Virus-induced Gene Silencing in Streptocarpus rexii (Gesneriaceae)

Nishii, Kanae; Fei, Yue; Hudson, Andrew; Möller, Michael; Molnár, Attila

doi:10.1007/s12033-020-00248-w

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…Most of this work has been carried out on the ornamental Streptocarpus ionanthus (formerly Saintpaulia ionantha , African Violet) for breeding purposes: here, agrobacterium‐mediated transformation was successful (Kushikawa et al, 2001 ), as well as chemical mutagenesis with ethylmethanesulfonate, colchicine, and physical mutagenesis through exposure to different types of irradiation (da Silva et al, 2017 ). In S. rexii , a successful virus‐induced gene‐silencing protocol was recently reported (Nishii et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The first genome for the Cape Primrose Streptocarpus rexii (Gesneriaceae), a model plant for studying meristem‐driven shoot diversity

et al. 2022

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Cape Primroses ( Streptocarpus , Gesneriaceae) are an ideal study system for investigating the genetics underlying species diversity in angiosperms. Streptocarpus rexii has served as a model species for plant developmental research for over five decades due to its unusual extended meristem activity present in the leaves. In this study, we sequenced and assembled the complete nuclear, chloroplast, and mitochondrial genomes of S. rexii using Oxford Nanopore Technologies long read sequencing. Two flow cells of PromethION sequencing resulted in 32 billion reads and were sufficient to generate a draft assembly including the chloroplast, mitochondrial and nuclear genomes, spanning 776 Mbp. The final nuclear genome assembly contained 5,855 contigs, spanning 766 Mbp of the 929‐Mbp haploid genome with an N50 of 3.7 Mbp and an L50 of 57 contigs. Over 70% of the draft genome was identified as repeats. A genome repeat library of Gesneriaceae was generated and used for genome annotation, with a total of 45,045 genes annotated in the S. rexii genome. Ks plots of the paranomes suggested a recent whole genome duplication event, shared between S. rexii and Primulina huaijiensis . A new chloroplast and mitochondrial genome assembly method, based on contig coverage and identification, was developed, and successfully used to assemble both organellar genomes of S. rexii . This method was developed into a pipeline and proved widely applicable. The nuclear genome of S. rexii and other datasets generated and reported here will be invaluable resources for further research to aid in the identification of genes involved in morphological variation underpinning plant diversification.

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Section: Introductionmentioning

confidence: 99%

The first genome for the Cape Primrose Streptocarpus rexii (Gesneriaceae), a model plant for studying meristem‐driven shoot diversity

et al. 2022

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“…Another TRV-VIGS study in Streptocarpus rexii (Gesneriaceae) showed that the efficacy of TRV-VIGS showed a correlation with the age of S. rexii seedlings (Burch-Smith et al, 2006). The younger the plants were when infected with TRV, the more efficient the silencing of the SrPDS gene (Nishii et al, 2020).…”

Section: Age or Development Stages Of Infected Plantsmentioning

confidence: 99%

“…A study demonstrated that the TRV-based VIGS technique could be adapted for high-throughput functional characterization of genes in the perennial tree peony (Xie et al, 2019). Nishii et al (2020) utilized the broad host range TRV vector to target the SrPDS gene of S. rexii (Gesneriaceae) by agroinfiltration and sap inoculation and successfully obtained SrPDS silenced plants.…”

Section: Ornamental Plantsmentioning

confidence: 99%

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

et al. 2021

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As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.

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“…In Catharanthus roseus [33], the TRV-CrPDS system was used to infect roots, stems, leaves, and flowers, and it was found that all tissues showed obvious albino, and the phenotypes of leaves and flowers were more obvious. Nishii et al used a TRV vector with a wide host range to silence the SrPDS gene in Streptocarpus rexii through Agrobacterium inoculation, and finally obtained silenced plants with albino phenotypes [34]. These examples demonstrate the wide application of the TRV-PDS system.…”

Section: Introductionmentioning

confidence: 99%

Establishing a Virus-Induced Gene Silencing System in Lycoris chinensis

Cheng

Shu

Wang

et al. 2023

Plants

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Lycoris is an important plant with both medicinal and ornamental values. However, it does not have an efficient genetic transformation system, which makes it difficult to study gene function of the genus. Virus-induced gene silencing (VIGS) is an effective technique for studying gene functions in plants. In this study, we develop an efficient virus-induced gene-silencing (VIGS) system using the leaf tip needle injection method. The widely used TRV vector is constructed, and the Cloroplastos Alterados 1 (CLA1) and Phytoene Desaturase (PDS) genes are selected as visual indicators in the VIGS system. As a result, it is observed that leaves infected with TRV-LcCLA1 and TRV-LcPDS both show a yellowing phenotype (loss of green), and the chlorosis range of TRV-LcCLA1 was larger and deeper than that of TRV-LcPDS. qRT-PCR results show that the expression levels of LcCLA1 and LcPDS are significantly reduced, and the silencing efficiency of LcCLA1 is higher than that of LcPDS. These results indicate that the VIGS system of L. chinensis was preliminarily established, and LcCLA1 is more suitable as a gene-silencing indicator. For the monocotyledonous plant leaves with a waxy surface, the leaf tip injection method greatly improves the infiltration efficiency. The newly established VIGS system will contribute to gene functional research in Lycoris species.

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Virus-induced Gene Silencing in Streptocarpus rexii (Gesneriaceae)

Cited by 6 publications

References 21 publications

The first genome for the Cape Primrose Streptocarpus rexii (Gesneriaceae), a model plant for studying meristem‐driven shoot diversity

The first genome for the Cape Primrose Streptocarpus rexii (Gesneriaceae), a model plant for studying meristem‐driven shoot diversity

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

Establishing a Virus-Induced Gene Silencing System in Lycoris chinensis

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