Vitamin D is associated with metabotropic but not neurotrophic effects of exercise in ovariectomized rats

Abstract

PurposeHere, we studied the beneficial effects of aerobic exercise on metabolic syndrome components, cognitive performance, brain derived neurotrophic factor (BDNF) and irisin in ovariectomized rats with different serum vitamin D (Vit D) status.MethodsEighty female wistar rats were divided into 2 groups of sham operated (sham, n = 8), and ovariectomized (OVX, n = 72). Then OVX were divided into 9 groups of receiving combination of exercise protocol with low dose of Vit D (OVX + EXE + LD), high dose of Vit D (OVX + EXE + HD), Vit D deficiency (OVX + EXE − D), and (OVX + EXE + Veh). Also non exercised groups of OVX receiving high dose of Vit D (OVX + HD), low dose of Vit D (OVX + LD), Vit D deficiency (OVX − D), and Veh (OVX + Veh) were included. After 2 months of related interventions, spatial memory was assessed using Morris water maze (MWM), and then metabolic syndrome components were measured.ResultsHigh dose of Vit D supplementation showed significant reduction in weight (p = 0.001), lipid profiles (p = 0.001), visceral fat (p = 0.001) and waist circumference (p = 0.001) regardless of exercising or not, with no change in cognitiive function. Serum BDNF level was significantly higher in Vit D deficient group (p = 0.001), and was decreased in the OVX + HD. In contrary, irisin did not show any significant relationship with serum concentration of Vit D, while it was significantly elevated in the exercised groups compared with non-exercised counterparts.ConclusionVit D insufficiency deteriorates metabolic syndrome components, and elevates serum BDNF as a compensatory metabotropic factor, and further supplementation significantly attenuates these components parallel with reduction in BDNF. In addition, aerobic exercise successfully induces various metabolic benefits, provided optimum serum level of Vit D.

Vitamin D Replacement Mitigates Menopause-Associated Dyslipidaemia and Atherogenic Indices in Ovariectomized Rats; A Biochemical Study

“…Also, seasonal variations of sunlight ultraviolet exposure and increasing indoor activities reduced cutaneous synthesis of VD [33]. In line with this finding, Babaei et al reported VD insufficiency in ovariectomized rats [34].…”

Association of Circulating 25-Hydroxyvitamin D Levels with Colorectal Cancer: An Updated Meta-Analysis

“…Among these, difference in physical activity was the most obvious (p50.002) and showed the least heterogeneity (I 2 50). After selection of supporting evidence, we found that aerobic exercise could successfully induce a variety of metabolic activities, which adjusted serum vitamin D into an optimal level (44). At the same time, studies in Japan found that endurance training suppressed a seasonal decrease in serum 25(OH)D concentrations (45).…”

Flavonoids Fraction of Mespilus Germanica Alleviates Insulin Resistance in Metabolic Syndrome Model of Ovariectomized Rats via Reduction in Tumor Necrosis Factor-α

“…Animals were injected intraperitoneally combination of 75 mg/kg ketamine (50 mg/mL; Rotexmedica GmbH, Trittau, Germany) and 5 mg/kg of xylazine (20 mg/mL; ScienceLab, Houston, TX, USA). After complete anesthesia, ovaries were accurately removed through midline incision 21…”

“…Body weight, Wc and visceral fats were measured after 14 hours fasting. Wc was measured on the largest zone of the rat abdomen 21. Visceral fats were isolated from surrounding tissues of mesenteric, urogenital and retroperitoneal regions and weighed using a weighing-scale (Doulton) 21.…”