects might result from a) modified turnover of TGFβ receptor proteins and/or associated kinase activities, b) altered levels of TGFβ “companion” proteins such as WFIKKN1 [23]) and/or c) over-production of TGFβ1 signaling antagonists such as decorin or IL-10 [24]).…”

Section: Resultsmentioning

confidence: 99%

Degenerative Suspensory Ligament Desmitis (DSLD) in Peruvian Paso Horses Is Characterized by Altered Expression of TGFβ Signaling Components in Adipose-Derived Stromal Fibroblasts

et al. 2016

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Equine degenerative suspensory ligament desmitis (DSLD) in Peruvian Paso horses typically presents at 7–15 years and is characterized by lameness, focal disorganization of collagen fibrils, and chondroid deposition in the body of the ligament. With the aim of developing a test for disease risk (that can be used to screen horses before breeding) we have quantified the expression of 76 TGFβ-signaling target genes in adipose-derived stromal fibroblasts (ADSCs) from six DSLD-affected and five unaffected Paso horses. Remarkably, 35 of the genes showed lower expression (p<0.05) in cells from DSLD-affected animals and this differential was largely eliminated by addition of exogenous TGFβ1. Moreover, TGFβ1-mediated effects on expression were prevented by the TGFβR1/2 inhibitor LY2109761, showing that the signaling was via a TGFβR1/2 complex. The genes affected by the pathology indicate that it is associated with a generalized metabolic disturbance, since some of those most markedly altered in DSLD cells (ATF3, MAPK14, ACVRL1 (ALK1), SMAD6, FOS, CREBBP, NFKBIA, and TGFBR2) represent master-regulators in a wide range of cellular metabolic responses.

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“…The gene VAV2 was also found to be down-regulated by the P1-AI treatment in the UTJ, which is in line with the development of an immune tolerance in the reservoir [48]. In addition, the P1-AI treatment caused an up-regulation of the WFIKKN2 gene, which encodes for the multidomain proteins WFIKKN1 and WFIKKN2, regulating the transforming growth factor beta (TGFB) superfamily [60]. The chemokine TGF-ß is a modulator of maternal immune tolerance in several species, including pigs [61,62,63].…”

Section: Discussionmentioning

confidence: 99%

Expression of Immune Regulatory Genes in the Porcine Internal Genital Tract Is Differentially Triggered by Spermatozoa and Seminal Plasma

Álvarez‐Rodríguez

Atikuzzaman

Venhoranta

et al. 2019

IJMS

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Mating or cervical deposition of spermatozoa or seminal plasma (SP) modifies the expression of genes affecting local immune defense processes at the oviductal sperm reservoir in animals with internal fertilization, frequently by down-regulation. Such responses may occur alongside sperm transport to or even beyond the reservoir. Here, immune-related gene expression was explored with cDNA microarrays on porcine cervix-to-infundibulum tissues, pre-/peri-ovulation. Samples were collected 24 h post-mating or cervical deposition of sperm-peak spermatozoa or SP (from the sperm-peak fraction or the whole ejaculate). All treatments of this interventional study affected gene expression. The concerted action of spermatozoa and SP down-regulated chemokine and cytokine (P00031), interferon-gamma signaling (P00035), and JAK/STAT (P00038) pathways in segments up to the sperm reservoir (utero-tubal junction (UTJ)/isthmus). Spermatozoa in the vanguard sperm-peak fraction (P1-AI), uniquely displayed an up-regulatory effect on these pathways in the ampulla and infundibulum. Sperm-free SP, on the other hand, did not lead to major effects on gene expression, despite the clinical notion that SP mitigates reactivity by the female immune system after mating or artificial insemination.

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WFIKKN1 and WFIKKN2: “Companion” proteins regulating TGFB activity

Cited by 15 publications

References 114 publications

A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions

A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions

Degenerative Suspensory Ligament Desmitis (DSLD) in Peruvian Paso Horses Is Characterized by Altered Expression of TGFβ Signaling Components in Adipose-Derived Stromal Fibroblasts

Expression of Immune Regulatory Genes in the Porcine Internal Genital Tract Is Differentially Triggered by Spermatozoa and Seminal Plasma

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