Vitamin B12: Strategies for enhanced production, fortified functional food products and health benefits

Kumar, Raju Suresh; Singh, Umesh; Tiwari, Anand P.; Tiwari, Pooja; Sahu, Jatindra K.; Sharma, Satyawati

doi:10.1016/j.procbio.2023.02.002

Cited by 10 publications

(4 citation statements)

References 105 publications

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“…A GC-MS analysis of the BC Cyclo extract allow us to highlight the presence of fatty acids (23,32,34,39,42,44,46,47) and fatty aldehyde (36 ′ , 44 ′ ). Despite the absence of a conclusion on the polyphenol production by B. megaterium through the Folin-Ciocalteu method, polyphenol profiles by GC-MS evolved after B. megaterium production (21,22,37,43,53,58). These results confirm previous internals results which showed that B. megaterium produces several phenols [29].…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, B. megaterium has already been commercialized for agricultural applications through its inoculation as a living organism [17]. But few B. megaterium metabolites have been described so far (phytohormones [20], antibiotic lipopeptides [21], vitamin B12 [22], siderophores [23], biopolymer [18], carotenoids [23], exopolysaccharides [24]) and valuated through their direct utilization for agriculture industry. In the present work, in order to identify its secondary metabolites related to plant growth promotion, a composition and activity analysis of B. megaterium filtrated supernatant was achieved through extraction, chemical family quantification, high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), antioxidant analysis and seed germination.…”

Section: Introductionmentioning

confidence: 99%

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Bacillus megaterium: Evaluation of Chemical Nature of Metabolites and Their Antioxidant and Agronomics Properties

Hur,

Saoudi,

Ferhout

et al. 2024

IJMS

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Bacillus megaterium is particularly known for its abundance in soils and its plant growth promotion. To characterize the metabolites excreted by this specie, we performed successive liquid/liquid extractions from bacteria culture medium with different polarity solvents (cyclohexane, dichloromethane, ethyl acetate and butanol) to separate the metabolites in different polarity groups. The extracts were characterized regarding their total phenolic content, the amount of reducing sugar, the concentration of primary amines and proteins, their chromatographic profile by HPLC-DAD-ELSD and their chemical identification by GC-MS. Among the 75 compounds which are produced by the bacteria, 19 identifications were for the first time found as metabolites of B. megaterium and 23 were described for the first time as metabolites in Bacillus genus. The different extracts containing B. megaterium metabolites showed interesting agronomic activity, with a global inhibition of seed germination rates of soya, sunflower, corn and ray grass, but not of corn, compared to culture medium alone. Our results suggest that B. megaterium can produce various metabolites, like butanediol, cyclic dipeptides, fatty acids, and hydrocarbons, with diverse effects and sometimes with opposite effects in order to modulate its response to plant growth and adapt to various environmental effects. These findings provide new insight into bioactive properties of this species for therapeutic uses on plants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacillus megaterium: Evaluation of Chemical Nature of Metabolites and Their Antioxidant and Agronomics Properties

Hur,

Saoudi,

Ferhout

et al. 2024

IJMS

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show abstract

“…When the content of target compounds such as vitamins is complex, it is sometimes necessary to use multiple techniques to analyze the active ingredients of these vitamins. Traditional detection methods include microbiological methods [24], electrochemical methods [25], capillary electrophoresis [26], thin-layer chromatography [27], etc. However, traditional methods cannot meet the requirements of modern detection for simultaneous detection of multiple vitamins.…”

Section: Growth and Reproduction Of Organism Cellsmentioning

confidence: 99%

Research progress on the detection of water-soluble vitamins in food using liquid chromatography

Zhang,

Zhang

2024

TNS

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Currently, liquid chromatography is widely used for the analysis and determination of water-soluble vitamins (WSVs) in functional foods. However, due to the diversity of sample characteristics and the requirement for purification accuracy, the chromatographic conditions and specific methods used vary. This paper reviews the chromatographic conditions of liquid chromatography techniques for detecting WSVs in food, as well as the selection of liquid chromatography methods in different application fields. The chromatographic conditions include chromatographic columns, column temperature, mobile phase, flow rate, and elution gradient, and the application scope of different chromatographic conditions is compared. Liquid chromatography methods include high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry, and discussions are made on the applications and prospects of these two methods under different chromatographic conditions. Therefore, this review provides research insights into different chromatographic conditions and methods for food quality inspection and biochemical analysis research.

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“…Besides, this product has a low content of fat (2 g per serving), making it a great option for consumers looking for a lean protein source. Additionally, tuna mojama contains important minerals, such as iron and calcium, and it is a good source of vitamin B12, an essential nutrient that helps maintain healthy nerve cells and red blood cells and it is involved in DNA synthesis [9,10]. The value of this product was recognized in 2015 by the EU with the registration of two Protected Geographical Indications (PGI), namely "Mojama de Barbate" [11,12] (Cádiz, Spain) and "Mojama de Isla Cristina" (Huelva, Spain) [13,14] in the Andalusia region.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical and Microbiological Changes Associated with Processing in Dry-Cured Tuna

et al. 2023

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Dry-cured tuna is a traditional product manufactured in the Mediterranean region of Spain, known as mojama. The traditional salting-drying elaboration process attributes new organoleptic characteristics to the final product, changing its flavor, color, and nutritional value. This study aimed to evaluate the changes in physicochemical, biochemical, and microbiological parameters taking place during the process. The physicochemical parameters were affected by the processing steps (salting, salt-washing, and drying), except for total acidity and pH. The water activity value and relative moisture percentage decreased to 0.86 and 33.03%, respectively. Moreover, the addition of salt and the drying step increased the water-holding capacity. The lipid oxidation values increased from raw tuna loins to the final product (1.37 vs. 5.56 mg malondialdehyde/kg). Moreover, the total volatile basic nitrogen values increased in the final product, fundamentally due to the concentration effect caused by the water loss, although may also be due to the degradation of proteins during processing. The microbiological analysis showed that the values obtained in the dry-cured tuna were below the limits established by the reference regulation for dry-cured fish products.

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Vitamin B12: Strategies for enhanced production, fortified functional food products and health benefits

Cited by 10 publications

References 105 publications

Bacillus megaterium: Evaluation of Chemical Nature of Metabolites and Their Antioxidant and Agronomics Properties

Bacillus megaterium: Evaluation of Chemical Nature of Metabolites and Their Antioxidant and Agronomics Properties

Research progress on the detection of water-soluble vitamins in food using liquid chromatography

Physicochemical and Microbiological Changes Associated with Processing in Dry-Cured Tuna

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