Visual Detection of LPS at the Femtomolar Level Based on Click Chemistry-Induced Gold Nanoparticles Electrokinetic Accumulation
Hanren Chen,
Shiquan Zheng,
Yitong Zhang
et al.
Abstract:Lipopolysaccharide (LPS) presents a significant threat to human health. Herein, a novel method for detecting LPS was developed by coupling hybridization chain reaction (HCR), gold nanoparticles (AuNPs) agglutination (AA) triggered by a Cu(I)-catalyzed azide−alkyne cycloaddition click chemistry (CuAAC), and electrokinetic accumulation (EA) in a microfluidic chip, termed the HCR−AA−EA method. Thereinto, the LPSbinding aptamer (LBA) was coupled with the AuNP-coated Fe 3 O 4 nanoparticle, which was connected with … Show more
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