Volvopluteus and Pluteus section Pluteus (Agaricales: Pluteaceae) in Turkey based on morphological and molecular data

Abstract

Pluteus and Volvopluteus are two closely related genera of Pluteaceae that comprise saprotrophic fungi. Although the genera have been well-studied in Europe, North and South America, the diversity of Pluteus, mainly Pluteus sect. Pluteus, and Volvopluteus in Turkey is poorly known due to insufficient morphological descriptions and a lack of DNA sequence data. In this study, the diversity of Pluteus sect. Pluteus and Volvopluteus in Turkey is evaluated by employing both morphological features and phylogenetic approaches. A general phylogeny of Pluteus sect. Pluteus based on sequences of nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) region is presented and four species are given as new records for Turkey: P. brunneidiscus, P. hongoi, P. pellitus, and P. primus. According to the results of the multi-gene molecular analyses (ITS, 28S = nrLSU, and 18S = nrSSU datasets) of the genus Volvopluteus, the occurrence of Volvopluteus earlei is reported for the first time in Turkey. Besides, the ITS sequences data of the Turkish collections of P. atromarginatus, P. cervinus, P. kovalenkoi, P. petasatus, P. pouzarianus, P. salicinus, P. shikae, and ITS, 28S and 18S data for V. gloiocephalus are given for the first time. The detailed descriptions, colour photographs, and microscopic drawings of the newly recorded species are provided, and their comparisons with morphological and phylogenetically similar species are discussed.

Holarctic Species in the Pluteus romellii Clade. Five New Species Described and Old Names Reassessed

“…The type material of P. pallescens , deposited in K, was studied by B. Dima, and the molecular methods followed [ 65 , 66 ]. The total genomic DNA of OK collections was extracted in accordance with Kaygusuz et al [ 67 ]. For collections in LE, the DNA extraction procedure completely corresponded to the manufacturing protocol of the Phytosorb Kit (ZAO Syntol).…”

Callistosporium luteo-olivaceum (Callistosporiaceae: Basidiomycota), an agaric fungi new to Türkiye

“…The genomic DNA was extracted from dried specimens using ZR Fungal/Bacterial DNA MiniPrep kit (Zymo research, Irvine, California) according to the manufacturer's protocol. Polymerase chain reaction was performed as described by Kaygusuz et al (2021) using the primer pairs ITS1F/ITS4 (White et al, 1990) and LR0R/LR5 (Vilgalys and Hester, 1990) to amplify nrITS and nLSU gene regions, respectively. Same primer sets were used for sequencing reactions (Source Bioscience, Berlin, Germany).…”

Xerophorus donadinii (Bon) Vizzini, Consiglio & M. Marchetti (Callistosporiaceae: Agaricales) from Mediterranean Region of Turkey

“…Microscopic structures were observed in dried material stained in ammoniacal Congo red. The following abbreviations are used: L m and W m indicate the average length and width of basidiospores, Q shows the ratios of length/width and Q m presents the average quotient of the measured basidiospores [5]. Specimens are kept at the fungarium of Isparta University of Applied Sciences, Isparta, Turkey.…”

“…DNA isolation, polymerase chain reaction (PCR) amplifications and sequencing methods were carried out according to Kaygusuz et al [5]. The primer pairs ITS1F/ITS4 [6,7] and LR0R/LR5 [8] were used for amplifying nrITS and nrLSU gene regions, respectively.…”