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Németh, Áron; Kupcsulik, B.; Sevella, B.

doi:10.1023/a:1025116308484

Cited by 39 publications

(9 citation statements)

References 16 publications

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“…To prevent possible over-acidification, 100 mM of 2-(N-morpholino)ethanesulfonic acid (MES) [16] was added into the M-CAB medium [17]. K. pneumoniae DSM 2026 and K. pneumoniae DSM 4799 were grown in production medium, which contained the following components per liter of distilled water: 5 g of K 2 HPO 4 , 3 g of KH 2 PO 4 , 2 g of (NH 4 ) 2 SO 4 , 0.4 g of MgSO 4 ·7H 2 O, 0.1 g of CaCl 2 ·2H 2 O, 2 g of yeast extract, 0.5 g of peptone, 0.3 g of beef extract, and 1 mL of trace elements [18].…”

Section: Methodsmentioning

confidence: 99%

Effect of Biodiesel-derived Raw Glycerol on 1,3-Propanediol Production by Different Microorganisms

Moon

Ahn

Kim

et al. 2009

Appl Biochem Biotechnol

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The microbial production of 1,3-propanediol (1,3-PD) from raw glycerol, a byproduct of biodiesel production, is economically and environmentally advantageous. Although direct use of raw glycerol without any pretreatment is desirable, previous studies have reported that this could cause inhibition of microbial growth. In this study, we investigated the effects of raw glycerol type, different microorganisms, and pretreatment of raw glycerol on the production of 1,3-PD. Raw glycerol from waste vegetable-oil-based biodiesel production generally caused more inhibition of 1,3-PD production and microbial growth compared to raw glycerol from soybean-oil-based biodiesel production. In addition, two raw glycerol types produced from two biodiesel manufacturers using waste vegetable oil exhibited different 1,3-PD production behavior, partially due to different amounts of methanol included in the raw glycerol from the two biodiesel manufacturers. Klebsiella strains were generally resistant to all types of raw glycerol while the growth of Clostridium strains was variably inhibited depending on the type of raw glycerol. The 1,3-PD production of the Clostridium strains using acid-pretreated raw glycerol was significantly enhanced compared to that with raw glycerol, demonstrating the feasibility of using raw glycerol for 1,3-PD production by various microorganisms.

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Section: Methodsmentioning

confidence: 99%

Effect of Biodiesel-derived Raw Glycerol on 1,3-Propanediol Production by Different Microorganisms

Moon

Ahn

Kim

et al. 2009

Appl Biochem Biotechnol

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“…A number of microorganisms are able to grow anaerobically on glycerol as the sole carbon and energy source, such as Citrobacter freundii [39,40], Klebsiella pneumoniae [41,42], Clostridium butyricum [43,44], Enterobacter agglomerans [45,46], Enterobacter aerogenes [47] and Lactobacillus reuteri [48]. However, most of them do not convert this substrate into butanol.…”

Section: Microbial Metabolism Of Glycerolmentioning

confidence: 99%

A Review of Process-Design Challenges for Industrial Fermentation of Butanol from Crude Glycerol by Non-Biphasic Clostridium pasteurianum

et al. 2016

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Butanol, produced via traditional acetone-butanol-ethanol (ABE) fermentation, suffers from low yield and productivity. In this article, a non-ABE butanol production process is reviewed. Clostridium pasteurianum has a non-biphasic metabolism, alternatively producing 1,3-propanediol (PDO)-butanol-ethanol, referred to as PBE fermentation. This review discusses the advantages of PBE fermentation with an emphasis on applications using biodiesel-derived crude glycerol, currently an inexpensive and readily available feedstock. To address the process design challenges, various strategies have been employed and are examined and reviewed; genetic engineering and mutagenesis of C. pasteurianum, characterization and pretreatment of crude glycerol and various fermentation strategies such as bioreactor design and configuration, increasing cell density and in-situ product removal. Where research deficiencies exist for PBE fermentation, the process solutions as employed for ABE fermentation are reviewed and their suitability for PBE is discussed. Each of the obstacles against high butanol production has multiple solutions, which are reviewed with the end-goal of an integrated process for continuous high level butanol production and recovery using C. pasteurianum and biodiesel-derived crude glycerol.

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“…pneumoniae DSM 4799 was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Branunschweig, Germany). The preculture medium contained the following (per liter): peptone 5 g and beef extract 3 g. A fermentation medium for 1,3-PD production contained (per liter): K 2 HPO 4 5 g, KH 2 PO 4 3 g, (NH 4 ) 2 SO 4 2 g, MgSO 4 ·7H 2 O 0.4 g, CaCl 2 ·2H 2 O 0.1 g, yeast extract 2 g, peptone 0.5 g, beef extract 0.3 g (Difco Laboratories, Becton, Dickinson & Co., USA), and trace element solutions 1 mL [11]. The carbon sources used were pure glycerol (J. T. Baker, 99.9%) and raw glycerol (Nexenco, Korea), which was a main by-product (purity 80% w/w) from the methanolysis of soybean oil using an alkali-catalyzed process.…”

Section: Microorganism and Mediummentioning

confidence: 99%

Microbial Fed-batch Production of 1,3-Propanediol Using Raw Glycerol with Suspended and Immobilized Klebsiella pneumoniae

Jun

Moon

Kang

et al. 2009

Appl Biochem Biotechnol

107

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The production of 1,3-propanediol (1,3-PD) was investigated with Klebsiella pneumoniae DSM 4799 using raw glycerol without purification obtained from a biodiesel production process. Fed-batch cultures with suspended cells revealed that 1,3-PD production was more effective when utilizing raw glycerol than pure glycerol (productivity after 47 h of fermentation, 0.84 g L(-1) 1 h(-1) versus 1.51 g L(-1) h(-1) with pure and raw glycerol,respectively). In addition, more than 80 g/L of 1,3-PD was produced using raw glycerol;this is the highest 1,3-PD concentration reported thus far for K. pneumoniae using raw glycerol. Repeated fed-batch fermentation with cell immobilization in a fixed-bed reactor was performed to enhance 1,3-PD production. Production of 1,3-PD increased with the cycle number (1.06 g L(-1) h(-1) versus 1.61 g L(-1) h(-1) at the first and fourth cycle, respectively)due to successful cell immobilization. During 46 cycles of fed-batch fermentation taking place over 1,460 h, a stable and reproducible 1,3-PD production performance was observed with both pure and raw glycerol. Based on our results, repeated fed batch with immobilized cells is an efficient fermentor configuration, and raw glycerol can be utilized to produce 1,3-PD without inhibitory effects caused by accumulated impurities.

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Untitled

Cited by 39 publications

References 16 publications

Effect of Biodiesel-derived Raw Glycerol on 1,3-Propanediol Production by Different Microorganisms

Effect of Biodiesel-derived Raw Glycerol on 1,3-Propanediol Production by Different Microorganisms

A Review of Process-Design Challenges for Industrial Fermentation of Butanol from Crude Glycerol by Non-Biphasic Clostridium pasteurianum

Microbial Fed-batch Production of 1,3-Propanediol Using Raw Glycerol with Suspended and Immobilized Klebsiella pneumoniae

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