Vitreous aspiration needle tap in the diagnosis of intraocular inflammation

Lobo, Aires; Lightman, Susan

doi:10.1016/s0161-6420(02)01895-x

Cited by 85 publications

(45 citation statements)

References 21 publications

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“…Some of the intraocular cells may be dead or damaged in transit or have poor cytomorphology. Direct aspiration through 21-gauge large bore needles, 42 and vitrectomy with 20-gauge vitrectors 9 or 25-gauge vitrectors 43 produces adequate specimens for morphologic diagnosis. Rapid processing within 1 h after acquisition has been recommended.…”

Section: Diagnosis Of Intraocular Lymphomamentioning

confidence: 99%

Intraocular lymphoma: a clinical perspective

Davis

2012

Eye

113

121

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Section: Diagnosis Of Intraocular Lymphomamentioning

confidence: 99%

Intraocular lymphoma: a clinical perspective

Davis

2012

Eye

113

121

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“…[4][5][6][7]9,10,20,21,26,[30][31][32][33][34][35] These include: (1) vitreous 'filtration'; (2) a celloidin bag technique; (3) cytospin and (4) cell block preparation. We are most familiar with the latter two methods, whereby the vitreous is spun at 500 r.p.m.…”

Section: Sample Handling and Processingmentioning

confidence: 99%

The pathologist's perspective on vitreous opacities

Coupland

2008

Eye

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“…Data on complications of vitreous biopsy are sparse, but one series of 53 cases reported one occurrence of retinal detachment. 11 PPV requires more specialised equipment and training, and takes longer to perform. The potential complications of PPV are well understood and include cataract, raised intraocular pressure and retinal tears or detachment.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic cellular yield is superior with full pars plana vitrectomy compared with core vitreous biopsy

Mudhar

Sheard

2012

Eye

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Purpose Vitreous biopsy for the cytological assessment of suspected intraocular lymphoma and vitritis of uncertain aetiology is a standard investigation. The types of specimens generated and the diagnostic rate are variable within and between centres. There are many reasons for this but one observation that has not been considered previously is the differential distribution of cells in the vitreous gel. To test this possibility, five consecutive patients with suspected vitreous involvement by lymphoma or vitritis of uncertain aetiology underwent a core vitreous biopsy immediately before a planned full pars plana vitrectomy (PPV) and the cellularity of the two sampling techniques compared. Methods A prospective study of five consecutive patients requiring vitreous sampling to secure a firm diagnosis. For each of five patients, the core vitreous biopsy specimen was received in a universal tube and the PPV specimen was received in a vitreous cassette. Fluid (0.25 ml) was removed from both specimens, centrifuged and haematoxylin and eosin (H&E) stained slides prepared per sampling method. The slides were examined with a light microscope, the most cellular field selected and the number of cells per mm 2 counted and compared between sampling techniques. Results PPV specimen's, revealed a cellularity range that was 7.4 to 78 Â (average 31 Â ) greater than a core vitreous biopsy. In the two cases of a final diagnosis of intraocular lymphoma, the vitreous core biopsy was non-diagnostic. Furthermore, the PPV specimen generated additional cellular material for numerous ancillary investigations to permit a secure diagnosis. Conclusions The results of this differential vitreous sampling study has strengthened our anecdotal slit lamp clinical observations that inflammatory cells and lymphoma cells are concentrated more in the cortical vitreous. Therefore, vitreous cells have less chance to be sampled if a single core vitreous biopsy is performed. Indeed, the two cases of confirmed lymphoma generated a non-diagnostic core vitreous biopsy. In our centre, this study has lead to PPV being performed as a gold standard on all patients with suspected intraocular lymphoma or vitritis of uncertain aetiology.

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Vitreous aspiration needle tap in the diagnosis of intraocular inflammation

Cited by 85 publications

References 21 publications

Intraocular lymphoma: a clinical perspective

Intraocular lymphoma: a clinical perspective

The pathologist's perspective on vitreous opacities

Diagnostic cellular yield is superior with full pars plana vitrectomy compared with core vitreous biopsy

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