Visualizing Intermolecular Interactions in T Cells

Gascoigne, Nicholas R. J.; Ampudia, Jeanette; Clamme, Jean Pierre; Fu, Guo; Lotz, Carina; Mallaun, Michel; Niederberger, Nathalie; Palmer, Ed; Rybakin, Vasily; Yachi, Pia P.; Żal, Tomasz

doi:10.1007/978-3-540-93864-4_2

Cited by 7 publications

(10 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Instead, Forster resonance energy transfer (FRET) can be employed to study colocalization on the scale of a few nanometers and therefore provides information about molecular interactions (Gascoigne et al 2009). Using this technique, FRET was detected between PLC-g1 and either LAT, c-Cbl, Vav or SLP-76, as well as and Nck within microclusters, suggesting direct interactions of these proteins within microclusters Braiman et al 2006).…”

Section: Lat Microclusters: Sites Of Nucleation Of Signaling Complexesmentioning

confidence: 99%

The LAT Story: A Tale of Cooperativity, Coordination, and Choreography

Balagopalan¹,

Coussens²,

Sherman³

et al. 2010

Cold Spring Harbor Perspectives in Biology

152

184

View full text Add to dashboard Cite

The adapter molecule LAT is a nucleating site for multiprotein signaling complexes that are vital for the function and differentiation of T cells. Extensive investigation of LAT in multiple experimental systems has led to an integrated understanding of the formation, composition, regulation, dynamic movement, and function of LAT-nucleated signaling complexes. This review discusses interactions of signaling molecules that bind directly or indirectly to LAT and the role of cooperativity in stabilizing LAT-nucleated signaling complexes. In addition, it focuses on how imaging studies visualize signaling assemblies as signaling clusters and demonstrate their dynamic nature and cellular fate. Finally, this review explores the function of LAT based on the interpretation of mouse models using various LAT mutants.

show abstract

Section: Lat Microclusters: Sites Of Nucleation Of Signaling Complexesmentioning

confidence: 99%

The LAT Story: A Tale of Cooperativity, Coordination, and Choreography

Balagopalan¹,

Coussens²,

Sherman³

et al. 2010

Cold Spring Harbor Perspectives in Biology

152

184

View full text Add to dashboard Cite

show abstract

“…The FRET data, in combination with striking nuclear magnetic resonance measurements, led to the conclusion that CD3ε binds to the inner leaflet of the plasma membrane, burying tyrosine residues that are crucial for activation in the lipid bilayer. FRET has also been used to look at interactions between the TCR and co-receptors 94 .…”

Section: Molecular Interactions and Dynamicsmentioning

confidence: 99%

Imaging techniques for assaying lymphocyte activation in action

Balagopalan¹,

Sherman²,

Barr³

et al. 2010

Nat Rev Immunol

View full text Add to dashboard Cite

Imaging techniques have greatly improved our understanding of lymphocyte activation. Technical advances in spatial and temporal resolution and new labelling tools have enabled researchers to directly observe the activation process. Consequently, research using imaging approaches to study lymphocyte activation has expanded, providing an unprecedented level of cellular and molecular detail in the field. As a result, certain models of lymphocyte activation have been verified, others have been revised and yet others have been replaced with new concepts. In this article, we review the current imaging techniques that are used to assess lymphocyte activation in different contexts, from whole animals to single molecules, and discuss the advantages and potential limitations of these methods.

show abstract

“…These two methods are widely used in the study of protein-protein interactions in vivo, and can directly visualize protein interactions in living cells (58)(59)(60)(61). Based on these assays, we have found that Itk forms head-to-tail dimers only in the plasma membrane, but not in the cytoplasm, suggesting that Itk does not form dimers in the inactive state (57).…”

Section: Conformation Of Activated Itkmentioning

confidence: 90%

Structure and function of Tec family kinase Itk

Kannan

August

2011

BioMolecular Concepts

View full text Add to dashboard Cite

Itk is a member of the Tec family of kinases that is expressed predominantly in T cells. Itk regulates the T cell receptor signaling pathway to modulate T cell development and T helper cell differentiation, particularly Th2 differentiation. Itk is also important for the development and function of iNKT cells. In this review we discuss current progress on our understanding of the structure, activation and signaling pathway of Itk, in addition to inhibitors that have been developed, which target this kinase. We also place in context the function of Itk, available inhibitors and potential use in treating disease.

show abstract

Visualizing Intermolecular Interactions in T Cells

Cited by 7 publications

References 62 publications

The LAT Story: A Tale of Cooperativity, Coordination, and Choreography

The LAT Story: A Tale of Cooperativity, Coordination, and Choreography

Imaging techniques for assaying lymphocyte activation in action

Structure and function of Tec family kinase Itk

Contact Info

Product

Resources

About