Water-soluble methacrylate as an embedding medium for the preparation of ultrathin sections

Rosenberg, Mark F.; Bartl, P.; Lesko, John J.

doi:10.1016/s0022-5320(60)80024-x

Cited by 101 publications

(23 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3D-F) closely mimicked the traditional procedure because of the exposure of samples to different resin concentrations and even to pure resin (O'Brien & McCully 1981;Gabriel 1982;Gerrits et al 1992). Gradual dehydration without the utilization of ethyl alcohol was initially used in studies aiming to improve infiltration methods for transmission electron microscopy (Stäubli 1963;Leduc & Bernhard 1967;Newman & Hobot 1999), in studies investigating the location of enzymes in animal and plant tissues (Rosenberg et al 1960;Leduc & Bernhard 1967;Ashford et al 1972;Newman & Hobot 1999), or for lipid preservation in animal tissues (Goor et al 1986;Meyer et al 2009). …”

Section: Resultsmentioning

confidence: 99%

“…Considering that diverse resins such as methacrylatebased resins, which are generally utilized for infiltration and embedding, are miscible in water in all proportions (Rosenberg et al 1960;Hayat 1970;O'Brien & McCully 1981), their use as a dehydration agent has been investigated (Rosenberg et al 1960;Hayat 1970;Ashford et al 1972;O'Brien & McCully 1981;Meyer et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Dehydration with resins has the advantages of simplifying the dehydration process, eliminating the use of ethanol or organic solvents and reducing artifacts such as lipid loss (Rosenberg et al 1960;Hayat 1970;O'Brien & McCully 1981). However, the use of resins for dehydration has been insufficiently applied for plant tissues and has only been utilized with the objective of enzyme preservation and respective detection by histochemical methods and not with the objective of lipid preservation (Ashford et al 1972).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simultaneous dehydration and infiltration with (2-hydroxyethyl)-methacrylate (HEMA) for lipid preservation in plant tissues

Oliveira

2015

Acta Bot. Bras.

View full text Add to dashboard Cite

Although this study aims to develop an improved method for the preservation of reserve lipids in plant tissues for different uses in plant anatomy, it mostly aims to develop an improved method for the identification of lipid reserves where synthesis or storage occurs. The proposed procedures entail only the utilization of (2-hydroxyethyl)-methacrylate (HEMA) as a dehydration agent. One of the procedures is based on the gradual exchange of aqueous HEMA solutions with increasing concentrations. In another procedure, dehydration and infiltration are induced by the presence of silica gel around a modified microcentrifuge tube containing the aqueous HEMA solution with the plant tissues, thus allowing efficient lipid preservation. Both procedures resulted in simultaneous dehydration and infiltration of the endosperm and embryo of Ricinus communis, while eliminating the use of ethyl alcohol, thus providing better lipid preservation.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous dehydration and infiltration with (2-hydroxyethyl)-methacrylate (HEMA) for lipid preservation in plant tissues

Oliveira

2015

Acta Bot. Bras.

View full text Add to dashboard Cite

show abstract

“…The time that sections remain in the reagent can also be problematic when using the wet chamber. Depending on the reaction time, the alcohol may generate swelling, degradation and detachment of the acrylic resin (Rosenberg et al 1960), leading to full or partial detachment of the sections (O'Brien & McCully 1981) distortions, folds or losses of parts of the tissues. The detachment of sections is a common problem in the procedure and it can be exacerbated by the method used to remove the reagent.…”

Section: Discussionmentioning

confidence: 99%

“…In both procedures it is essential to be careful with the sections during the separation of the histology slide from the device, because alcohol in the Sudan solution may cause sections to swell and detach (Rosenberg et al 1960;O'Brien & McCully 1981).…”

Section: Interruption Of the Reactionmentioning

confidence: 99%

How to construct and use a simple device to prevent the formation of precipitates when using Sudan Black B for histology

Oliveira

2015

Acta Bot. Bras.

View full text Add to dashboard Cite

The present work aims to demonstrate the stages of fabrication and use of a simple device to avoid the formation or fixation of precipitates from Sudan Black B solution on tissues. The device consists of four coverslip fragments attached to a histology slide, which serve as points of support for the histological slide under analysis. To work properly, the histology slide with the sections should be placed with the sections facing downwards the device. A small space between the device and the histology slide is thereby created by the height of the coverslip fragments. When Sudan Black B is applied, the solution is maintained within the edges of the device and evaporation is minimized by the small space, thereby reducing the consequent formation of precipitates. Furthermore, by placing the sections facing downward the device, any sporadically formed precipitates are prevented from settling on and fixing to the sectioned tissues or organs. By avoiding the formation of precipitates, plant cells, tissues and organs can be better observed, diagnosed and photomicrographically recorded.

show abstract

Synthesis, physical characterization, and biological performance of sequential homointerpenetrating polymer network sponges based on poly(2-hydroxyethyl methacrylate)

Lou

Vijayasekaran

Chirilă

et al. 1999

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

A limitation in the use of hydrophilic poly(2-hydroxyethyl methacrylate) (PHEMA) sponges as implantable devices is their inherently poor mechanical strength. This precludes proper surgical manipulation, especially in the eye where the size of the implant is usually small. In this study a new method was developed to produce mechanically stronger PHEMA sponges. Sequential homointerpenetrating polymer network (homo-IPN) sponges were made by using HEMA as the precursor for generating both the first network and the successive interpenetrated networks. Following the formation of network I, the sponge was squeezed to remove the interstitial water, soaked in the second monomer (also HEMA), and squeezed again to remove the excess monomer from the pores before being subjected to the second polymerization leading to the formation of network II. Two two-component IPN sponges (K2 and K4) with increasing HEMA content in the network II and a three-component IPN sponge (K3) were produced, and their properties were compared to those of a homopolymer PHEMA sponge (control). Apart from elongation, the tensile properties were all significantly enhanced in the IPN sponges; the water content was the same as in the control sponge, except for sponge K4, which was lower. Light microscopy revealed similar pore morphologies of the control and IPN sponges K2 and K3, and the majority of the pores were around 25 microm. Sponge K4 displayed smaller pores of around 10 microm. Cellular invasion into the sponges was examined in vitro (incubation with 3T3 fibroblasts) and in vivo (implantation in rabbit corneas). Although the in vitro assay detected a change in the cell behavior in the early stage of invasion, which was probably due to the formation of IPNs, such changes were not reflected in the longer term in vivo experiment. There was a proper integration of sponges K2 and K3 with the corneal stroma, but much less cellular invasion and no neovascularization in sponge K4. We concluded that IPN formation is a valid method to enhance the strength of PHEMA sponges, provided that the content of HEMA in the successive networks is not too high.

show abstract

Water-soluble methacrylate as an embedding medium for the preparation of ultrathin sections

Cited by 101 publications

References 6 publications

Simultaneous dehydration and infiltration with (2-hydroxyethyl)-methacrylate (HEMA) for lipid preservation in plant tissues

Simultaneous dehydration and infiltration with (2-hydroxyethyl)-methacrylate (HEMA) for lipid preservation in plant tissues

How to construct and use a simple device to prevent the formation of precipitates when using Sudan Black B for histology

Synthesis, physical characterization, and biological performance of sequential homointerpenetrating polymer network sponges based on poly(2-hydroxyethyl methacrylate)

Contact Info

Product

Resources

About