Vitreous humor endogenous compounds analysis for post-mortem forensic investigation

Pigaiani, Nicola; Bertaso, Anna; Palo, Elio Franco De; Bortolotti, Federica; Tagliaro, Franco

doi:10.1016/j.forsciint.2020.110235

Cited by 48 publications

(69 citation statements)

References 187 publications

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“…acetate and dimethylsulfone). Decreasing potassium errors are in line with what is observed with vitreous potassium, where PMI extrapolation errors were observed to decrease with increasing PMI, indicating increased suitability for forensic casework analysis later than the 24-hperiod after death [4,12].…”

Section: Discussionsupporting

confidence: 82%

“…( 2 ) resulted in the model with the best regression parameters, which are reported in Table 2 . For potassium behaviour in vitreous humour, the majority of the relevant literature proposed a linear model but even a nonlinear one was suggested as a function of PMI, with a faster rise in the first few hours than after 24 h after death [ 4 ]. The predictive ability of the AH potassium model calculated by the use of the test set samples indicated that potassium is less performant than the 1 H NMR metabolomic profile for PMI estimation, since the errors in prediction over the 24-h period were 210 versus 99 min, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Traditional biochemical methods have been applied to identify metabolite changes after death in several biofluids [1][2][3]. Vitreous humour (VH) is the most studied fluid for post-mortem analysis given its correlation with ante-mortem serum composition and its longer stability compared with blood [4]. Potassium was the VH analyte most deeply investigated, and its time-dependent postmortem increase is currently considered the most promising experimental predictor for PMI estimation [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model

et al. 2020

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Estimation of the post-mortem interval (PMI) remains a matter of concern in the forensic scenario. Traditional and novel approaches are not yet able to fully address this issue, which relies on complex biological phenomena triggered by death. For this purpose, eye compartments may be chosen for experimental studies because they are more resistant to post-mortem modifications. Vitreous humour, in particular, has been extensively investigated, with potassium concentration ([K+]) being the marker that is better correlated with PMI estimation. Recently, a 1H nuclear magnetic resonance (NMR) metabolomic approach based on aqueous humour (AH) from an animal model was proposed for PMI estimation, resulting in a robust and validated regression model. Here we studied the variation in [K+] in the same experimental setup. [K+] was determined through capillary ion analysis (CIA) and a regression analysis was performed. Moreover, it was investigated whether the PMI information related to potassium could improve the metabolome predictive power in estimating the PMI. Interestingly, we found that a part of the metabolomic profile is able to explain most of the information carried by potassium, suggesting that the rise in both potassium and metabolite concentrations relies on a similar biological mechanism. In the first 24-h PMI window, the AH metabolomic profile shows greater predictive power than [K+] behaviour, suggesting its potential use as an additional tool for estimating the time since death.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model

et al. 2020

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“…The highest concentrations are found in blood collected from the left heart, vena cava or hepatic vein, most likely due to hepatic glycogenolysis [14,16]. Because of this, other fluids have been proposed for glucose determination such as vitreous humor (VH) and cerebrospinal fluid (CSF) [14,[16][17][18][19][20]. VH is considered the fluid of choice, being the most protected site against postmortem changes, with the lowest rate of glycolysis.…”

Section: Discussionmentioning

confidence: 99%

“…The results are presented in Table 1. [16][17][18][19][20] and ketone bodies value increase five times above the normal threshold (normal values differ depending on the method of determination); for the colorimetric method, using the MAK041 kit, the diagnostic threshold value is 0.5 mmol/L [28]; for the point-of-care system XPER Technology, the diagnostic threshold value is 2.5 mmol/L [14,[16][17][18][19][20][21][22][23][24].…”

Section: Hba1c Methodsmentioning

confidence: 99%

The Vital Role of Thanatochemistry in the Postmortem Diagnostic of Diabetic Ketoacidosis—Case Report

et al. 2021

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Diabetic ketoacidosis (DKA) is a lethal acute hyperglycemic complication of diabetes mellitus (DM) and it represents the initial manifestation of DM in about 15–20% of cases in adults and about 30–40% of cases in children. Postmortem diagnosis of DKA can only be made by applying thanatochemistry. Biochemistry applied postmortem is viewed with skepticism by many practitioners in the forensic field, completely lacking in many forensic services around the world, and especially in the national ones. This article aims to underline the importance of the postmortem application of biochemistry by reviewing the case of a person in the third decade of life who died suddenly at home due to diabetic ketoacidosis (DKA), whose autopsy was performed at an early PMI of approximately 24 h. Routine postmortem examinations (macroscopic, anatomopathological, and toxicological) could not establish a clear cause of death. When attention was turned to biochemical determinations (i.e., determination of glycated hemoglobin, glucose and ketone bodies (acetone, beta-hydroxybutyrate) in the blood, vitreous humor, and cerebrospinal fluid), the identified values clarified the thanatogenic mechanisms by establishing the diagnosis of DKA.

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Unrevealing the connection between real sample analysis and analytical method. The case of cytokines

Irimes

Tertiş

Oprean

et al. 2023

Medicinal Research Reviews

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Cytokines are compounds that belong to a special class of signaling biomolecules that are responsible for several functions in the human body, being involved in cell growth, inflammatory, and neoplastic processes. Thus, they represent valuable biomarkers for diagnosing and drug therapy monitoring certain medical conditions. Because cytokines are secreted in the human body, they can be detected in both conventional samples, such as blood or urine, but also in samples less used in medical practice such as sweat or saliva. As the importance of cytokines was identified, various analytical methods for their determination in biological fluids were reported. The gold standard in cytokine detection is considered the enzyme‐linked immunosorbent assay method and the most recent ones have been considered and compared in this study. It is known that the conventional methods are accompanied by a few disadvantages that new methods of analysis, especially electrochemical sensors, are trying to overcome. Electrochemical sensors proved to be suited for the elaboration of integrated, portable, and wearable sensing devices, which could also facilitate cytokines determination in medical practice.

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Vitreous humor endogenous compounds analysis for post-mortem forensic investigation

Cited by 48 publications

References 187 publications

Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model

Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model

The Vital Role of Thanatochemistry in the Postmortem Diagnostic of Diabetic Ketoacidosis—Case Report

Unrevealing the connection between real sample analysis and analytical method. The case of cytokines

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