2015
DOI: 10.1038/nature14227
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Abstract: Rare tautomeric and anionic nucleobases are believed to play fundamental biological roles but their prevalence and functional importance has remained elusive because they exist transiently, in low-abundance, and involve subtle movements of protons that are difficult to visualize. Using NMR relaxation dispersion, we show that wobble dG•dT and rG•rU mispairs in DNA and RNA duplexes exist in dynamic equilibrium with short-lived, low-populated Watson-Crick like mispairs that are stabilized by rare enolic or anioni… Show more

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Cited by 220 publications
(305 citation statements)
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“…2a). Experiments were carried out at near-neutral pH (6.4–6.9) so as to lower the anionic ES2 below detection 8 (Extended Data Fig. 2b).…”
Section: Tilting the Tautomeric Equilibriummentioning
confidence: 99%
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“…2a). Experiments were carried out at near-neutral pH (6.4–6.9) so as to lower the anionic ES2 below detection 8 (Extended Data Fig. 2b).…”
Section: Tilting the Tautomeric Equilibriummentioning
confidence: 99%
“…2c and Supplementary Tables 2,3) 8 . The tautomeric equilibria (Supplementary Table 2) obtained from this analysis and from re-fitting the RD data using a 3-state model with linear topology 25 (wobble⇌G•T enol /U enol ⇌G enol •T/U) are slightly tilted in favor of dG enol •dT in DNA ( K t = 2.1-4.6) whereas the populations of rG enol •rU and rG•rU enol are more comparable in RNA ( K t = 0.5-1.1).…”
Section: Sequence-dependent Genol•t/u⇌g•tenol/uenolmentioning
confidence: 99%
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