Visualization of Metastatic Lung Cancer with TiNIR

Abstract

The development of efficient biomarkers and probes for monitoring and treating cancer, specifically metastatic cancer, is a critical research area that can have a significant impact on both patient outcomes and drug discovery. In this context, TiNIR has been developed to detect tumor-initiating cells (TICs), with heme oxygenase 2 (HO2) as a promising therapeutic biomarker for tumor-initiating cells. In this study, TiNIR has demonstrated its effectiveness as an in vivo metastatic lung cancer tracker, highlighting its potential as a valuable tool in cancer research and therapy. The development of innovative approaches that selectively target metastatic cancers represents a promising avenue for improving survival rates and enhancing the quality of life of cancer patients.

Targeting Heme Oxygenase 2 (HO2) with TiNIR, a Theragnostic Approach for Managing Metastatic Non-Small Cell Lung Cancer

“…Cells were stained with TiNIR (1 μM) for 30 min after being washed with PBS and stained with Hoechst 33342 (Thermo, MO, USA) for 10 min. The images were captured with an EVOS M7000 (Thermo, MO, USA) and acquired with a ×40 air objective [ 10 , 18 ].…”

“…The image was analyzed using ImageJ and Celleste image analysis software ver. 5.0 (Thermo, MO, USA) [ 18 ].…”

“…In this study, we investigated the potential utility of HO2 as a therapeutic biomarker for tracking and treating metastatic cancer [ 18 ]. Employing robust lung metastatic cancer models, we evaluated the therapeutic impact of targeting HO2 in cancer metastasis through in vitro and in vivo models.…”