Water-soluble covalent conjugates of bovine serum albumin with anionic poly(N-isopropyl-acrylamide) and their immunogenicity

Dilgimen, Aydan Salman; Mustafaeva, Zeynep; Demchenko, M. O.; Kaneko, Tatsuo; Osada, Yoshihito; Mustafaev, Mamed

doi:10.1016/s0142-9612(00)00425-7

Cited by 54 publications

(25 citation statements)

References 16 publications

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“…However, gel-permeating chromatography has been a widely used technique for estimating these characteristics of proteins, polysaccharides, and protein-polymer conjugates in their native forms based on their elution positions [28,29]. The size exclusion chromatograms of the enzyme-dextran Fig.…”

Section: Resultsmentioning

confidence: 99%

Glucose Oxidase-dextran Conjugates with Enhanced Stabilities Against Temperature and pH

Altıkatoğlu

Basaran

Ariöz

et al. 2010

Appl Biochem Biotechnol

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Multipoint covalent bonding of glucose oxidase (EC 1.1.3.4) to hydrophilic natural polymer dextran and optimization of procedures to obtain, with enhanced temperature and pH stabilities, were studied. Purified enzyme was conjugated with various molecular weight dextrans (17.5, 75, and 188 kD) in a ratio of 20:1, 10:1, 1:1, 1:5, 1:10, 1:15, and 1:20. After 1 h of incubation at pH 7, the activities of purified enzyme and conjugates were determined at different temperatures (25 degrees C, 30 degrees C, 35 degrees C, 40 degrees C, 50 degrees C, 60 degrees C, 70 degrees C, and 80 degrees C), and the results were evaluated for thermal resistance. Increases in temperature from 25 degrees C to 50 degrees C did not change the activities of the conjugates. The conjugate, which was prepared with 75 kDa dextran in a molar ratio of 1:5, showed the highest thermal resistance and even the activity still remains at 80 degrees C at pH 7.0. This conjugate also displayed activity in a wide pH range (pH 4.0-7.0) at high temperatures. Conjugate, which was synthesized with 75 kDa dextran in a molar ratio of 1:5, appears to be feasible and useful for biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

Glucose Oxidase-dextran Conjugates with Enhanced Stabilities Against Temperature and pH

Altıkatoğlu

Basaran

Ariöz

et al. 2010

Appl Biochem Biotechnol

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“…Carbodiimide has been widely used as carboxyl-and amine-reactive crosslinker to prepare immunoliposomes (Endoh et al, 1981), polymer-protein conjugates (Dilgimen et al, 2001) and immunomicrospheres (MacAdam et al, 2000). However, the pH remote loading method is not available for DXR encapsulation into the albumin-conjugated PEG liposome, since carbodiimide can activate carbonyl group only at a low pH region, especially between 3.5 and 4.5 during the first step of reaction (Nakajima and Ikeda, 1995).…”

Section: Discussionmentioning

confidence: 99%

Albumin-conjugated PEG liposome enhances tumor distribution of liposomal doxorubicin in rats

Yokoe

Sakuragi

Yamamoto

et al. 2008

International Journal of Pharmaceutics

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To evaluate the effect of coupling of recombinant human serum albumin (rHSA) onto the surface of poly(ethylene glycol)-modified liposome (PEG liposome) on the in-vivo disposition characteristics of liposomal doxorubicin (DXR), the pharmacokinetics and tissue distribution of DXR were evaluated after intravenous administration of rHSA-modified PEG (rHSA/PEG) liposomal DXR into tumor-bearing rats.rHSA/PEG liposome prepared using a hetero-bifunctional cross-linker, N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP), efficiently encapsulated DXR (over 95%). rHSA/PEG liposomal DXR showed longer blood-circulating property than PEG liposomal DXR and the hepatic and splenic clearances of rHSA/PEG liposomal DXR were significantly smaller than those of PEG liposomal DXR. It was also demonstrated that the disposition of DXR to the heart, one of the organs for DXR-related side-effects, was significantly smaller than free DXR. Furthermore, the tumor accumulation of rHSA/PEG liposomal DXR was significantly larger than that of PEG liposomal DXR. The "therapeutic index", a criterion for therapeutic outcome, for rHSA/PEG liposomal DXR was significantly higher than PEG liposomal DXR. These results clearly indicate that rHSA-conjugation onto the surface of PEG liposome would be a useful approach to increase the effectiveness and safety of PEG liposomal DXR.

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“…Volume fraction of NIPAM in each copolymer was estimated from x and the densities of the pair of comonomers. 8.4 g of the (mixed) monomer, 0.05 g of AIBN and 0.01g of MPA was dissolved separately in 50 cm 3 of ethanol (total volume). MPA was used for attaching a carboxylic group at the end of the copolymers.…”

Section: Preparation Of Samplesmentioning

confidence: 99%

“…Fujishige et al reported a change in comformation of individual PNIPAM chains from random coil to compact globule followed by an inter-chain aggregation due to the phase separation [2]. The phase separation on heating is believed as a phase behavior with the lower critical solution temperature (LCST) [3] which is common to all polymer solutions, although it is stilll controversial [4]. This phase transition of PNIPAM has been used in attempts to control the enzymatic activity [5][6][7][8][9], affinity precipitation separation [10,11] and protein recycling systems [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and phase behavior of aqueous poly(N-isopropylacrylamide-co-acrylamide), poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) and poly(N-isopropylacrylamide-co-2-hydroxyethyl methacrylate)

et al. 2006

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Poly(N-isopropylacrylamide) (PNIPAM) and random copolymers of Poly(N-isopropylacrylamide-co-2-hydroxyethyl methacrylate) (PNIPAM-HEMA), poly(N-isopropylacrylamide-co-acrylamide) (PNIPAM-AAm) and poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) (PNIPAM-DMAA) with various volume fractions of NIPAM , were synthesized by radical polymerization. The phase behavior of the polymers in water was investigated by means of optical transmittance and dynamic light scattering. With decreasing , the cloud point temperature T cp for PNIPAM-HEMA decreased whereas T cp for both PNIPAM-AAm and PNIPAM-DMAA increased. Increase of hydrodynamic radius around T cp resulted from the aggregation of the globules of each polymer was observed from dynamic light scattering. The relationships between the reciprocal of T cp of the polymer solutions and 1-were linear for the three copolymers in the experimental range of 0.65 < < 1. The results are discussed from the aspect of the interaction parameters of copolymer solutions.

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Water-soluble covalent conjugates of bovine serum albumin with anionic poly(N-isopropyl-acrylamide) and their immunogenicity

Cited by 54 publications

References 16 publications

Glucose Oxidase-dextran Conjugates with Enhanced Stabilities Against Temperature and pH

Glucose Oxidase-dextran Conjugates with Enhanced Stabilities Against Temperature and pH

Albumin-conjugated PEG liposome enhances tumor distribution of liposomal doxorubicin in rats

Synthesis and phase behavior of aqueous poly(N-isopropylacrylamide-co-acrylamide), poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) and poly(N-isopropylacrylamide-co-2-hydroxyethyl methacrylate)

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