Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model

Abstract

The aim of this study was to visualize the tumor propagation and surrounding mucosal field in radiography-based 3D model for advanced stage HNSCC and combine it with HPV genotyping and miRNA expression characterization of the visualized area. 25 patients with T1-3 clinical stage HNSCC were enrolled in mapping biopsy sampling. Biopsy samples were evaluated for HPV positivity and miR-21-5p, miR-143, miR-155, miR-221-5p expression in Digital Droplet PCR system. Significant miRNA expression differences of HPV positive tumor tissue biopsies were found for miR-21-5p, miR-143 and miR-221-5p compared to the HPV negative tumor biopsy series. Peritumoral mucosa showed patchy pattern alterations of miR-21-5p and miR-155 in HPV positive cases, while gradual change of miR-21-5p and miR-221-5p was seen in HPV negative tumors. In our study we found differences of the miRNA expression patterns among the HPV positive and negative tumorous tissues as well as the surrounding mucosal fields. The CT based 3D models of the cancer field and surrounding mucosal surface can be utilized to improve proper preoperative planning. Complex evaluation of HNSCC tissue organization field can elucidate the clinical and molecular differentiation of HPV positive and negative cases, and enhance effective organ saving therapeutic strategies.

Global microRNA expression profile in laryngeal carcinoma unveils new prognostic biomarkers and novel insights into field cancerization

“…Due to this specificity, these patches of field cancerization in laryngeal carcinoma are often resected (sometimes unconsciously), and the stochastic chance of local recurrence due to field cancerization is significantly lower. These wide patches or gradients of dysregulated mucosa far beyond the tumor borders have been well described and visualized by Orosz in another study of oropharyngeal carcinoma 14 . Survival analysis of our data undoubtedly points out miR-93-5p, miR-144-3p, and miR-210-3p as strong predictors for recurrence in laryngeal carcinoma.…”

Expression and molecular regulation of non-coding RNAs in HPV-positive head and neck squamous cell carcinoma

“…Moreover, miRNAs may perform a vital role in oncogenesis by acting as oncomiRNAs or tumor suppressor factors ( 18 , 38 , 43 , 45 , 52 ). Up to the present, miR-22, miR-27, miR-92a, miR-195, and miR-211 have been identified as oncogenic promotion miRNAs in HPV-positive HNSCC ( 18 , 52 , 56 , 57 ), being involved in the occurrence of HNSCC through regulation of their target genes ( 22 , 46 , 54 , 56 , 58 ). For example, miR-21 and miR-155 can promote the proliferation and invasion of OPSCC cells via suppressing downstream target genes, such as phosphatase tensin homolog (PTEN) and signal transducer and activator of transcription (STAT) ( 54 , 59 , 60 ).…”