Virus-specific cytotoxic T-lymphocytes in HIV-2-infected cynomolgus macaques

Voss, Gérald; Nick, Sigrid; Otteken, Ahlert; Lüke, Wolfgang; Stahl–Hennig∥, Christiane; Hunsmann, Gerhard

doi:10.1097/00002030-199210000-00003

Cited by 10 publications

(5 citation statements)

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“…Furthermore, suitable target cells for CTL assay can be readily prepared by pulsing SEA-stimulated, MHC-haploidentical PBMC with heat-inactivated whole SIV,,,. Cytotoxic activities have been widely reported in HIV-infected patients [3,9,24,25,29,39,41,42,46,55,581 and have also been documented during SIV infec- tion [14,15,22,561. Here we used a heat-inactivated virus to generate target cells.…”

Section: Discussionmentioning

confidence: 99%

Detection of simian immunodeficiency virus (SIV)‐specific T‐cell‐mediated cytotoxicity in the peripheral blood from infected cynomolgus monkeys

Mavoungou

Touré

Yaba

et al. 1999

J of Medical Primatology

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We have previously demonstrated that peptide immunization restimulates the memory CD4 T-cell response, but fails to induce cytotoxic T lymphocyte (CTL) in cynomolgus macaques. To examine the nature of protective immunity to simian immunodeficiency virus (SIV) in this study, freshly isolated peripheral blood mononuclear cells (PBMC) from four infected juvenile cynomolgus macaques and from three uninfected control macaques were assessed for CTL activity monthly for 9 consecutive months, beginning 1 month after detection of infection. Target cells consisted of major histocompatibility (MHC) haploidentical parental PBMC which were stimulated with mitogen and then pulsed with heat-killed SIVcyn. CTL activity was demonstrated in PBMCs from all four infected animals. The effector cells are T cells which mediate cytotoxicity against SIVcyn-pulsed target cells in an MHC-restricted manner. Furthermore, the cytotoxicity is virus specific and predominantly, if not exclusively, mediated by CD8+ T cells; it is also MHC class I restricted. Incubation of target cells with pepstatin A during antigen pulsing prior to the cytotoxic assay inhibited target cell generation, suggesting that viral antigens are processed via an endocytic pathway.

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Section: Discussionmentioning

confidence: 99%

Detection of simian immunodeficiency virus (SIV)‐specific T‐cell‐mediated cytotoxicity in the peripheral blood from infected cynomolgus monkeys

Mavoungou

Touré

Yaba

et al. 1999

J of Medical Primatology

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“…PBMC from Mm L3 and L28 were stimulated with p9CD and p6B, respectively. Prior to assessment in a 51 Cr release assay the effector cells were separated with CD8-specific immunomagnetic beads (Dynal, Lake Success, N.Y.) (30). The resulting cell populations were examined for lysis of autologous target B-LCL pulsed with p9B (dotted), p9CD (black), p6A (white), or p6B (grey) at an E/T ratio of 2.5:1.…”

Section: Figmentioning

confidence: 99%

Definition of human immunodeficiency virus type 1 gp120 and gp41 cytotoxic T-lymphocyte epitopes and their restricting major histocompatibility complex class I alleles in simian-human immunodeficiency virus-infected rhesus monkeys

Voss

Letvin

1996

J Virol

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With the development of chimeric simian-human immunodeficiency virus (SHIV)-infected macaques as a model for assessing novel human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env)-based vaccine strategies for preventing HIV-1 infection in man, it will be important to determine HIV-1 Env-specific cytotoxic T-lymphocyte (CTL) responses in vaccinated and virus-infected monkeys. To facilitate performing such CTL studies, we have defined two HIV-1 Env CTL epitopes in SHIV-infected rhesus monkeys and characterized the major histocompatibility complex (MHC) class I alleles that bind these Env peptide fragments and present them to CTL. A 9-amino-acid (aa) fragment of HIV-1 gp41 (p6B, aa 553 to 561) is presented to CD8 ؉ CTLs of SHIV-infected animals by the rhesus monkey HLA-B homolog molecule Mamu-B*12. An 8-aa HIV-1 gp120 peptide (p9CD, aa 117 to 124) represents a CTL epitope in rhesus monkeys restricted by the HLA-A homolog MHC allele Mamu-A*08. This gp120 CTL epitope is fully conserved in all simian immunodeficiency virus, HIV-1, and HIV-2 isolates that have been sequenced to date and exhibits functional crossreactivity. Screening of 14 unselected rhesus monkeys for expression of the two novel MHC class I alleles revealed the presence of each of the alleles in more than 40% of the animals. The characterization of the two HIV-1 Env CTL epitopes and their restricting MHC class I alleles will provide a basis for studying vaccine-and virus-elicited cytotoxic effector cell responses in rhesus monkeys.

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“…The absorbance values measured were used to calculate the specific lysis. Lysis found to be 10% above the non-specific lysis of wtVV-infected target cells was regarded as SIV-specific (Voss et al, 1992c). Determination of urinary neopterin, a non-specific marker for immune activation (Fuchs et aL, 1988) was carried out essentially as described by Fendrich et al (1989).…”

Section: Cellular Immune Responsementioning

confidence: 99%

Repeated exposure of rhesus macaques to low doses of simian immunodeficiency virus (SIV) did not protect them against the consequences of a high-dose SIV challenge

Dittmer

Stahl–Hennig∥

Coulibaly

et al. 1995

Journal of General Virology

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Virus-specific cytotoxic T-lymphocytes in HIV-2-infected cynomolgus macaques

Cited by 10 publications

References 0 publications

Detection of simian immunodeficiency virus (SIV)‐specific T‐cell‐mediated cytotoxicity in the peripheral blood from infected cynomolgus monkeys

Detection of simian immunodeficiency virus (SIV)‐specific T‐cell‐mediated cytotoxicity in the peripheral blood from infected cynomolgus monkeys

Definition of human immunodeficiency virus type 1 gp120 and gp41 cytotoxic T-lymphocyte epitopes and their restricting major histocompatibility complex class I alleles in simian-human immunodeficiency virus-infected rhesus monkeys

Repeated exposure of rhesus macaques to low doses of simian immunodeficiency virus (SIV) did not protect them against the consequences of a high-dose SIV challenge

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